Final Review Flashcards
For the following reaction,
E. May occur at some concentrations of substrate and product
Which of the following is an electron donor used for reductive biosynthesis? A) NADH B)NADPH C) FADH2 D) CoA E) ATP
B. NADPH
All of the following contribute to the large, negative, free-energy change upon hydrolysis of “high-energy” compounds except:
A) electrostatic repulsion in the reactant.
B) low activation energy of forward reaction.
C) stabilization of products by extra resonance forms.
D) stabilization of products by ionization.
E) stabilization of products by solvation
B. low activation energy of forward reaction.
Biological oxidation-reduction reactions never involve:
A) transfer of e- from one molecule to another.
B) formation of free e-.
C) transfer of H+ (or H3O+) from one molecule to another.
D) formation of free H+ (or H3O+).
E) none of the above.
B. formation of free e-.
What is the purpose of phosphorylating glucose in cytosol?
A) To trap glucose in the cell
B) To destabilize glucose and facilitate the next series of metabolic steps
C) To convert it to a more soluble form
D) All of the above
E) A and B only
E. A and B only
Glycolysis is the name given to a metabolic pathway occurring in many different cell types. It consists of 11 enzymatic steps that convert glucose to lactic acid. Glycolysis is an example of:
A) aerobic metabolism.
B) anabolic metabolism.
C) a net reductive process.
D) fermentation.
E) oxidative phosphorylation.
D. Fermentation
What is the function of a thioester intermediate such as the one formed from GAP?
A) It speeds up the actual reaction so that more product can be made.
B) The thioester shifts the equilibrium of the first stage of the reaction.
C) The thioester allows the two-step reaction to be coupled so the second reaction, the energetically unfavorable phosphorylation, can proceed.
D) The thioester intermediate induces a conformational change that alters the enzyme specificity.
C. The thioester allows the two-step reaction to be coupled so the second reaction, the energetically unfavorable phosphorylation, can proceed.
What is the additional metabolite that is required for the conversion of 3- phosphoglycerate to 2-phosphoglycerate?
A) 1-phosphoglycerate D) 2,3-bisphosphoglycerate
B) Diacylglycerol E) 1,3-bisphosphoglycerate
C) NADH
D) 2,3-bisphosphoglycerate
E) 1,3-bisphosphoglycerate
D. 2,3-bisphosphoglycerate
Fructose can enter glycolysis at two distinct points, depending on the tissue. How is fructose metabolized in adipose tissue?
A) Fructose is cleaved to two molecules of GAP.
B) Fructose is converted to fructose-1-phosphate.
C) Fructose is converted to fructose-6-phosphate.
D) Fructose is cleaved to GAP and DHAP.
E) Fructose is converted to glucose, which enters the pathway.
C. Fructose is converted to fructose-6-phosphate
During strenuous exercise, the NADH formed in the glyceraldehyde 3- phosphate dehydrogenase reaction in skeletal muscle must be reoxidized to NAD+ if glycolysis is to continue. The most important reaction involved in the reoxidation of NADH is:
A) dihydroxyacetone phosphate → glycerol 3-phosphate
B) glucose 6-phosphate → fructose 6-phosphate
C) isocitrate →α-ketoglutarate
D) oxaloacetate → malate
E) pyruvate → lactate
E. Pyruvate –> lactate
In an anaerobic muscle preparation, lactate formed from glucose labeled in
C-3 and C-4 would be labeled in:
A) all three carbon atoms.
B) only the carbon atom carrying the OH.
C) only the carboxyl carbon atom.
D) only the methyl carbon atom.
E) the methyl and carboxyl carbon atoms.
C. Only the carboxyl carbon atom
The first reaction in glycolysis that results in the formation of an energy-rich compound (i.e., a compound whose hydrolysis has a highly negative ΔG’°) is catalyzed by:
A) glyceraldehyde 3-phosphate dehydrogenase.
B) hexokinase.
C) phosphofructokinase-1.
D) phosphoglycerate kinase.
E) triose phosphate isomerase.
A. Glyceraldehyde-3-phosphate dehydrogenase
Gluconeogenesis must use “bypass reactions” to circumvent three reactions in the glycolytic pathway that are highly exergonic and essentially irreversible. Reactions carried out by which three of the enzymes listed must be bypassed in the gluconeogenic pathway?
1) Hexokinase
2) Phosphoglycerate kinase
3) Phosphofructokinase-1
4) Pyruvate kinase
5) Triosephosphate isomerase
A) 1,2,3
B) 1,2,4
C) 1,4,5
D) 1,3,4
E) 2,3,4
D. 1,3,4
Which of the following statements about gluconeogenesis in animal cells is true?
A) A rise in the cellular level of fructose-2,6-bisphosphate stimulates the rate of gluconeogenesis.
B) An animal fed a large excess of fat in the diet will convert any fat not needed for energy production into glycogen to be stored for later use.
C) The conversion of fructose 1,6-bisphosphate to fructose 6-phosphate is not catalyzed by phosphofructokinase-1, the enzyme involved in glycolysis.
