Evaluation of Erythrocytes Flashcards
What is the male and female reference value for RBCs?
4-6 Million/Cumm
What is the male and female reference value for Retics?
.5 to 1.5%
What is the male and female reference value for Hemoglobin?
Male: 16 +/- 2 or 14-18 g/dL
Female: 14 +/- 2 or 12-16 g/dL
What is the male and female reference value for Microhematocrit?
Male: 42-54%
Female: 36-48%
What is the male and female reference value for Erythrocyte Sedimentation Rate (ESR)?
Male: 0-20mm
Female: 0-15mm
What is the formula for manual RBC count and VF? What are the dimensions of the whole square field and 25 inside one square?
# of cells * Dilution Factor * Volume Factor = RBC count VF = Length * Width * Depth 1 square = 1x1mm 1 of 25 inside = .2x.2mm Depth = .1mm always
What type of stain is used in retic counts?
Supervital Stain
What are two common supervital stains?
New Methylene Blue
Brilliant Cresyl Blue
What form of hemoglobin cannot be measure by cyanmethemoglobin method?
Sulfhemoglobin
What are the 4 causes for elevated hemoglobin measurements?
- ) High WBC Count (>200,000)
- ) High Platelet Count (>700,000)
- ) Lipemia
- ) RBCs resistant to lysis
What is the rule of 3?
RBC * 3 ~ HGB
HGB * 3 = HCT +/- 3
What are the causes for hemoglobin and hematocrit not matching?
- ) High WBC
- ) High Platelets
- ) Lipemia
- ) Poik
How does under-centrifugation affect microhematocrit?
Increases
How does a decrease in blood volume (short draw) affect microhematocrit?
Decreases
How does a EDTA blood tube sitting out overnight at room temperature affect microhematocrit?
Increases
How does including the buffy coat affect microhematocrit?
Increases
What are the three stages and time lengths of Etythrocyte Sedimentation Rate (ESR).
- ) Rouleaux ~ 10 min
- ) Rapid Fall ~ 40 min
- ) Packing ~ 10 min
What plasma protein has the greatest impact on ESR?
Fibrinogen
How does placement next to a centrifuge affect ESR reading?
Increases
How does a decrease in blood volume (short draw) affect ESR reading?
Decreases
How does a EDTA blood tube sitting out overnight at room temperature affect ESR reading?
Decreases
How does the tube not being perpendicular affect ESR reading?
Increases
What is the formula and normal ranges for MCV?
HCT*10 / RBC
80-100 fL
What is the formula and normal ranges for MCH?
HGB*10 / RBC
27-31 pg