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Fixatives Flashcards

1
Q

Maintains color - Museum fixative

A

10% Formol Saline

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2
Q

Recommended for routine post-mortem material

A

Formol Sublimate

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3
Q

Recommended for lipid fixation

A

Formol Calcium; Lillie; 1965

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4
Q

Used for glycogen

A

Genre’s fluid

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5
Q

Used in electron microscopy, fixing material for ultrathin sections for electron microscopy

A

Osmium tetroxide

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6
Q

Metallic fixatives:

A

Mercuric fixatives
Chromate fixatives
Lead fixatives

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7
Q

Ions of this acts by combining with acidic (carboxyl -COOH) groups of proteins and form especially strong combination with the sulfur (thiol) radicals

A

Mercuric Fixatives

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8
Q

Mercuric Fixatives:

A

Zenker’s
Zenker-Formol (Helly’s Sol’n)
Heidenhain’s Susa Fixtives
Schaudinn’s sublimated alcohol
B5 fixative

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9
Q

Best for fixation of blood forming or blood containing tissues i.e. pituitary gland, bone marrow, spleen, and liver

A

Zenker-Formol (Helly’s Solution)

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10
Q

Recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei

A

Zenker’s fluid

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11
Q

Viral inclusions (Negri bodies)

A

Zenker’s Fluid

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12
Q

Recommended for tumor biopsies esp. of the skin

A

Heidenhain’s Susa Solution

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13
Q

Commonly used for Bone marrow samples

A

B-5 Fixatives

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14
Q

Principles of this leads to disruption of the internal salt linkages of the protein increasing the reactive basic groups and thereby enhancing acidophilic in staining

A

Chromate fixatives

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15
Q

Chromate fixatives:

A

Chromic acid
Potassium dichromate
Regaud’s (Moller’s fluid)
Orth’s fluid

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16
Q

Preserves carbohydrates, precipitates proteins

A

Chromic acid

17
Q

Preserves mitochondria

A

Potassium dichromate

18
Q

used for mitochondria, mitotic figures and golgi bodies

A

Regaud’s (Moller’s fluid)

19
Q

demonstrates ricketssiae, tissue necrosis

A

Orth’s fluid

20
Q

Mainly used for mucopolysaccharides

A

Lead fixatives

21
Q

Picric Acid fixatives:

A

Bouin’s fluid
Brasil’s alcoholic - picro formol fixative

22
Q

For fixation of embryos and pituitary biopsies

A

Bouin’s fluid

23
Q

Rapidly denatures proteins by destroying hydrogen bonds

A

Alcoholic fixatives

24
Q

Alcoholic Fixatives:

A

Methyl alcohol
Ethyl alcohol
Isopropyl alcohol
Carnoy’s fluid
Newcomer’s fluid

25
Q

Excellent for fixing dry and wet smears, blood smears and bone marrow

A

Methyl Alcohol

26
Q

For touch preparation

A

Isopropyl alcohol

27
Q

Used in histochemistry for enzyme studies

A

Ethyl alcohol

28
Q

Recommended for fixing chromosomes and for urgent biopsies

A

Carnoy’s fluid

29
Q

Considered to be the most rapid fixative

A

Carnoy’s fluid

30
Q

Used in heat fixation

A

Ether saline (0.85%) pr 10% formal saline used

Aaaa (21 decks)

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