Forensic Biology Flashcards
1
Q
Three substances that need to be added during PCR
A
Primers
Nucleotides
DNA polymerase
2
Q
What temperatures are required in a PCR?
A
95°C –> 55°C –> 70°C
3
Q
How many times must the PCR be done?
A
Many times
4
Q
Why isn’t DNA polymerase provided from human sources in PCR?
A
Enzymes would denature above 37°C due to changing shape of the active site
PCR done at a minimum temperature of 55°C
5
Q
Why would the species of a plant not be able to be identified from xylem material using PCR?
A
Xylem made up of dead material so no DNA present
