Food Chemistry Flashcards
What compund in chocolate stimulates the central nervous system and also makes dogs sick?
theobromine
What chemical hormone do fruits release upon ripening?
ethylene
Chlorophyll molecule reacting with hydrogen exhibits what shade of green?
olive green
What two common compounds are used together as an antioxidative system?
Ascorbic Acid and Citric Acid
What is the name of the final degradation compound of chlorophyll?
Pheophorbide
What is an isomer that is cheaper than Vitamin C?
Erythorbic Acid
What is the enzyme responsible for browning in apples and lettuce?
Polyphenol Oxidase
What are the acids present in fish oil?
Arachidonic Acid, DHA, and EPA
What is surimi?
A concentrate of microfibrillar proteins of fish muscle.
What is Carboxyl-methyl cellulose often used for?
Thickener in foods (and stabilizer)
Does Kosmotrope ion discourage water structure?
No (they cause water molecules to favorably interact)
What is the main class of color compounds found in raspberries?
Anthocyanins
What is the major color compound in tomatoes?
Lycopene
What is the structural difference between chlorophyll and chlorophyllide?
Chlocophyllide does not have a phytol tail
What happens to chlorophyll on heating?
Pheophytin forms
Alar is the trade name for what chemical compound?
Daminozide
How many polypeptide chains make up an antibody?
Four
What is the winterization process for an oil?
Cooling the oil and filtering of the solids formed so that long chained saturated fats do not crystallize at low temperatures.
What is the structural difference between chlorophyll and pheophytin?
Pheophytin does not have magnesium in porphyrin ring.
Peptide bond formation results in the formation of what secondary compound?
water
What metabolic end product does the Voges-Proskauer test detect?
Acetoin in a baceterial broth culture
Will lipid oxidation be higher at a water activity of .05 or .5?
0.05
How many carbon atoms and how many double bonds are there in arachidonic acid?
20 carbons and 4 double bonds
What are the three stages of oxidative rancidity?
Initiation, Propagation, and Termination
What ketohexose is most significant to food chemists?
Fructose
How many carbon atoms are there in the fatty acid myristic acid?
14
What is the Peroxide Value a measure of?
Fat Oxidation
Waxes are composed of what two basic chemical entities?
Fatty Acid (Ester linked) to Fatty Alcohol
Name three effects of protein denaturation.
Decreased solubility, altered water-binding capacity, loss of biological activity, increased susceptibility to attack by proteases, increased intrinsic viscosity, inability to crystallize.
Name 3 physical agents capable of denaturing proteins.
Temperature Changes, Mechanical Actions, Change in pH, Irradiation, Sound Waves, and Exposure to Mineral Salt and Metals
Name two main gluten proteins.
Gliadins and Glutenins
How do sodium chloride and sucrose affect foam stability?
Sodium chloride reduces foam stability and sucrose improves foam stability.
Name three factors useful in controlling enzyme activity.
Temperature, Water Content and Activity, Extremes of pH, Chemicals, Alterations of Substrates, Alterations of Products, Preprocessing Controls
Name 3 chemical agents capable of denaturing proteins.
Acids, Alkalis, Metals, Organic Solvents, and Aqueous Solutions of Organic Solvents
Name 5 proteins with good foaming properties.
Egg white proteins, the globin part of hemoglobin, bovine serum albumin, gelatin, whey proteins, casein micelles, B-casein, wheat proteins, soy proteins, and some protein hydrolyzates.
Name 3 reasons it is advantageous to obtain enzymes from microorganisms.
Microorganisms are very versatile, MO’s can be altered by mutations or genetic engineering to produce a greater quantity of enzyme or different enzymes, recovery of enzymes is often easy, readily available, high rate of growth and enzyme production.
What effect would the addition of sodium bicarbonate have to chocolate?
Smoother, less acid and less bitter chocolate flavor, darker color, and improved solubility (elevated pH enhances sugar-amino browning reactions and polymerization of flavonoids).
