finals (questions to go over still) Flashcards
requirements of a chemical hygiene plan
- Eye wash
- Fire extinguisher
- Spill cleanup kits
- Biohazard disposal containers
- Protective gloves
mechanisms for minimizing exposure to hazard
- proper container labeling
- safety data sheets
- secondary labels
- blood-borne pathogen standard
in-house equipment
● Microscope
● Refractometer
● Microhematocrit centrifuge
● Clinical centrifuge
horizontal vs angle head centrifuge
angle:
● Higher speeds than swing arm
● Usually only one tube size
refractive index
Bending of light rays as they pass through from one medium to another with a different optical density
diaphragm
opens and closes for light control
rheostat
brightness control
condensor
focuses the light by raising and lowering
steps to cleaning microscope
hematopoiesis
production of blood cells and platelets
prenatal to adult hematopoiesis
Prenatal: Liver, spleen, thymus, red bone marrow
Neonatal and Juvenile: Red bone marrow
Adult: Primarily red bone marrow - If stressed, may revert to production in liver and spleen
Erythropoietin
Cytokine responsible for production of RBCs; Produced by cells in kidneys in response to decrease in oxygen tension in blood
lipemic causes what
false increase in total protein test
we do not perform a blood smear to estimate what
RBC numbers
normal canine RBC
biconcave disc shape with an area of central pallor
normal feline RBC
Round with little to no area of central pallor
Schistocytes
RBC fragments; half of cell is gone
Acanthocytes
Spur cells
Echinocytes
Burr cells (artifact)
Drepanocytes
sickle cells
Keratocytes
helment, blister cells
Spherocytes
Small, dark staining RBCs with reduced or no central pallor
Leptocytes
target cells
Stomatocytes
folded, white line (artifact)
Knizocytes
Barr cells; dark line
Elliptocytes
ovalcytes
Eccentrocytes
darker to one side; diabetes
Dacryocytes
tear drop (artifact if all face the same way)
Howell jolly bodies
response to anemia
cells of erythrocyte maturation series in order
rubriblast, prorubricyte, rubricyte, metarubricyte, reticulocyte, erythrocyte
how much sample for fecal float
1 teaspoon/ 2 grams
Spectrophotometers
Designed to measure the amount of light transmitted through a solution
supernatant
liquid components of sample after spinning
parfocal
don’t have to adjust course focus when moving from lower objectives to higher objectives