Flashcards HC8 (H10)
Functie real-time qPCR (engels)
To calculate the amount of specific segment of DNA in a particular sample.
What does the treshold line in rt-PCR represent?
It shows where the intensity of fluorescene in PCR reaches a treshold level beyond the background level
What does a low Ct value mean?
The sample started out with relatively more DNA
In the first step of dideoxy sequencing, what is annealed to the single stranded DNA to be sequenced?
Labeled oligonucleotide primer
Where do ddNTP’s differ from dNTP?
3’ carbon
What causes the termination of a polymerization reaction during Sanger’s sequencing?
Incorporation of ddNTP
During Sanger’s sequencing, ddNTPs are added at a concentration that is…
lower than dNTP
During Sanger’s sequencing, gel electrophoresis is used to
seperate terminated segments by size
Why is the design of single guided RNA limited to sequences that are next to PAMs?
Cas9 recognizes PAM sites to create double strand breaks
What enzyme is introduced into a cell along with a single guided RNA?
Cas9
What repair mechanism allows for the generation of precise mutations?
Homologous recombination
Four tubes are shown in the dideoxy sequencing reactions. What component is different for these four tubes?
Each tube has a different ddNTP
A ddNTP is a chain terminator because it is incorporated opposite a … in the growing DNA chain, but lack a …
Cytosine
3’-OH
Which is NOT a function of SDS during SDS-PAGE?
Denaturation of RNA
After SDS-PAGE, the gel is placed next to nitrocellulose. Where will the + charge need to be to get the proteins to migrate to the nitrocellulose. Why is nitrocellulose used?
Next to the nitrocellulose and nitrocellulose is used, because it binds proteins.