Name 5 chelating agents used in the food industry.
EDTA, citric acid, malic acid, tartaric acid, oxalic acid, succinic acid, adenosine triphosphate, pyrophosphate, porphyrins, and proteins.
Name 2 heat stable enzymes in food that are important to the quality of aseptic products.
Lipase, lipoxygenase, catalase, chlorophylase, peroxidase, pectinase, protease, ascorbic acid oxidase, alkaline phosphotase.
A chlorophyll molecule reacting with magnesium exhibits what shade of green?
Bright green
Name one way foam stability can be assessed.
The degree of liquid drainage or foam collapse (foam reduction) after a given time, the time for total or half-drainage (or half-reduction in volume), or the time before drainage starts.
Name 4 advantages of using enzymes in food processing.
They are natural, nontoxic substances, generally catalyze without unwanted side-effects, active under very mild conditions of temperature and pH, active at low concentrations, rate of reaction can be controlled, can be inactivated after reaction.
S-(1-propenyl)-L-cysteine sulfoxide is the chemical precursor to the major flavor compounds in what foodstuff?
Onions ( or leeks)
What kinds of food emulsions are?
(1) Butter
(2) Mayonnaise
(3) Milk
Butter= water in oil Mayonnaise= oil in water Milk= oil in water
What is the fundamental driving of electrophoresis?
The speed of a molecule is proportional to the surrounding voltage gradient.
How can a molecular weight of protein, DNA, and RNA be determined?
For proteins: SDA-PAGE.
For DNA & RNA: Agarose gel.
How does HPLC differ from FPLC?
HPLC has higher pressure than FPLC
What are two common reducing agents that break disulfide bonds in proteins?
2-mercaptoethanol (2-ME), dithiothreitol (DDT)
In polyacrylamide gel electrophoresis (PAGE) what purpose do ammonium persulfates (APS) and TEMED serve?
They act as catalysts, speeding the polymerization of acrylamide.
What does the acronym NPN stand for?
Non-protein nitrogen
Name three nonpolar polar, and charged amino acids.
Nonpolar: (Valine, Alanine, Leucine, Isoleucine, Proline, Tryptophan) Polar: (Glycine, Serine, Cystein, Threonine, Tyrosine) Charged: (Lysine, Arginine, Histidine, Aspartic Acid, Glutamic Acid)
What name is given to the small circular DNA molecules that carry genetic information independently of the main bacterial chromosome?
plasmids
In genetics what is conjugation?
DNA transferred by cell to cell contact (bacterial sex).
Which will be coldest at its freezing point: 1M dispersion of amylose 1M solution of NaCl, distilled water, or 1M solution of sucrose?
NaCl (it dissociated to a 2M solution)
What metabolic disorder prevents conversion of phenulalanine to tyrosine by normal metabolic pathways?
Phenylketonuria
What does MSG stand for?
Monosodium Glutamate
What happens to a bottle of wine if before you bottle it you forget to put the stuff in it that prevents it from fermenting?
It explodes
If you take 20 ml of .1 M NaOH to titrate 40 mL of an HCL solution to the penolphthalein end point what is the molarity of the HCL solution?
.05 M HCL
What does the “c” stand for in the equation E=mc2?
Speed of Light
20 grams of amylose is added to 80 grams of water in a beaker; the boiling point is measured with an ordinary mercury-in-glass thermometer. What is the observed boiling point of the material in the beaker?
100 Degrees Celsius (it is not a true solution-there is no boiling point of elevation)
If oxygen attacks an unsaturated fatty acids at the double bond what is the resultant product?
Two aldehydes
In the enzyme Taq Polymerase what does Taq stand for?
Thermus Aqauticus
What are the primary secondary, tertiary, and quaternary structures?
