Finals: BLOOD PRESERVATION, COMPONENT PREPARATION AND STORAGE, ISSUANCE OF BLOOD, AND TRANSFUSION THERAPY

Question 1

Indications of Washed RBcs

Answer 1

Increase mass RBCs of symptomatic anemic px with transfusion history of

Allergic reaction, Urticarial reaction
Anaphylactic reaction
Febrile non hemolytic reaction

Question 2

This is not a substitute for leukocyte reduced rbcs

Answer 2

Washed RBcs

Question 3

May also be used in infant or intrauterine transfusion

Answer 3

Washed Rbcs

Question 4

How many percent of rbcs lost in the process after washing with saline?

Answer 4

10-20%

Question 5

Storage and shelf life of Washed RBcs

Answer 5

Storage: 1-6 *C
Shelflife: 24 hrs open system

Question 6

Indications of Platelet Concentrate

Answer 6

Active bleeding px who are thrombocytopenic (<20,000 /uL)
Cancer px dueto chemotheraphy and radiation
Thrombocytopenic pre operative px (<50,000/uL)
Dengue hemorrhagic fever

Question 7

Preparation of RDP

Answer 7

Prepared from whole blood (1 unit)
• Prepared within 6 hours after collection
• Whole blood at room temp→ 1st step: light spin (3200 rpm),
2-3min→ produce platelet rich plasma separated from red
cells→ 2nd step: heavy spin (3600 rpm), 5 min→ platelets
separate from plasma→ remove plasma into another bag,
leaving 50 – 70 mL for the platelets.

Question 8

How many platelets must contain in RDP?

Answer 8

> 5.5x10^10

Question 9

How many plasma must contain in RDP and pH

Answer 9

40-70 mL plasma, pH >6.2

Question 10

RDP may be pooled for how many units?

Answer 10

4-6/8 units

Question 11

RDP new method is also called

Answer 11

Buffy Coat Method

Question 12

In RDP, 1 unit should increase platelet by

Answer 12

5,000-10,000/ uL

Question 13

Interpretation of the Increment for Platelet should be done:

Answer 13

After 1 hour of post transfusion

Question 14

Good increment is =

Answer 14

> 10,000/uL

Question 15

Refractoriness=

Answer 15

<5,000/uL

Question 16

Reason why refractoriness <5,000/uL

Answer 16

Unresponsive of platelet transfusion due to HLA/HPA, DIC, Sepsis and fever

Question 17

Preparation of SDP

Answer 17

Apheresis

Question 18

SDP must contain how many platelets

Answer 18

3x10^11 equivalent to 4-6 units

Question 19

SDP must contain how many mL of plasma?

Answer 19

200-400mL

Question 20

Indications of SDP

Answer 20

Indicated for patients with Idiopathic Thrombocytopenic Purpura

Question 21

In SDP, 1 unit should increase platelets by

Answer 21

30,000-60,000/uL in 75 kg px

Question 22

Storage and Shelflife of SDP

Answer 22

Storage: 20-24 *C
Shelf Life: 5days with constant agitation

Question 23

Leukocyte Reduce RBcs indications

Answer 23

Can be use for ITP

Question 24

In Leukocyte Reduced RBCs, how many leukocytes must contain in a whole blood

Answer 24

<8.3x10^5

Question 25

In Leukocyte Reduced RBCs, how many leukocytes must contain in a pheresis

Answer 25

<5x10^6

Question 26

Storage and Shelflife of LRP

Answer 26

Storage: 20-24 *C Shelf life: Open System: 4hrs Apheresis: 5 days with constant agitation

Question 27

Preparation of Pre Storage pooled Platelets

Answer 27

4-6 ABO identical platelet pooled using closed system

Question 28

Indications of Pre Storage Pooled Platelets

Answer 28

Severe thrombocytopenia and Abnormal Platelet Function

Question 29

Storage and Shelflife of Pre Storage Pooled Platelets

Answer 29

Storage: 20-24 *C Shelflife: Extend outdate due to close system

Question 30

Preparation of Apheresis Granulocytes

Answer 30

Uses hydroxyethyl starch as a sedimenting agent, with the administration of Corticosteroids to the donor 12-24hrs before donation

Question 31

In Apheresis Granulocytes, 1 unit must contain; how many mL of RBCs

Answer 31

>1.0x10^10 ; 20-50mL

Question 32

In Apheresis Granulocytes, donor should have how many uL of WBCs

Answer 32

4x10^9 /uL WBCs

Question 33

Indications of Apheresis Granulocytes

Answer 33

Px with absolute granulocyte count of <350-500/ uL Px with granulocyte dysfunction Myeloid hypoplasia Px who are unresponsive to antibiotic Severe neutropenia

Question 34

Storage & Shelflife of Apheresis Granulocytes

Answer 34

Storage: 20-24 *C without agitation Shelf life: 24 hours

Question 35

In Fresh Frozen Plasma preparation

Answer 35

WB in CPD > process within 8 hours WB in ACD > process within 6 hours FFP- thaw in waterbath for 30-37*C in 30-45mins Thawed plasma should be transfused immediately

