Furci Exam 1 Study Guide Flashcards
Glycine
Two H atoms connected to alpha carbon
Aromatic R Groups
Arrows and TTPs (Indians)
Tyrosine
Tryptophan
Phenylalaine
Positively Charged R Groups (basic)
(LArHis, a Positive guy)
Lysine
Arginine
Histidine
Negatively Charged R Groups (acidic)
(Negative NAG)
Aspartate
Glutamate
Polar, Uncharged R Groups
(STAG)
Serine
Threonine
Asparagine
Glutamine
NonPolar Aliphatic
{Gava-lip}
Glycine Alanine Valine Leucine Isoleucine Proline
Sequence of a chain of amino acids
Primary Structure
Alpha helix and beta sheaths with H-bond interactions and polypeptide backbone
Secondary Structure
Alpha helix and beta-sheaths all connected by disulfide bonds, H bonds, salt bridges. Hydrophobic structure
Tertiary Structure
Assembly of many polypeptide chains into an intact tetrameric protein
Quaternary Structure
State in which a particular molecule holds an ionic charge
Ionization State
Mathematical explanation of dissociation of a weak acid
Henderson-Hasselbach Eq.
Molecule that exists with both positive and negative charge
Zwitterion
pH where molecule has no net charge
Isoelectric Point (pI)
Produced when 2 amino acids are bonded together
Peptide
Autoimmune disorder where immune cells attach and destroy exocrine glands that make tears/saliva
Sjogren’s Syndrome
Disrupts fibrillin (fibers of lens) which is rich in cysteine residues
Homocysteinuria
Lens dislocation at birth
Sulfite Oxidase Deficiency
Mutations in fibrillin gene (lens dislocation)
Marfan’s Syndrome
Human prion disease. Abnormal PrPC accumulation. Alpha helices switched to B pleated sheets.
Creutzfeldt-Jakob Disease (Mad Cow Disease)
Body not breaking down bilirubin and excreting; green teeth
Hyperbilirubinemia
Natural structure derived from castor made up of two chains (A penetrate/kill, B binds)
Ricin
Chromatography (protein purify) methods
Size Exclusion Chrom (SEC)- AKA gel filtration; small molecules slow
Ion Exchange Chrom (IEC)- uses resins, pH
Affinity Chromatography (biorecognition/ligand specificity)- best selectivity, what sticks is of interest
Inhibitors of oxidative Metabolism
Oligomycin
Disrupt the ETC by transporting H back across membrane, stimulation respiration (weight loss supplements)
Uncouplers
Which enzymes are e- transport carriers?
Complexes 1 and 3 transport e- to 4, which uses them to reduce oxygen
Which enzymes are involved in transporting protons?
Complexes 1, 3, and 4 make proton gradient that 5 uses to make ATP
Sulfur-containing R groups (AA)
Cysteine
Methionine
