Flow Questions

Question 1

What are the three applications of cytometry?

Answer 1

Diagnosis and subclassifications of leukemias and lymphomas. (2) Detection on minimal residual disease before relapse (3) additional info in challenging cases

Question 2

Brief flow definition.

Answer 2

Fluorescent Monoclonal antibodies to antigens expressed at specific stages of normal hematopoietic maturation. Single file and shot at with laser and then detected.

Question 3

Define hydrodynamic focusing

Answer 3

makes flow cytometer more accurate for determining size

Question 4

Define gating

Answer 4

selection of a certain population

Question 5

Define data acquisition gating

Answer 5

Comprehensive analysis of all cells, internal positive and negative controls, detection of unexpected abnormal populations

Question 6

Define live gating

Answer 6

Selection of a certain cell population

Question 7

Forward scatter?

Answer 7

The size of a cell

Question 8

Side scatter?

Answer 8

The complexity of the cytoplasm

Question 9

Look at normal flow scatter

Answer 9

Page 17 PPT one

Question 10

Immature cell marker?

Answer 10

34, 117, TdT

Question 11

Granulocytic/monocytic marker?

Answer 11

34, 33, 15, 13, 14

Question 12

Erythroid precursor marker?

Answer 12

71, glycophorin A

Question 13

Megakarocytes marker?

Answer 13

41,42,61

Question 14

B-Cell marker?

Answer 14

19,20 Kappa/Lambda light chains

Question 15

T cell markers?

Answer 15

2,5,7,4,8

Question 16

What does CD stand for?

Answer 16

Cluster designation or cluster of differentiation

Question 17

What marker is on all cells?

Answer 17

CD 45

Question 18

Where are the sites of production for CLL and SLL?

Answer 18

CLL is BM, SLL is lymph nodes

Question 19

How does a germinal center differ from a follicle?

Answer 19

Germinal centers have been presented with an antigen

Question 20

What describes a lymphoma?

Answer 20

Lymphomas involve lymphocytes and typically present in lymph nodes