Primary-amino acid sequence. Secondary-alpha-helix, beta sheet. Tertiary-one complete protein chain with cross bonds linking it together. Quaternary-four separate protein chains assembled as one.
Define molarity, molality, and normality.
Molarity is moles of substance per liter solution. Molality is moles of substance per kg solvent. Normality is equivalents of substance per liter solution.
How is pH defined?
Negative log of the hydrogen concentration (mol/l).
What charge will a protein have when the pH of its solution is below, at, and above its pKa?
pH below positive charge; pH at no charge; pH above negative charge
What does the saponification value measure and how is it measured?
Number of fatty acids present; mg KOH needed to saponify 1 g fat.
What does Acid Degree Value measure and how is it measured?
Amount of free fatty acids present; mg KOH needed to neutralize the free fatty acids in 1 g of fat.
Water
A) Is the major component of most foods.
B) Controls shelf-life or storage capability
C) Regulates chemical and microbiological reactions
D) All of the above
D) All of the above
Which of the following foods contain the lowest content of water? A) Fresh Carrots B) Fresh Tomatoes C) Whole Milk D) Fresh Celery
C) Whole Milk
Which of these food additives could be used to prevent cocoa particles from settling in chocolate milk? A) BHA (butylated hydroxyanisole B) Carrageenan C) Thiamine Hydrochloride D) Citric Acid
B) Carrageenan
Which of the following examples of browning is enzymatic in nature? 1. Browning of sugars as a result of heating 2. Browning of freshly cut surfaces of fruits and vegetables 3. Browning of bread during toasting 4. Browning of potato chips
Browning of freshly cut surfaces of fruits and vegetables.
What would be a safe processing time for heat processing green beans in a boiling water bath? A. 30 minutes B. 300 minutes C. Depends on the size of the food container D. None of the above
D: None of the above
Which of these foods is not appropriate to this list? A) Salami B) Yogurt C) Orange Juice D) Beer
A: Orange Juice
To which of the following four basic tastes are humans most sensitive? A) Bitter B) Sweet C) Sour D) Salty
Bitter
At what pH is benzoic acid most effective?
pH 3-5
What is acid proteolysis?
Degradation of protein and production of acid from sugar at the same time.
What is catalase?
An enzyme that converts hydrogen peroxide to water plus oxygen.
What reaction does the enzyme “reverse transcriptase” catalyze?
RNA transcribed to DNA
What pigment was first designated as anthocyanins?
Blue pigment in flowers
What is the chemical nature of anthocyanins?
Glycosides of anthocyanidins
Name a naturally occurring protein sweetener.
Thaumautin
What is the chemical nomenclature for caffeine?
1, 3, 7-trimethylxanthine
Name three non-nutritive sweeteners approved for use in USA?
Acsulfame-K, Saccharin, Succralose, Aspartame
Which two amino acids is the aspartame derived from?
Aspartic Acid and Phenylalanine
Are sorbitol and mannitol sweet?
yes
What are the fructose contents in commercial HFCS products.
45%, 52%, 90%
What are the limiting amino acids in corn, potato, and green peas?
Corn- Lysine, Potato-Methionine, Green Peas-Methionine
Which amino acid has the greatest molecular weight: tryptophan or tyrosine?
Tryptophan
Give one word for a carbon atom involved in hemiacetal or acetal formation.
Anomeric
What degradative enzyme causes browning in apples, pears, bananas, and peaches?
Polyphenoloxidase
Name the two components that comprise the relaxing factor in muscle fibers.
Troponins and Tropomyosin
An emulsifier with a high hydrophilic-lipophilic balance (HLB) value would promote which type of emulsion: oil in water or water in oil?
Oil in Water
What is the protein efficiency ratio (PER) of casein?
2.5
What two enzymes degrade pectic substances?
