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BBLec Sumaya > Finals: BLOOD PRESERVATION, COMPONENT PREPARATION AND STORAGE, ISSUANCE OF BLOOD, AND TRANSFUSION THERAPY > Flashcards

Finals: BLOOD PRESERVATION, COMPONENT PREPARATION AND STORAGE, ISSUANCE OF BLOOD, AND TRANSFUSION THERAPY Flashcards

1
Q

Indications of Washed RBcs

A

Increase mass RBCs of symptomatic anemic px with transfusion history of

Allergic reaction, Urticarial reaction
Anaphylactic reaction
Febrile non hemolytic reaction

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2
Q

This is not a substitute for leukocyte reduced rbcs

A

Washed RBcs

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3
Q

May also be used in infant or intrauterine transfusion

A

Washed Rbcs

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4
Q

How many percent of rbcs lost in the process after washing with saline?

A

10-20%

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5
Q

Storage and shelf life of Washed RBcs

A

Storage: 1-6 *C
Shelflife: 24 hrs open system

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6
Q

Indications of Platelet Concentrate

A

Active bleeding px who are thrombocytopenic (<20,000 /uL)
Cancer px dueto chemotheraphy and radiation
Thrombocytopenic pre operative px (<50,000/uL)
Dengue hemorrhagic fever

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7
Q

Preparation of RDP

A

Prepared from whole blood (1 unit)
• Prepared within 6 hours after collection
• Whole blood at room temp→ 1st step: light spin (3200 rpm),
2-3min→ produce platelet rich plasma separated from red
cells→ 2nd step: heavy spin (3600 rpm), 5 min→ platelets
separate from plasma→ remove plasma into another bag,
leaving 50 – 70 mL for the platelets.

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8
Q

How many platelets must contain in RDP?

A

> 5.5x10^10

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9
Q

How many plasma must contain in RDP and pH

A

40-70 mL plasma, pH >6.2

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10
Q

RDP may be pooled for how many units?

A

4-6/8 units

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11
Q

RDP new method is also called

A

Buffy Coat Method

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12
Q

In RDP, 1 unit should increase platelet by

A

5,000-10,000/ uL

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13
Q

Interpretation of the Increment for Platelet should be done:

A

After 1 hour of post transfusion

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14
Q

Good increment is =

A

> 10,000/uL

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15
Q

Refractoriness=

A

<5,000/uL

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16
Q

Reason why refractoriness <5,000/uL

A

Unresponsive of platelet transfusion due to HLA/HPA, DIC, Sepsis and fever

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17
Q

Preparation of SDP

A

Apheresis

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18
Q

SDP must contain how many platelets

A

3x10^11 equivalent to 4-6 units

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19
Q

SDP must contain how many mL of plasma?

A

200-400mL

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20
Q

Indications of SDP

A

Indicated for patients with Idiopathic Thrombocytopenic Purpura

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21
Q

In SDP, 1 unit should increase platelets by

A

30,000-60,000/uL in 75 kg px

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22
Q

Storage and Shelflife of SDP

A

Storage: 20-24 *C
Shelf Life: 5days with constant agitation

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23
Q

Leukocyte Reduce RBcs indications

A

Can be use for ITP

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24
Q

In Leukocyte Reduced RBCs, how many leukocytes must contain in a whole blood

A

<8.3x10^5

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25
Q

In Leukocyte Reduced RBCs, how many leukocytes must contain in a pheresis

A

<5x10^6

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26
Q

Storage and Shelflife of LRP

A

Storage: 20-24 *C
Shelf life:
Open System: 4hrs
Apheresis: 5 days with constant agitation

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27
Q

Preparation of Pre Storage pooled Platelets

A

4-6 ABO identical platelet pooled using closed system

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28
Q

Indications of Pre Storage Pooled Platelets

A

Severe thrombocytopenia and Abnormal Platelet Function

29
Q

Storage and Shelflife of Pre Storage Pooled Platelets

A

Storage: 20-24 *C
Shelflife: Extend outdate due to close system

30
Q

Preparation of Apheresis Granulocytes

A

Uses hydroxyethyl starch as a sedimenting agent, with the administration of Corticosteroids to the donor 12-24hrs before donation

31
Q

In Apheresis Granulocytes,
1 unit must contain; how many mL of RBCs

A

> 1.0x10^10 ; 20-50mL

32
Q

In Apheresis Granulocytes, donor should have how many uL of WBCs

A

4x10^9 /uL WBCs

33
Q

Indications of Apheresis Granulocytes

A

Px with absolute granulocyte count of <350-500/ uL
Px with granulocyte dysfunction
Myeloid hypoplasia
Px who are unresponsive to antibiotic
Severe neutropenia

