Flow Questions Flashcards
What are the three applications of cytometry?
Diagnosis and subclassifications of leukemias and lymphomas. (2) Detection on minimal residual disease before relapse (3) additional info in challenging cases
Brief flow definition.
Fluorescent Monoclonal antibodies to antigens expressed at specific stages of normal hematopoietic maturation. Single file and shot at with laser and then detected.
Define hydrodynamic focusing
makes flow cytometer more accurate for determining size
Define gating
selection of a certain population
Define data acquisition gating
Comprehensive analysis of all cells, internal positive and negative controls, detection of unexpected abnormal populations
Define live gating
Selection of a certain cell population
Forward scatter?
The size of a cell
Side scatter?
The complexity of the cytoplasm
Look at normal flow scatter
Page 17 PPT one
Immature cell marker?
34, 117, TdT
Granulocytic/monocytic marker?
34, 33, 15, 13, 14
Erythroid precursor marker?
71, glycophorin A
Megakarocytes marker?
41,42,61
B-Cell marker?
19,20 Kappa/Lambda light chains
T cell markers?
2,5,7,4,8
What does CD stand for?
Cluster designation or cluster of differentiation
What marker is on all cells?
CD 45
Where are the sites of production for CLL and SLL?
CLL is BM, SLL is lymph nodes
How does a germinal center differ from a follicle?
Germinal centers have been presented with an antigen
What describes a lymphoma?
Lymphomas involve lymphocytes and typically present in lymph nodes
