Furci Exam 1 Study Guide

Question 1

Glycine

Answer 1

Two H atoms connected to alpha carbon

Question 2

Aromatic R Groups

Answer 2

Arrows and TTPs (Indians)

Tyrosine
Tryptophan
Phenylalaine

Question 3

Positively Charged R Groups (basic)

Answer 3

(LArHis, a Positive guy)

Lysine
Arginine
Histidine

Question 4

Negatively Charged R Groups (acidic)

Answer 4

(Negative NAG)

Aspartate
Glutamate

Question 5

Polar, Uncharged R Groups

Answer 5

(STAG)

Serine
Threonine
Asparagine
Glutamine

Question 6

NonPolar Aliphatic

Answer 6

{Gava-lip}

Glycine
Alanine
Valine
Leucine
Isoleucine
Proline

Question 7

Sequence of a chain of amino acids

Answer 7

Primary Structure

Question 8

Alpha helix and beta sheaths with H-bond interactions and polypeptide backbone

Answer 8

Secondary Structure

Question 9

Alpha helix and beta-sheaths all connected by disulfide bonds, H bonds, salt bridges. Hydrophobic structure

Answer 9

Tertiary Structure

Question 10

Assembly of many polypeptide chains into an intact tetrameric protein

Answer 10

Quaternary Structure

Question 11

State in which a particular molecule holds an ionic charge

Answer 11

Ionization State

Question 12

Mathematical explanation of dissociation of a weak acid

Answer 12

Henderson-Hasselbach Eq.

Question 13

Molecule that exists with both positive and negative charge

Answer 13

Zwitterion

Question 14

pH where molecule has no net charge

Answer 14

Isoelectric Point (pI)

Question 15

Produced when 2 amino acids are bonded together

Answer 15

Peptide

Question 16

Autoimmune disorder where immune cells attach and destroy exocrine glands that make tears/saliva

Answer 16

Sjogren’s Syndrome

Question 17

Disrupts fibrillin (fibers of lens) which is rich in cysteine residues

Answer 17

Homocysteinuria

Question 18

Lens dislocation at birth

Answer 18

Sulfite Oxidase Deficiency

Question 19

Mutations in fibrillin gene (lens dislocation)

Answer 19

Marfan’s Syndrome

Question 20

Human prion disease. Abnormal PrPC accumulation. Alpha helices switched to B pleated sheets.

Answer 20

Creutzfeldt-Jakob Disease (Mad Cow Disease)

Question 21

Body not breaking down bilirubin and excreting; green teeth

Answer 21

Hyperbilirubinemia

Question 22

Natural structure derived from castor made up of two chains (A penetrate/kill, B binds)

Answer 22

Ricin

Question 23

Chromatography (protein purify) methods

Answer 23

Size Exclusion Chrom (SEC)- AKA gel filtration; small molecules slow

Ion Exchange Chrom (IEC)- uses resins, pH

Affinity Chromatography (biorecognition/ligand specificity)- best selectivity, what sticks is of interest

Question 24

Inhibitors of oxidative Metabolism

Answer 24

Oligomycin

Question 25

Disrupt the ETC by transporting H back across membrane, stimulation respiration (weight loss supplements)

Answer 25

Uncouplers

Question 26

Which enzymes are e- transport carriers?

Answer 26

Complexes 1 and 3 transport e- to 4, which uses them to reduce oxygen

Question 27

Which enzymes are involved in transporting protons?

Answer 27

Complexes 1, 3, and 4 make proton gradient that 5 uses to make ATP

Question 28

Sulfur-containing R groups (AA)

Answer 28

Cysteine

Methionine