Final Review Flashcards

0
Q

For the following reaction,

A

E. May occur at some concentrations of substrate and product

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1
Q
Which of the following is an electron donor used for reductive biosynthesis?
A) NADH
B)NADPH
C) FADH2
D) CoA
E) ATP
A

B. NADPH

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2
Q

All of the following contribute to the large, negative, free-energy change upon hydrolysis of “high-energy” compounds except:
A) electrostatic repulsion in the reactant.
B) low activation energy of forward reaction.
C) stabilization of products by extra resonance forms.
D) stabilization of products by ionization.
E) stabilization of products by solvation

A

B. low activation energy of forward reaction.

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3
Q

Biological oxidation-reduction reactions never involve:
A) transfer of e- from one molecule to another.
B) formation of free e-.
C) transfer of H+ (or H3O+) from one molecule to another.
D) formation of free H+ (or H3O+).
E) none of the above.

A

B. formation of free e-.

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4
Q

What is the purpose of phosphorylating glucose in cytosol?
A) To trap glucose in the cell
B) To destabilize glucose and facilitate the next series of metabolic steps
C) To convert it to a more soluble form
D) All of the above
E) A and B only

A

E. A and B only

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5
Q

Glycolysis is the name given to a metabolic pathway occurring in many different cell types. It consists of 11 enzymatic steps that convert glucose to lactic acid. Glycolysis is an example of:
A) aerobic metabolism.
B) anabolic metabolism.
C) a net reductive process.
D) fermentation.
E) oxidative phosphorylation.

A

D. Fermentation

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6
Q

What is the function of a thioester intermediate such as the one formed from GAP?
A) It speeds up the actual reaction so that more product can be made.
B) The thioester shifts the equilibrium of the first stage of the reaction.
C) The thioester allows the two-step reaction to be coupled so the second reaction, the energetically unfavorable phosphorylation, can proceed.
D) The thioester intermediate induces a conformational change that alters the enzyme specificity.

A

C. The thioester allows the two-step reaction to be coupled so the second reaction, the energetically unfavorable phosphorylation, can proceed.

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7
Q

What is the additional metabolite that is required for the conversion of 3- phosphoglycerate to 2-phosphoglycerate?
A) 1-phosphoglycerate D) 2,3-bisphosphoglycerate
B) Diacylglycerol E) 1,3-bisphosphoglycerate
C) NADH
D) 2,3-bisphosphoglycerate
E) 1,3-bisphosphoglycerate

A

D. 2,3-bisphosphoglycerate

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8
Q

Fructose can enter glycolysis at two distinct points, depending on the tissue. How is fructose metabolized in adipose tissue?
A) Fructose is cleaved to two molecules of GAP.
B) Fructose is converted to fructose-1-phosphate.
C) Fructose is converted to fructose-6-phosphate.
D) Fructose is cleaved to GAP and DHAP.
E) Fructose is converted to glucose, which enters the pathway.

A

C. Fructose is converted to fructose-6-phosphate

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9
Q

During strenuous exercise, the NADH formed in the glyceraldehyde 3- phosphate dehydrogenase reaction in skeletal muscle must be reoxidized to NAD+ if glycolysis is to continue. The most important reaction involved in the reoxidation of NADH is:
A) dihydroxyacetone phosphate → glycerol 3-phosphate
B) glucose 6-phosphate → fructose 6-phosphate
C) isocitrate →α-ketoglutarate
D) oxaloacetate → malate
E) pyruvate → lactate

A

E. Pyruvate –> lactate

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10
Q

In an anaerobic muscle preparation, lactate formed from glucose labeled in
C-3 and C-4 would be labeled in:
A) all three carbon atoms.
B) only the carbon atom carrying the OH.
C) only the carboxyl carbon atom.
D) only the methyl carbon atom.
E) the methyl and carboxyl carbon atoms.

A

C. Only the carboxyl carbon atom

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11
Q

The first reaction in glycolysis that results in the formation of an energy-rich compound (i.e., a compound whose hydrolysis has a highly negative ΔG’°) is catalyzed by:
A) glyceraldehyde 3-phosphate dehydrogenase.
B) hexokinase.
C) phosphofructokinase-1.
D) phosphoglycerate kinase.
E) triose phosphate isomerase.

