GABA and glycine Flashcards
explain the 2 main methods with which inhibitory mechanisms work
with ligand-binding receptors, 2 things can happen once the ion channels open
- influx of negatively charged ions
- open potassium channels, potassium efflux
name 2 inhibitory neurotransmitters
GABA and glycine
inhibitory neurotransmitters eg. GABA cause what?
neuronal membrane hyperpolarisation
what do hyper polarisations result in?
an influx of negative charge or an efflux of positive charge
GABA is the major…..
…..inhibitory neurotransmitter in the central nervous system
GABA synthesis
synthesised in nerve terminals from glutamate
- glutamate decarboxylase converts glutamate to GABA, vitamin B6 acts as a cofactor
- lose a carboxylic acid when going from glutamate to GABA
why is it clever that GABA is synthesised from glutamate?
means that if a cell can make the excitatory neurotransmitter, it will also always be able to make the inhibitory neurotransmitter, ensuring the organism can perform both excitatory and inhibitory effects.
GABA storage
transported into vesicles by VIAAT (VGLUT is glutamate equivalent)
shape of GABA vesicles
oval
shape of glutamate vesicles
round
how does GABA mediate its response?
via GABAa and GABAb receptors
GABAa receptor structure and GABA binding
- ligand gated chloride channel
- pentameric structure (key difference to the glutamatergic ones, which were all tetramers)
- variety of subunits (6 alpha, 3 beta, 3 gamma, also pi, delta, epsilon)
- most common configuration is 2 alpha, 2 beta and 1 gamma
- GABA binds extracellularly at the interface between the alpha and beta receptors (2 interfaces)
- need 2 molecules of GABA binding to the alpha beta interface in order for channels to open
where are GABAa receptors found?
-majority of GABAa receptors are found post-synaptically, but they can be found pre-synaptically and also on various glial subtypes
why is the GABAa receptor the target of many different types of drugs?
because it has multiple binding sites
-eg. Benzodiazepine binding sites, alpha gamma interface
GABAb receptor structure
- GPCR
- extracellular N-terminal “venus flytrap” domain
- 7 TM region made of alpha helices
- internal c-terminal domain that interacts with the g-proteins
- GABA receptor forms dimers, can be heteromers of 2 different subtypes of GABAb