Pectin methylesterase & polygalacturonase
Match the number of carbon atoms with the common name of these saturated fatty acids. Carbon #: 4:0 10:0, 12:0,14:0, 16:0, 18:0Common Name: Myristic, Butyric, Capric, Lauric, Stearic, Palmitic
4:0 (Butyric), 10:0 (Capric), 12:0 (Lauric), 14:0 (Myristic),16:0 (Palmitic), 18:0 (Stearic)
What is a polymorphism?
Solid phases of the same chemical composition that differ by crystalline structure, but yield identical liquid phases upon melting.
Name 3 factors influencing the consistency of commercial fats.
Proportion of solids in the fat, number, size, and kinds of crystals, viscosity of the liquid, temperature treatment, and mechanical working.
What does HLB stand for and what does a high and low HLB value indicate?
HLB (hydrophilic-lipophilic balance); a low value (3-6) promotes water in oil emulsions and a high value (8-18) promotes oil in water emulsions.
Name 3 factors influencing the rate of lipid oxidation in foods.
Fatty acid composition, free fatty acids versus the corresponding glycerols, oxygen concentration, temperature, surface area, moisture, pro-oxidants, antioxidants
Name two reasons why fats are hydrogenated.
It allows the conversion of liquid oils into semisolid or plastic fats and it improves the oxidative stability of the fat.
Name three compounds capable of poisoning the catalyst during commercial hydrogenation.
Phospholipids, water, sulfur compounds, soaps, partial glycerol esters, carbon dioxide, and mineral acids.
Name the three positively charged amino acids.
Lysine, Arginine, and Histidine
If the absorption of a sample is being determined spectrophotometrically at 420 nm what color is the sample?
Blue/purple
What enzyme is most responsible for browning bananas?
Polyphenol Oxidase
During freezing what are the two stages in the crystallization process?
Nucleation or crystal formation and crystal growth.
In what food is butyric acid typically found?
milk
What is the name given to a mixture of equal parts of Darel Lisomers?
racemix mixtures
What enzyme causes hyrolytic rancidity in milk?
lipase
Name two enzymes that are involved in softening fruits.
Pectin Esterase, Polygalacturonase, Pectin Layase
Name the strongest biochemical bond and the weakest biochemical bond.
Covalent (strongest) and Hydrophobic (Weakest)
What is the enzyme that softens tomatoes?
Pectin Esterase
Name a commonly used household chemical leavening agent and the gas it generates.
Sodium bicarbonate generates CO2
Name two non-enzymatic browning reactions.
Carmelization and Maillard
What are the three main steps in the autooxidation of lipids?
Initiation, Propagation, Termination
What are the only phenols approved for use in the microbiological preservation of foods?
Parabens
What is the common name for 9 12, cis-cis-octa dienoic acid?
Linoleic Acid
What are 3 mesomorphic structures associated with lipids in the liquid state?
Lamellar; Hexagonal I, II; Cubic
What are proteins which are soluble in 50-80% ethanol known as?
Prolamines
What are colloids that are stabilized by a layer of solvation called?
Emulsoids
What are soluble polymers of anhydro-galacturonic acid and its esters called?
Pectins
What is the geometric configuration of double bonds in most natural fatty acids?
cis
The undesirable clarification of orange juice or tomato juice (precipitation of pulp) is caused by what enzymes?
Pectin methyl esterase or galacturonase
What is the movement of molecules through a semi permeable membrane from a region of low to high solute concentration called?
Osmosis
What is the name of the structural carbohydrate found in lobsters crab, shellfish, and insects?
Chitin
What chemical compound is responsible for the “hot” principle of jalepeno peppers?
Capsaicin
When heated ammonium bicarbonate decomposes to form what end products?
Ammonia, Water, Carbon Dioxide
What two types of compounds are joined with an ester bond to make a wax?
Fatty Acid and Alcohol
What compounds oxidize to form brown color in fruits and vegetables?
Polyphenols
What toxic pigment is found in cottonseed endosperm?
Gossypol (it is removed during the oil refining process)