A

A. Glyceraldehyde-3-phosphate dehydrogenase

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12
Q

Gluconeogenesis must use “bypass reactions” to circumvent three reactions in the glycolytic pathway that are highly exergonic and essentially irreversible. Reactions carried out by which three of the enzymes listed must be bypassed in the gluconeogenic pathway?
1) Hexokinase
2) Phosphoglycerate kinase
3) Phosphofructokinase-1
4) Pyruvate kinase
5) Triosephosphate isomerase
A) 1,2,3
B) 1,2,4
C) 1,4,5
D) 1,3,4
E) 2,3,4

A

D. 1,3,4

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13
Q

Which of the following statements about gluconeogenesis in animal cells is true?
A) A rise in the cellular level of fructose-2,6-bisphosphate stimulates the rate of gluconeogenesis.
B) An animal fed a large excess of fat in the diet will convert any fat not needed for energy production into glycogen to be stored for later use.
C) The conversion of fructose 1,6-bisphosphate to fructose 6-phosphate is not catalyzed by phosphofructokinase-1, the enzyme involved in glycolysis.
D) The conversion of glucose 6-phosphate to glucose is catalyzed by hexokinase, the same enzyme involved in glycolysis.
E) The conversion of phosphoenol pyruvate to 2-phosphoglycerate occurs in two steps, including a carboxylation.

A

C. The conversion of fructose 1,6-bis phosphate to fructose 6-phosphate is not catalyzed by phosphofructokinase-1, the enzyme involved in glycolysis

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14
Q
1)​What family of enzymes is responsible for the first reaction of β-oxidation of fatty acids?
A)​Tranferases
B)​Dehydrogenases
C)​Translocases
D)​Thiolases
E)​Phosphatases
A

B. Dehydrogenases

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15
Q

2)​Which of the following choices is correct regarding the hormonal regulation of fatty acid synthesis?
A)​The binding of glucagon to its membrane receptors leads to the activation of a protein kinase which phosphorylates acetyl-CoA carboxylase, resulting in inhibition of fatty acid biosynthesis.
B)​The binding of glucagon to its membrane receptors leads to the activation of a phosphatase which dephosphorylates acetyl-CoA carboxylase, resulting in inhibition of fatty acid biosynthesis.
C)​The binding of glucagon to its membrane receptors leads to the activation of a protein kinase which phosphorylates acetyl-CoA carboxylase, resulting in activation of fatty acid biosynthesis.
D)​The binding of insulin to its membrane receptors, leads to the activation of protein kinases, which leads to phosphorylation of acetyl-CoA carboxylase, causing the activation of fatty acid biosynthesis.
E)​The binding of insulin to its membrane receptors, leads to the activation of phosphodiesterases, which leads to the dephosphorylation of acetyl-CoA carboxylase, causing the inhibition of fatty acid biosynthesis.

A

A. The binding of glucagon to its membrane receptors leads to the activation of a protein kinase that phosphorylates acetyl-CoA carboxylase, resulting in inhibition of fatty acid biosynthesis.

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16
Q
3. The glycerol produced from the hydrolysis of triacylglycerides enters glycolysis as:
A)​glucose.
B)​glucose-6-phosphate.
C)​dihydroxyacetone phosphate.
D)​pyruvate.
E)​glyceryl CoA.
A

C. Dihydroxyacetone phosphate

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17
Q
4. Saturated fatty acids are degraded by the stepwise  reactions of β oxidation, producing acetyl-CoA. Under aerobic conditions, how many ATP molecules would be produced as a consequence of removal of each acetyl-CoA?
A)​2
B)​3
C)​4
D)​5
E)​6
A

C. 4

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18
Q
  1. Which of the following statements concerning the β oxidation of fatty acids is true?
    A)​About 1,200 ATP molecules are ultimately produced per 20-carbon fatty acid oxidized.
    B)​One FADH2 and two NADH are produced for each acetyl-CoA.
    C)​The free fatty acid must be carboxylated in the β position by a biotin-dependent reaction before the process of β oxidation commences.
    D)​The free fatty acid must be converted to a thioester before the process of β oxidation commences.
    E)​Two NADH are produced for each acetyl-CoA.
A

