First Aid Microbiology Flashcards
mycoplasma unusual cell wall contains
sterols
mycobacteria unusual cell wall contains
mycolic acid, high lipid content
Which bugs do not stain well?
These Microbes May Lack Real Color Treponema (too thin) Mycobacteria (high lipid content) Mycoplasma (no cell wall) Legionella (silver stain( Rickettsia Chlymadia
Giemsa stain?
Certain Bugs Really Try my Patience Chlymadia Borrelia Rickettsiae Trypanosomes Plasmodium
PAS stain?
PASs the Sugar
Stains glycogen
Whipple diesase
Ziehl Neelson
Acid fast! Nocardia Mycobacterium
India ink stain
Cryptococcus neoformans
Silver stain
Fungi, H. Pylori, Legionella
Media used for H. Influenza?
Chocolate with factors X and V
Neisseria gonorrhea and meningitidis media?
thayer-martin (or VPN) stain. “Use VPN to connect to Neisseria”
Bordet-Gengou (potato) agar used for?
B. pertussus (Bordet for Bordetella)
EMB agar with green metallic sheen colonies?
E.Coli
Lactose fermenting turns what color of MacConkey?
PINK
Charcoal yeast extract
Legionella
Fungi culture?
Sab’s a fun guy!
Obligate aerobes bacteria?
Nagging Pests Must Breathe
Nocardia
P. aeruginosa (seen in wounds!)
MycoBacterium tuberculosis
Obligate anaerobes bacteria?
Anaerobes Can’t Breathe Air
Clostridium
Bacteroides
Actinomyces
Which antibiotics are not effective against anaerobes?
Aminoglycosides bc they need oxygen to enter bacterial cell wall
Obligate anaerobes lack what?
superoxide dismutase and catalase
Encapsulated bacteria? Capsules serve as antiphagocytic
SHiNE SKiS Strep pneumo H. influenza Neisseria mening E. Coli Salmonella Klebsiella group b STREP
Urease positive
CHuck norris hates PUNKSS Cryptococcus H.pylori Proteus Ureaplasma Nocardia Klebsiella S. epidermis S. sapro
sulfur granules
actinomyces
blue-green pigment
Psudeomonas aeruginosa
Red?
serratia
S. aureus protein A works how?
prevents opsonization/phagocytosis
M protein works how?
prevents phagocytosis. Expressed by group A strepp
bull neck
cornyebacterium
Mechanism of shiga toxin?
inactivates 60S ribosome by removing adenine from rRNA
cornyebacterium and pseudomonas mech?
inactivated EF2
Clostridium tetani and botulism mech?
cleaves SNARE proteins needed for neurotransmitter release
Clostridium tetani vs. botulism?
tetani: inhibitory, lockjaw
botulism: stimulatory, flaccid paralysis
gas gangrene and hemolysis on blood agar “double zone” hemolysis
Clostridium perfringens
strep pyogenes toxin?
streptolysin O and exotoxin A
endotoxin symptoms
Edema NO DIC/Death Outer membrane TNF-alpha O-antigen eXremely heat stable IL-1 Neyutrophil chemotaxis
anterior nares
S. aureus
TSST01 and scalded skin
S. aureus
How does TSST work?
superantigen, binds to MHC II and T cell receptors, resulting in polyclonal T activation
Strep pneumo symptoms?
MOPS Meningitis Otitis media pneumo sinusitis
rheumatic fever caused by?
strep pyogenes
rheumatic fever criteria?
JONES Joints Carditis Nodules Erythema marginatum Sydenham chorea
pseudomembranous pharyngitis
grayish white membrane cornyebacterium diptheraie
diptheria toxin mech?
prevents protein synthesis ADP ribosylation of EF2
dipicolonic acid?
spores
C. diff has which 2 toxins?
toxin a and b
only bacterium with polypeptide capsule?
anthrax. contains D-glutamate
only gram positive organism that produces LPS?
listeria
“rocket tails” that help move into cell membrane, avoiding antibody
listeria
Differeces between actinomyces and nocardia... Acid fast? Found where? Symptoms? Treatment?
Actinomyces are not acid fast. Norcardia is.
Actinomyces in normal oral flora. Nocardia in soil.
Actinomyces sulfur granules. Nocardia is pulmonary infections
Actinomyces treat with pen. Nocardia treat with sulfonamides
Ghon complex?
primary mycobacterium Tb