Finals - Component prep, therapy Flashcards
which of the ff is not a sterilization method used for blood?
a. Gamma irradiation
b. UV light exposure
c. Ethylene oxide
d. Electron beam
B
___ describe the characteristics of an ideal blood container (8)
1 Free from pyrogens, 2 Un-colored and transparent, 3 Identified by a lot number 4 not past expiration date, 5 should withstand sterilization methods 6 allow O2 and CO2 exchange 7 prevents water evaporation 8 may be latex free
(T/F) Blood bags with in-line filter can remove
leukocytes from whole blood and RBCs
true
____ are fever causing agent
pyrogens
*(T/F) Anticoagulant containing citrate can cause Ca2+ deficiencies in patients
true
coz citrate bind Ca2+
which of the ff anticoagulants are more commonly used?
a. Acid-Citrate-Dextrose (ACD)
b. Citrate-Phosphate-Dextrose (CPD)
c. Citrate-Phosphate-Dextrose-Dextrose (CP2D)
d. Citrate-Phosphate-Dextrose-Adenine (CPDA-1
C and D
(T/F) adenine component in Anticoagulant solutions decreases its shelf life
false
increases shelf life
____ volume of anticoagulant used for every 450 and 560 ml of blood
500ml = 70 ml anticoag 450ml = 63 ml anticoag
____ these solutions are added to blood bags for the survival of RBCs and increases exp date
additive solutions (AS)
___ the enzyme maintained by AS during the storage of blood
2,3-DPG
clarify this
____ the max amount of blood to be collected
10.5 mg blood/ Kg
____ for which transfusion therapy can a unit of blood be used for? (4)
1 RBCs
2 Platelets
3 Fresh Frozen Plasma (FFP)
4 Cryoprecipitate
which of the ff is false?
a. platelets rich plasma can be extracted from slow spinning of whole blood
b. FFP must be frozen w/in 6 hours after a hard sprin
c. Cryosuperprecipt is extracted before FFP is frozen
d. PRBC can be separated from both slow and hard spin
C
Cryo is extracted after slow-hard spin of thawed FFP
(T/F) usually whole blood is used in transfusion
false
WB is separated into components, allow transfusion therapy to be
targeted
(T/F) platelets are stored in RT
true
the volume used for the transfusion for neonates
a. 5 ml
b. 20- 25 ml
c. 30 ml
d. 10-25 mL
D
___ the type of blood bag used to store blood for neonatal transfusion
quad pack, pedi-packs
(T/F) when blood for neonate transfusion is done in sterile conditions (closed systems), the expiration date of the sample decreases
false
same as in original bag
which of the ff anticoagulants are more commonly used for NEONATES?
a. Acid-Citrate-Dextrose (ACD)
b. Citrate-Phosphate-Dextrose (CPD)
c. Citrate-Phosphate-Dextrose-Dextrose (CP2D)
d. Citrate-Phosphate-Dextrose-Adenine (CPDA-1)
D
esp for large volumes
which of the ff is NOT qualified for transfusion of aliquot/split units of blood?
a. Patients with severe chronic anemia
b. Overt heart failure
c. Severe renal failure
d. neonates
all are candidates :)
*____ the purpose of irradiation
Prevent the proliferation of T-lymphocytes = stop t-GVHD
_____ the phenomena where donor T cells attacks the recepients tissues
t-GVHD = transfusion-associated-graw versus host disease
___ list who are at risk for t-GVHD (5)
Immunocompromised patients
Bone marrow or stem cell transplant
Intrauterine transfusion of at risk fetuses
Patients who received blood from relatives
Patients receiving HLA-matched platelets
(T/F) the minimum dose of dose of gamma irradiation is > 5000cGY
false
25Gy (2500cGY)
– Cesium 137
– Cobalt 60
____ exp date of RBCS from the time of irradiation
28 days after
____ Absolute WBC count in the unit (Leukocyte reduced RBCs)
which pro inflammatory agents are prevented from being released by WBC in storage after sample is irradiated/ leukocyte reduced?
a. IL-1
b. IL-6
c. C3a
d. C5a
all of the above :)
(T/F) WBCs can also be removed at the bedside before transfusion
true
Reactions due to anti leukocyte antibodies
(present in the patient’s plasma) are prevented
(T/F) in the bedside removal of WBCs, biologic response modifiers and pro inflammatory agents are removed
false
___ list the adverse reactions WBCs are associated with (5)
Febrile-non-hemoly,c transfusion reactions,
Transfusion-related acute lung injury
transmission of EBV and CMV and HTLV
____ Frozen Deglycerolized RBCs
Method of freezing RBCs with glycerol
can be stored up to 10 yrs
(T/F) RBCs should be frozen with glycerol within 2 weeks of collection if CPD or CPDA-1 is used
first part False
stored w/in 6 days
AS-1, AS-3 and AS-5 – up to 42 days
(T/F) freezing RBC with glycerol prevents water from leaving the cell as ice is formed outside
true
uses 40% glycerol, stored at -80C
____ Deglycerolized RBCs expiration time
after 24hrs of thawing
which of the ff is not included: deglycerolized RBC products are free of
a. additives
b. WBCs
c. Platelets
d. Plasma
A
(T/F) platelets can be extracted from whole blood by either slow or hard speed spinning
true
see lec 9 notes pg 43 onwards
(T/F) a single whole blood should contain a therapeutic dose of 5 x 10^10 platelets
first part false
dose is from a pool of 5-6 RDP
____ the increase in the patient’s platelet count after a dose of platelet therapy
50,000/μL
____ when Platelet fraction is collected from a single donor (SDP) and remaining
components are returned to donor
apharesis platelet
(T/F) SDP contains a minimum of 3 x 10^11 platelets for one therapeutic dose
true
(T/F) irradiation of platelets affects its shelf life
false
which of the ff is not true about FFP?
a. obtained from SDP
b. contains FVIII
c. volume must be 500 ml
d. frozen w/in 6-8 hrs depending on anticoagulant
C
Volume 150 – 250 mL
(T/F) FFP from SDP is given to emergency patients requiring volume
true
which of the ff is not true about FFP?
a. stored at −18⁰C or colder
b. Expira,on – 1 year from date of collection
c. PF24 & FFP – thawed at 37⁰C
d. can be thawed in microwave
all is true :)
(T/F)>24 hrs – converted to “thawed plasma” and can
be used 3-5 days
true
___ are plasma that are deficient in FVIII/fibrinogen; PPP and used as volume expanders
Plasma and Liquid Plasma
___ plasma containing pools of up to 2500 units of ABO type-specific, triton X-100 and inactivates lipid-envelope virus e.g.,
HIV and HBV
Solvent Detergent Plasma (S/D plasma)
____ contains 80 units antihemophilic factor, cold precipt of FVIII, obtained from single whole blood unit collected in CPD or CPDA-1
Cryoprecipitate
which does not apply to cryo?
a. Expiration – 1 year if stored at ≥ −18⁰C
b. Transfused within 6 hrs of thawing
c. Adult dose – pool of 20 units
d. transfused w/in 4 hrs awer pooling
C
pool of 8-10 units