D. The free fatty acid must be converted to a thioester before the process of B oxidation commences

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19
Q
6. If an aerobic organism (e.g., the bacterium E. coli) were fed each of the following four compounds as a source of energy, the energy yield per mole from these molecules would be in the order:
A)​alanine > glucose > palmitate (16:0)
B)​glucose > alanine > palmitate
C)​glucose > palmitate > alanine
D)​palmitate > alanine > glucose
E)​palmitate > glucose > alanine
A

E)​palmitate > glucose > alanine

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20
Q

Which of the following is not true of the reaction producing malonyl-CoA during fatty acid synthesis?
A)​It is stimulated by citrate.
B)​It requires acyl carrier protein (ACP).
C)​It requires CO2 (or bicarbonate).
D)​One mole of ATP is converted to ADP + Pi for each malonyl-CoA synthesized.
E)​The cofactor is biotin.

A

B. It requires acyl carrier protein (ACP)

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21
Q
  1. If malonyl-CoA is synthesized from 14CO2 and unlabeled acetyl-CoA, and the labeled malonate is then used for fatty acid synthesis, the final product (fatty acid) will have radioactive carbon in:
    A)​every C.
    B)​every even-numbered C-atom.
    C)​every odd-numbered C-atom.
    D)​no part of the molecule.
    E)​only the omega-carbon atom (farthest carbon from C-1).
A

D. No part of the molecule

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22
Q
  1. In comparing fatty acid biosynthesis with β oxidation of fatty acids, which of the following statements is incorrect?
    A)​A thioester derivative of crotonic acid (trans-2-butenoic acid) is an intermediate in the synthetic path, but not in the degradative path.
    B)​A thioester derivative of D-β-hydroxybutyrate is an intermediate in the synthetic path, not in the degradative path.
    C)​Fatty acid biosynthesis uses NADPH exclusively, whereas β oxidation uses NAD+ exclusively.
    D)​Fatty acid degradation is catalyzed by cytosolic enzymes; fatty acid synthesis by mitochondrial enzymes.
    E)​The condensation of two moles of acetyl-CoA in the presence of a crude extract is more rapid in bicarbonate buffer than in phosphate buffer at the same pH; the cleavage of acetoacetyl-CoA proceeds equally well in either buffer.
A

D. Fatty acid degradation is catalyzed by cytotoxic enzymes, fatty acid synthesis by mitochondrial enzymes

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23
Q

Which of the following is not an intermediate in the synthesis of lanosterol from acetyl-CoA?
A)​Isopentenyl pyrophosphate
B)​Malonyl-CoA
C)​Mevalonate
D)​Squalene
E)​β-Hydroxy-β-methylglutaryl-CoA (HMG-CoA)

A

B. Malonyl CoA

24
Q

Which of these statements about cholesterol synthesis is true?
A)​Cholesterol is the only known natural product whose biosynthesis involves isoprene units.
B)​Only half of the carbon atoms of cholesterol are derived from acetate.
C)​Squalene synthesis from farnesyl pyrophosphate results in the release of two moles of PPi for each mole of squalene formed.
D)​The activated intermediates in the pathway are CDP-derivatives.
E)​The condensation of two five-carbon units to yield geranyl pyrophosphate occurs in a “head-to-head” fashion.

A

C. Squalene synthesis from farnesyl pyrophosphate results in the release of two moles of PPi for each mole of Squalene formed.

25
Q

In amino acid catabolism, the first reaction for many amino acids is a(n):
A)​decarboxylation requiring thiamine pyrophosphate (TPP).
B)​hydroxylation requiring NADPH and O2.
C)​oxidative deamination requiring NAD+.
D)​reduction requiring pyridoxal phosphate (PLP).
E)​transamination requiring pyridoxal phosphate (PLP).

A

E. Transamination requiring pyridoxyl phosphate (PLP)

26
Q
Which of these directly donates a nitrogen atom for the formation of urea during the urea cycle?
A)​Adenine
B)​Aspartate
C)​Creatine
D)​Glutamate
E)​Ornithine
A

B. Aspartame

27
Q

What physiological conditions would promote the synthesis of fatty acids? (Choose all that apply).
A)​High ATP: ADP ratio
B)​High Insulin: Glucagon ratio
C)​Release of Glucagon and Epinephrine
D)​Accumulation of long chain acyl CoAs and other β-oxidation intermediates
E)​Accumulation of Citrate

