finals (questions to go over still) Flashcards
requirements of a chemical hygiene plan
- Eye wash
- Fire extinguisher
- Spill cleanup kits
- Biohazard disposal containers
- Protective gloves
mechanisms for minimizing exposure to hazard
- proper container labeling
- safety data sheets
- secondary labels
- blood-borne pathogen standard
in-house equipment
● Microscope
● Refractometer
● Microhematocrit centrifuge
● Clinical centrifuge
horizontal vs angle head centrifuge
angle:
● Higher speeds than swing arm
● Usually only one tube size
refractive index
Bending of light rays as they pass through from one medium to another with a different optical density
diaphragm
opens and closes for light control
rheostat
brightness control
condensor
focuses the light by raising and lowering
steps to cleaning microscope
hematopoiesis
production of blood cells and platelets
prenatal to adult hematopoiesis
Prenatal: Liver, spleen, thymus, red bone marrow
Neonatal and Juvenile: Red bone marrow
Adult: Primarily red bone marrow - If stressed, may revert to production in liver and spleen
Erythropoietin
Cytokine responsible for production of RBCs; Produced by cells in kidneys in response to decrease in oxygen tension in blood
lipemic causes what
false increase in total protein test
we do not perform a blood smear to estimate what
RBC numbers
normal canine RBC
biconcave disc shape with an area of central pallor
normal feline RBC
Round with little to no area of central pallor
Schistocytes
RBC fragments; half of cell is gone
Acanthocytes
Spur cells
Echinocytes
Burr cells (artifact)
Drepanocytes
sickle cells
Keratocytes
helment, blister cells
Spherocytes
Small, dark staining RBCs with reduced or no central pallor
Leptocytes
target cells
Stomatocytes
folded, white line (artifact)
Knizocytes
Barr cells; dark line
Elliptocytes
ovalcytes
Eccentrocytes
darker to one side; diabetes
Dacryocytes
tear drop (artifact if all face the same way)
Howell jolly bodies
response to anemia
cells of erythrocyte maturation series in order
rubriblast, prorubricyte, rubricyte, metarubricyte, reticulocyte, erythrocyte
how much sample for fecal float
1 teaspoon/ 2 grams
Spectrophotometers
Designed to measure the amount of light transmitted through a solution
supernatant
liquid components of sample after spinning
parfocal
don’t have to adjust course focus when moving from lower objectives to higher objectives
left-shift
increased number of immature neutrophils in blood
pancytopenia
decreased number of all cell types
CBC tests
polycythemia
dehrydrated
begin blood smear evaluation by scanning for
platelet clumps
large abnormal cells
microfilaria
5 abnormalities commonly missed
nRBCs
toxic granulation
platelet clumps
target cells
hemoparasites
pelger-huet anomaly
nuclear hyposegmentation
eosinophils and basophils
modulation and mediation
toxic cytoplasm changes in neutrophils
basophilic granules
dohle bodies
azurophilic granules
gigantism
foamy cytoplasm
reactive lymph
immunocyte
hyper segmentation is due to
aging
hemolytic anemia
siderotic granules
toxic cytoplasm changes are due to
inflammation
infection
drug toxicity
pyknosis
condensing nucleus as cell dies
karolysis
dissolution of nuclear membrane
karyorrhexis
fragmentation of nucleus after cell death
what to check on blood smear
width
length
shape
monolayer
ruptured leukocytes
smudge or basket cells
megathrombocytes
caused by early release of platelets
characteristics of each immature RBC
older cell type creates
more immature cells; hemorrhage or premature RBC destruction occurs
presence of rubricytes means
presence of metarubricytes and polychromatophils
immature thrombocytes from least to most
megakaryoblast
promegakaryocyte
megakaryocyte
proliferation pool (cells capable of mitosis)
myeoblasts
promyelocytes
myelocytes
maturation pool
band cells and metamyelocytes
characteristics
where do juvenile b lymphs mature
bone marrow
where do t lymphs mature
thymus
where do NK cells mature
bone marrow, thymus, and lymphoid tissue
granules of WBCs first appear in what stage
myelocyte stage
areas on your body that can be infected by parasites
oral cavity
esophagus
stomach
small and large intestine
life stages
egg
oocysts
larvae
segments
adults
fixed head centrifuge
more sensitive and takes less time
swinging head centrifuge
apply coverslip during centrifugation
use fecal sedimentation when
ova is too large
cellophane tape is used for
pinworm and tapeworm segments
direct smear for
giardia and diarrhea
modifies mcmasters technique
estimate of total eggs per gram
antrifugation
removes excess debris and takes less time
occult blood test
intestinal bleeding and hemoglobin
disadvantages to impedance
variation in cell size
morphological abnormalities
platelet clumping
nRBCs
manual cell counts on
avian and exotic tests
endpoint readings
reaction between sample and reagent
electrochemical method
electrodes with biosensor reagent strips
positive controls
comes with a result sheet
quality control
quality beads
hemolysis contributes to erroneous blood results
AST and ALT can elevate results
pancreatic function tests
lipase, blood glucose, amylase
liver function test
Serum bilirubin, Gamma Glutzmyl Transpeptidase (gamma GT), AST, ALT, Alkaline Phosphatase
kidney function tests
creatinine, BUN
skeletal and cardiac muscle function tests
sodium, potassium, phosphorous, magnesium, chloride, calcium, bicarbonate
protein function tests
fibrinogen, globulin, albumin