Fingerprinting Review Questions Flashcards
What is gel electrophoresis?
Electrolysis is how we push DNA in to the gel filter. This technology that separates the molecules that form DNA. it’s a way to visualize it.
Know the specific steps of gel electrophoresis. The steps you performed in class.
First step was practicing the micro pipe at and getting checked.
The first step of Joe lecture for Isis is setting up . Locate and label the sample tupes with the matching colors it says in the book. Aliquot 10 microliters of DNA sample. For each of the tubes you use a different tip eject it. Then you do the same with the restriction enzyme. Color coated and the same for each time change the tip and ejected it. Close and centrifuge. we powered the central fuse on and off five times. we took him to the water bath for 45 minutes, then handed them at the end of that to the teacher.. we made the gel. On the gel tray, tear off shift the tape on both sides, and secure it. Place the wall combs. Pour the Argelis gel to where there is a space between each comb, then let it cool down and put it away.
How is DNA able to travel through a gel?
Argo pectin in the gel interferes the movement of molecules, so it starts moving.
Which strands will travel further?
The short fragments travel the fastest versus the larger ones
What is the gel composed of? What is the preferred medium for running gel electrophoresis?
agarose is a polysaccharide extracted from the agar that is found in red algae or seaweed, the agarose gel is the preferred medium.
How does the gel sort DNA?
Agaropectin
What is a restriction enzyme? How does a restriction enzyme work? What are restriction enzymes composed of?
Restriction enzymes are proteins that cut DNA. oh restriction enzymes work is in the DNA. It cuts out the same codon set every human has and just singles out the unique ones. Restriction enzymes were cut from invading bacteria into harmless pieces.
Know all equipment that was used for the fingerprinting lab and be able to identify and label.
micropipette
tips
centrifuge
gel tray
argose gel
electrophoresis gel chamber
power pac
Is DNA slightly positive or negative?
slightly negative
What is the result of gel electrophoresis?
Results in distinct banding patterns.
Do you use a new micropipette tip every time you make a transfer?
yes, for p10 use the clear tip, for p20 use in the yellow tip.
What type of solution is used to run gel electrophoresis?
Buffer
What is a real world application for gel electrophoresis?
it’s used in forensics for profiling, and to identify.