A

A,B,E

28
Q

With regard to the binding of LDL cholesterol to the LDL receptor which of the following statements is FALSE?
A)​Oversupply of cholesterol stimulates the upregulation of LDL receptors
B)​Increased levels of serum cholesterol will lead to an upregulation of LDL receptors
C)​Lysosomes degrade the carrier protein LDL is attached to
D)​The LDL receptor is endocytosed when bound to LDL
E)​Oversupply of cholesterol activates ACAT
Short Answer/Long Answer

A

A. Oversupply of cholesterol stimulates the upregulation of LDL receptors

29
Q
What molecule must be excluded from the active site of glycogen phosphorylase?
A. Glucose
B. Glucose-1-phosphate
C. Water
D. All of the above. 
E. None of the above.
A

C. Water

30
Q

How is phosphorylase b converted into phosphorylase a?
A. Addition of a phosphate to a serene residue.
B. Dimerization, which forms the active site pocket
C. Cleavage of 10 amino acids from the N-terminal end of the protein
D. All of the above
E. None of the above.

A

A. Addition of a phosphate to a shrine residue

31
Q

Insulin in the bloodstream is a response to increased blood glucose, and
A. Stimulates gluconeogenesis
B. Inhibits glycolysis
C. Stimulates glycogen synthesis in the muscle and liver
D. Stimulates glycogen breakdown in the liver
E. Inhibits phosphorite in phosphatase-1

A

C. Stimulates glycogen synthesis in muscle and liver

32
Q

Which of the following is true of glycogen synthase?
A. Activation of the enzyme involves a phosphorylation
B. It catalyzed addition of glucose residues to the non-reducing end of a glycogen chain by formation of (alpha 1-4) bonds.
C. It uses glucose-6-phosphate as a donor of glucose units.
D. The conversion of an active to an inactive form of the enzyme is controlled by the concentration of cAMP.
E. The enzyme has measurable activity only in liver.

A

B. It catalyzed addition of glucose residues to the non-reducing end of a glycogen chain by formation of (alpha 1-4) bonds

33
Q
Glycogen phosphorylase a can be inhibited at an allosteric site by: 
A. AMP
B. calcium
C. GDP
D. glucagon
E. Glucose
A

E. Glucose

34
Q

Which of the following directly results in the activation of glycogen synthase?
A. Binding of glucose-6-phosphate
B. Dephosphorylation of multiple residues by phosphoprotein in phosphorylase-1 (PP1)
C. Phosphorylation of specific residues by casein kinase II (CKII)
D. Phosphorylation of specific residues by glycogen synthase kinase-3 (GSK-3)
E. The presence if insulin

A

B. Dephosphorylation of multiple residues by phosphoprotein phosphorylase-1 (PP1).

35
Q

Which of the following is not true of the reaction catalyzed by the Pyruvate dehydrogenase complex?
A. Biotin participates in the decarboxylation
B. Both NAD+ and a flavin nucleotide act as electron carriers.
C. The reaction occurs in the mitochondrial matrix.
D. The substrate is held by the lipoyl-lay sine swinging arm.
E. Two different cofactors containing -SH groups participate.

A

A. Biotin participates in the decarboxylation

36
Q
Which of the below is not required for the oxidative decarboxylation of pyruvate to form acetyl-CoA?
A. ATP
B. CoA-SH
C. FAD
D. Lipoid acid
E. NAD+
A

A. ATP

37
Q

Which of the following statements about the oxidative decarboxylation of pyruvate in aerobic conditions in animal cells is correct?
A. One of the products of the reactions of the pyruvate dehydrogenase complex is a thioester of acetate.
B. The methyl group is eliminated as CO2
C. The process occurs in the cytosolic compartment of the cell
D. The pyruvate dehydrogenase complex uses all of the following as cofactors: NAD+, lipoid acid, pyridoxyl phosphate (PLP) and FAD.
E. The reaction is so important to energy production that pyruvate dehydrogenase operates at full speed under all conditions.

A

A. One of the products of the reactions of the pyruvate dehydrogenase complex is a thioester of acetate

38
Q

Glucose labeled with 14C in C3 and C4 is completely converted to acetyl-CoA via glycolysis and the pyruvate dehydrogenase complex. What percentage of the acetyl-CoA molecules formed will be labeled with 14C and in which position of the acetyl moiety will the 14C label be found?
A. 100% Of the acetyl-CoA will be labeled at C-1 (carboxyl)
B. 100% of the acetyl-CoA will be labeled at C2
C. 50% of the acetyl-CoA will be labeled, all at C2 (methyl)
D. No label will be found in the acetyl-CoA molecules
E. Not enough information is given to answer this question.