34
Q

Storage & Shelflife of Apheresis Granulocytes

A

Storage: 20-24 *C without agitation
Shelf life: 24 hours

35
Q

In Fresh Frozen Plasma preparation

A

WB in CPD > process within 8 hours
WB in ACD > process within 6 hours
FFP- thaw in waterbath for 30-37*C in 30-45mins
Thawed plasma should be transfused immediately

36
Q

In Fresh Frozen Plasma, for labile factors store it at

A

1-6*C for 6 hours

37
Q

In Fresh Frozen Plasma, when Factor V is not needed store it at

A

4*C for up to 24hours

38
Q

Fresh Frozen Plasma contains

A

All coagulation factors

39
Q

Indications for PT

A

> 16secs

40
Q

Indications for aPTT

A

> 60secs

41
Q

FFP is need for bleeding px who requires factors

A

2,5,7,9,10

42
Q

Indication of FFP

A

Px with liver disease
Treatment of TTP
Antithrombin III deficiencies
Reverse effect of warfarin

43
Q

FFP storage and shelflife

A

Storage:
-18C for 1year
-65C for 7 years

44
Q

Plasma Frozen 24 hr–PF/FP24 is frozen within?

A

8-24 hours of collection

45
Q

Plasma Frozen 24 hr–PF/FP24 is stored at

A

-18*C

46
Q

Plasma Frozen 24 hr–PF/FP24 contains all

A

Stable proteins found in FFP

47
Q

Recovered Plasma and Liquid Plasma

A

Can be prepared directly from WB
By product of platelet concentrate
Cryoprecipitate production (Cryosupernate)

48
Q

Use of Recovered Plasma & Liquid Plasma

A

Use as volume expander
For the manufacturer of plasma fractionation products such as:
Plasma Protein Fraction
Normal Serum Albumin
Immune serum globulin

49
Q

Recovered Plasma and Liquid Plasma, storage and shelflife

A

-18*C or Colder for 5years

50
Q

Recovered Plasma and Liquid Plasma is labelled as

A

Plasma

51
Q

Recovered Plasma and Liquid Plasma, in liquid state, the product is labeled as and stored at

A

Liquid Plasma; 1-6*C

52
Q

Recovered Plasma and Liquid Plasma, can be transfused up to

A

5days after expiration of WB

53
Q

S/D-Pooled Plasma consist of pools not more than

A

25 units of ABO type-specific plasma

54
Q

S/D- Pooled Plasma, is treated with solvent/detergent, wherein solvent is? And Detergent is?

A

Solvent- Tri-n-butyl Alcohol
Detergent- Triton

55
Q

S/D- Pooled Plasma is treated with solvent and detergent in the thawing process to?

A

Inactivate lipid-enveloped viruses such as HIV and Hepatitis B

56
Q

S/D- Pooled Plasma contains

A

Stable and Labile clotting factors

57
Q

S/D- Pooled Plasma lacks

A

Willebrand’s factor multimers

58
Q

S/D- Pooled Plasma volume is

A

200mL

59
Q

It is the cooled precipitated concentration of Factor VIII

A

Cryoprecipitate

60
Q

Preparation of Cryoprecipitate

A

FFP thawed @1-6 *C from single WB (CPD/CPDA-1), by heavy spin centrifugation (5,000xg for 7mins)

61
Q

Cryoprecipitated anti hemophilic factor contains

A

Fibrinogen 150-250 mg
AHF VIII -120 IU
vWF 40-70%
F VIII 20-30%
Fibronectin

62
Q

Cryoprecipitated Anti hemophilic Factor

Before infusion: Frozen cryoprecipitate→ thaw at 30-37 Degrees
Celsius→ thawed cryoprecipitate
• Store at room temp and transfuse immediately or transfuse
within 6 hours

A
63
Q

Pooled cryoprecipitate: Transfuse within

A

4hrs (open system)

64
Q

Cryoprecipitated Anti-hemophilic Factor (AHF)
Indications:

A

Px with fibrinogen deficiency
Hemophilia A
Von Willebrand Disease
Factor XIII deficiency

65
Q

Cryoprecipitated Anti-hemophilic Factor (AHF)
is not indicated with TTP must use:

A

FFP OR CRYOSUPERNATE

66
Q

Cryoprecipitated Anti-hemophilic Factor (AHF)
Storage & Shelf life

A

Frozen -18*C for 1year
After thawing: RT
Shelf life: 1 year if frozen or
After thawing: Single units: 6 hours
Pooled units: 6 hours if closed system, if open: 4 hours

67
Q

Treatment for classic hemophilia and hemophilia A, and in deficiency of FVIII

A

Factor VIII Concentrate

68
Q

Factor VIII, When prepared from pooled plasma→3 techniques to destroy viral contaminants:

A

Pasteurization
• Solvent and detergent technique
• Monoclonal purification

Forms:
• Porcine Factor VIII
• Recombinant Factor VIII using D

BBLec Sumaya (6 decks)

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