Question 36

In Fresh Frozen Plasma, for labile factors store it at

Answer 36

1-6*C for 6 hours

Question 37

In Fresh Frozen Plasma, when Factor V is not needed store it at

Answer 37

4*C for up to 24hours

Question 38

Fresh Frozen Plasma contains

Answer 38

All coagulation factors

Question 39

Indications for PT

Answer 39

>16secs

Question 40

Indications for aPTT

Answer 40

>60secs

Question 41

FFP is need for bleeding px who requires factors

Answer 41

2,5,7,9,10

Question 42

Indication of FFP

Answer 42

Px with liver disease Treatment of TTP Antithrombin III deficiencies Reverse effect of warfarin

Question 43

FFP storage and shelflife

Answer 43

Storage: -18*C for 1year -65*C for 7 years

Question 44

Plasma Frozen 24 hr–PF/FP24 is frozen within?

Answer 44

8-24 hours of collection

Question 45

Plasma Frozen 24 hr–PF/FP24 is stored at

Answer 45

-18*C

Question 46

Plasma Frozen 24 hr–PF/FP24 contains all

Answer 46

Stable proteins found in FFP

Question 47

Recovered Plasma and Liquid Plasma

Answer 47

Can be prepared directly from WB By product of platelet concentrate Cryoprecipitate production (Cryosupernate)

Question 48

Use of Recovered Plasma & Liquid Plasma

Answer 48

Use as volume expander For the manufacturer of plasma fractionation products such as: Plasma Protein Fraction Normal Serum Albumin Immune serum globulin

Question 49

Recovered Plasma and Liquid Plasma, storage and shelflife

Answer 49

-18*C or Colder for 5years

Question 50

Recovered Plasma and Liquid Plasma is labelled as

Answer 50

Plasma

Question 51

Recovered Plasma and Liquid Plasma, in liquid state, the product is labeled as and stored at

Answer 51

Liquid Plasma; 1-6*C

Question 52

Recovered Plasma and Liquid Plasma, can be transfused up to

Answer 52

5days after expiration of WB

Question 53

S/D-Pooled Plasma consist of pools not more than

Answer 53

25 units of ABO type-specific plasma

Question 54

S/D- Pooled Plasma, is treated with solvent/detergent, wherein solvent is? And Detergent is?

Answer 54

Solvent- Tri-n-butyl Alcohol Detergent- Triton

Question 55

S/D- Pooled Plasma is treated with solvent and detergent in the thawing process to?

Answer 55

Inactivate lipid-enveloped viruses such as HIV and Hepatitis B

Question 56

S/D- Pooled Plasma contains

Answer 56

Stable and Labile clotting factors

Question 57

S/D- Pooled Plasma lacks

Answer 57

Willebrand’s factor multimers

Question 58

S/D- Pooled Plasma volume is

Answer 58

200mL

Question 59

It is the cooled precipitated concentration of Factor VIII

Answer 59

Cryoprecipitate

Question 60

Preparation of Cryoprecipitate

Answer 60

FFP thawed @1-6 *C from single WB (CPD/CPDA-1), by heavy spin centrifugation (5,000xg for 7mins)

Question 61

Cryoprecipitated anti hemophilic factor contains

Answer 61

Fibrinogen 150-250 mg AHF VIII -120 IU vWF 40-70% F VIII 20-30% Fibronectin

Question 62

Cryoprecipitated Anti hemophilic Factor Before infusion: Frozen cryoprecipitate→ thaw at 30-37 Degrees Celsius→ thawed cryoprecipitate • Store at room temp and transfuse immediately or transfuse within 6 hours

Question 63

Pooled cryoprecipitate: Transfuse within

Answer 63

4hrs (open system)

Question 64

Cryoprecipitated Anti-hemophilic Factor (AHF) Indications:

Answer 64

Px with fibrinogen deficiency Hemophilia A Von Willebrand Disease Factor XIII deficiency

Question 65

Cryoprecipitated Anti-hemophilic Factor (AHF) is not indicated with TTP must use:

Answer 65

FFP OR CRYOSUPERNATE

Question 66

Cryoprecipitated Anti-hemophilic Factor (AHF) Storage & Shelf life

Answer 66

Frozen -18*C for 1year After thawing: RT Shelf life: 1 year if frozen or After thawing: Single units: 6 hours Pooled units: 6 hours if closed system, if open: 4 hours

Question 67

Treatment for classic hemophilia and hemophilia A, and in deficiency of FVIII

Answer 67

Factor VIII Concentrate

Question 68

Factor VIII, When prepared from pooled plasma→3 techniques to destroy viral contaminants:

Answer 68

Pasteurization • Solvent and detergent technique • Monoclonal purification Forms: • Porcine Factor VIII • Recombinant Factor VIII using D