A

D. No label will be found in the acetyl-CoA molecules.

39
Q

Acetyl-CoA labeled with 14C in both of its acetate carbon atoms is incubated with unlabeled oxaloacetate and a crude tissue preparation capable of carrying out the reactions if the citric acid cycle. After one turn of the cycle, oxaloacetate would have 14C in:
A. All four carbon atoms
B. No pattern that is predictable from the information provided
C. None of its carbon atoms
D. The keto carbon and one of the carboxyl carbons.
E. The two carboxyl carbons

A

A. All four carbon atoms

40
Q

The two moles of CO2 produced in the first turn of the citric acid cycle have their origin in:
A. Carboxyl and methylene carbons of oxaloacetate
B. Carboxyl group of acetate and a carboxyl group of oxaloacetate
C. Carboxyl group of acetate and the keto group of oxaloacetate
D. Two carbon atoms of acetate
E. Two carboxyl groups derived from oxaloacetate

A

E. The two carboxyl groups derived from oxaloacetate

41
Q
The reaction of the citric acid cycle that is most similar to the pyruvate dehydrogenase complex-catalyzed conversion of pyruvate to acetyl-CoA is the conversion of:
A. Citrate to isocitrate
B. Fumarate to malate
C. Malate to oxaloacetate
D. Succinyl-CoA to succinate
E. Alpha-ketoglutarate to succinyl-CoA
A

E. Alpha-ketoglutarate to succinyl-CoA

42
Q

Which of the following enzymatic activities would be deceased by thiamine deficiency?
A. Fumarase
B. Isocitrate dehydrogenase
C. Malate dehydrogenase
D. Succinate dehydrogenase
E. Alpha-ketoglutarate dehydrogenase complex

A

E. Alpha-ketoglutarate dehydrogenase complex

43
Q
The conversion of 1 mol of pyruvate to 3 mol of CO2 via pyruvate dehydrogenase and the citric acid cycle also yields (  ) mol of NADH, (  ) mol of FADH2, and (  ) mol of ATP. 
A. 2;2;2
B. 3;1;1
C. 3;2;0
D. 4;1;1
E. 4;2;1
A

D. 4;1;1

44
Q

Entry of acetyl CoA into the citric acid cycle is decreased when:
A. [AMP] is high
B. NADH is rapidly oxidized through the respiratory chain
C. The ratio of [ATP]/[ADP] is low
D. The ratio of [ATP]/[ADP] is high
E. The ratio of [NAD+]/[NADH] is high

A

D. The ratio of [ATP]/[ADP] is high

45
Q
Citrate synthase and the NAD+ specific isocitrate dehydrogenase are two key regulatory enzymes of the citric acid cycle. The enzymes are inhibited by:
A. Acetyl-CoA and fructose-6-phosphate 
B. AMP and/or NAD+
C. AMP and/or NADH
D. ATP and/or NAD+
E. ATP and/or NADH
A

E. ATP and/or NADH

46
Q

A new compound isolated from mitochondria is claimed to represent a previously unrecognized carrier in the electron transfer chain. It is given the name coenzyme z. Which line of evidence do you feel is the least conclusive in assigning this compound a position in the electron transfer chain?
A. Alternate oxidation and reduction of the mitochondrion bound coenzyme z can be readily demonstrated.
B. Removal of coenzyme z from the mitochondria results in a decreased rate of oxygen consumption
C. The rate of oxidation and reduction of mitochondrion-bound coenzyme is of the same order of magnitude as the overall rate if electron transfer in mitochondria as measured by oxygen consumption.
D. The reduction potential of z is between that of two compounds known to participate in the electron transport chain
E. When added to a mitochondrial suspension coenzyme z is taken up very rapidly and specifically by the mitochondria

A

E. When added to a mitochondrial suspension coenzyme z is taken up very rapidly and specifically by the mitochondria

47
Q

In the redox reoxidation of QH2 by purified ubiquinone-cytochrome c reductase (complex III) from heart muscle, the overall stoichiometry of the reaction requires 2 mol of cytochrome c per mole of QH2 because:
A. Cytochrome c is a one electron acceptor whereas QH2 is a two electron donor
B. Cytochrome c is two electron acceptor whereas QH2 is a one electron donor
C. Cytochrome c is water soluble and operates between the inner and outer mitochondrial membranes
D. Heart muscle has a high rate of oxidative metabolism and therefore requires twice as much cytochrome c as QH2 for electron transfer to proceed normally
E. Two molecules of cytochrome c must first combine physically before they are catalytically active