D) The conversion of glucose 6-phosphate to glucose is catalyzed by hexokinase, the same enzyme involved in glycolysis.
E) The conversion of phosphoenol pyruvate to 2-phosphoglycerate occurs in two steps, including a carboxylation.
C. The conversion of fructose 1,6-bis phosphate to fructose 6-phosphate is not catalyzed by phosphofructokinase-1, the enzyme involved in glycolysis
1)What family of enzymes is responsible for the first reaction of β-oxidation of fatty acids? A)Tranferases B)Dehydrogenases C)Translocases D)Thiolases E)Phosphatases
B. Dehydrogenases
2)Which of the following choices is correct regarding the hormonal regulation of fatty acid synthesis?
A)The binding of glucagon to its membrane receptors leads to the activation of a protein kinase which phosphorylates acetyl-CoA carboxylase, resulting in inhibition of fatty acid biosynthesis.
B)The binding of glucagon to its membrane receptors leads to the activation of a phosphatase which dephosphorylates acetyl-CoA carboxylase, resulting in inhibition of fatty acid biosynthesis.
C)The binding of glucagon to its membrane receptors leads to the activation of a protein kinase which phosphorylates acetyl-CoA carboxylase, resulting in activation of fatty acid biosynthesis.
D)The binding of insulin to its membrane receptors, leads to the activation of protein kinases, which leads to phosphorylation of acetyl-CoA carboxylase, causing the activation of fatty acid biosynthesis.
E)The binding of insulin to its membrane receptors, leads to the activation of phosphodiesterases, which leads to the dephosphorylation of acetyl-CoA carboxylase, causing the inhibition of fatty acid biosynthesis.
A. The binding of glucagon to its membrane receptors leads to the activation of a protein kinase that phosphorylates acetyl-CoA carboxylase, resulting in inhibition of fatty acid biosynthesis.
3. The glycerol produced from the hydrolysis of triacylglycerides enters glycolysis as: A)glucose. B)glucose-6-phosphate. C)dihydroxyacetone phosphate. D)pyruvate. E)glyceryl CoA.
C. Dihydroxyacetone phosphate
4. Saturated fatty acids are degraded by the stepwise reactions of β oxidation, producing acetyl-CoA. Under aerobic conditions, how many ATP molecules would be produced as a consequence of removal of each acetyl-CoA? A)2 B)3 C)4 D)5 E)6
C. 4
- Which of the following statements concerning the β oxidation of fatty acids is true?
A)About 1,200 ATP molecules are ultimately produced per 20-carbon fatty acid oxidized.
B)One FADH2 and two NADH are produced for each acetyl-CoA.
C)The free fatty acid must be carboxylated in the β position by a biotin-dependent reaction before the process of β oxidation commences.
D)The free fatty acid must be converted to a thioester before the process of β oxidation commences.
E)Two NADH are produced for each acetyl-CoA.
D. The free fatty acid must be converted to a thioester before the process of B oxidation commences
6. If an aerobic organism (e.g., the bacterium E. coli) were fed each of the following four compounds as a source of energy, the energy yield per mole from these molecules would be in the order: A)alanine > glucose > palmitate (16:0) B)glucose > alanine > palmitate C)glucose > palmitate > alanine D)palmitate > alanine > glucose E)palmitate > glucose > alanine
E)palmitate > glucose > alanine
Which of the following is not true of the reaction producing malonyl-CoA during fatty acid synthesis?
A)It is stimulated by citrate.
B)It requires acyl carrier protein (ACP).
C)It requires CO2 (or bicarbonate).
D)One mole of ATP is converted to ADP + Pi for each malonyl-CoA synthesized.
E)The cofactor is biotin.
B. It requires acyl carrier protein (ACP)
- If malonyl-CoA is synthesized from 14CO2 and unlabeled acetyl-CoA, and the labeled malonate is then used for fatty acid synthesis, the final product (fatty acid) will have radioactive carbon in:
A)every C.
B)every even-numbered C-atom.
C)every odd-numbered C-atom.
D)no part of the molecule.
E)only the omega-carbon atom (farthest carbon from C-1).
D. No part of the molecule
- In comparing fatty acid biosynthesis with β oxidation of fatty acids, which of the following statements is incorrect?
A)A thioester derivative of crotonic acid (trans-2-butenoic acid) is an intermediate in the synthetic path, but not in the degradative path.
B)A thioester derivative of D-β-hydroxybutyrate is an intermediate in the synthetic path, not in the degradative path.
C)Fatty acid biosynthesis uses NADPH exclusively, whereas β oxidation uses NAD+ exclusively.
D)Fatty acid degradation is catalyzed by cytosolic enzymes; fatty acid synthesis by mitochondrial enzymes.
E)The condensation of two moles of acetyl-CoA in the presence of a crude extract is more rapid in bicarbonate buffer than in phosphate buffer at the same pH; the cleavage of acetoacetyl-CoA proceeds equally well in either buffer.
D. Fatty acid degradation is catalyzed by cytotoxic enzymes, fatty acid synthesis by mitochondrial enzymes