A

An cytochrome c is a one electron acceptor whereas QH2 is a two electron donor

48
Q

In normal mitochondria the rate of NADH consumption (oxidation) will:
A. Be increased in active muscle, decreased in inactive muscle
B. Be very low if the ATP synthase is inhibited but increase when an uncoupler is added
C. Decrease if mitochondrial ADP is depleted
D. Decrease when cyanide is used to prevent electron transfer through the cytochrome a + a3 complex
E. All of the above are true.

A

E. All of the above are true

49
Q

Which of the following statements about the chemiosmotic theory is correct?
A. Electron transfer in the mitochondria is accompanied by an asymmetric release of protons on one side of the inner mitochondrial membrane
B. It predicts that oxidative phosphorylation can occur even in the absence of an intact inner mitochondrial membrane
C. The effect of uncoupling reagents is a consequence of their ability to carry electrons through membranes
D. The membrane ATP synthase has no significant role in the chemiosmotic theory.
E. All of the above are correct

A

A. Electron transfer in the mitochondria is accompanied by an asymmetric release of protons on one side if the inner mitochondrial membrane

50
Q

2,4-dinitrophenol and oligomycin inhibit mitochondrial oxidative phosphorylation. 2,4-dinitrophenol is an uncoupling agent; oligomycin blocks the ATP synthesis reaction itself. Therefore, 2,4-dinitrophenol will:
A. Allow electron transfer in the presence of oligomycin
B. Allow oxidative phosphorylation in the presence of oligomycin
C. Block electron transfer in the presence of oligomycin
D. Diminish O2 consumption in the presence of oligomycin
E. Do none of the above

A

A. Allow electron transfer in the presence of oligomycin

51
Q

During oxidative phosphorylation the proton motive force that is generated by electron transport is used to:
A. Create a pore in the inner mitochondrial membrane
B. Generate the substrates (ADP and Pi) for ATP synthase
C. Induce a conformational change in the ATP synthase
D. Oxidize NADH to NAD+
E. Reduce O2 to H2O

A

C. Induce a conformational change in the ATP synthase

52
Q
The oxidation of a particular hydroxy substrate to a keto product by mitochondria has a P/Oratio of less than 2. The initial oxidation step is very likely directly coupled to the:
A oxidation if a flavoprotein
B. Oxidation of a pyridine nucleotide
C. Reduction of a flavoprotein
D. Reduction of a pyridine nucleotide
E. Reduction of cytochrome a3
A

C. Reduction of a flavoprotein

53
Q
The relative concentrations of ATP and ADP control the cellular rates of:
A. Glycolysis
B. Oxidative phosphorylation
C. Pyruvate oxidation
D. The citric acid cycle
E. All of the above
A

E. All of the above

54
Q

Mammals produce heat by using the endogenous uncoupling agent:
A. The small molecule 2,4-dinitrophenol synthesized by the cell
B. The protein thermogenin
C. The protein thioredoxin
D. The protein cytochrome c
E. A modified form of the FoF1 ATPase

A

B. The protein thermogenin

55
Q
The initiation of glycogen synthesis occurs by transfer of glucose from UDP-glucose to the:
A. 4-position of free glucose
B. 2-position of fructose
C. Non-reducing end of maltose
D. Serine-OH group of glycogen synthase
E. Tyrosine-OH group of glycogenin
A

E. Tyrosine-OH group of glycogenin

56
Q
Complex I contains all of these components EXCEPT:
A. [FMN]
B. Fe-S
C. NADH
D. NAD+
E. Cyt c
A

E. Cyt c

57
Q

All of the following are properties of ATP synthase EXCEPT:
A. The F1 subunit is attached to the integral membrane protein F0
B. The F0 subunit is hydrophilic
C. Transmembrane channel for protons
D. Beta-subunits have the catalytic site for ATP synthesis
E. The ring of c subunits from a rotor with respect to the alpha subunits

A

B. The F0 subunit is hydrophilic