Final: Oxidative Phosphorylation Flashcards
Brown fat has lots of _________ to…
Mitochondria to keep baby warm via oxidation.
The inner membrane of the mitochondria is __________ and the outer membrane is selectively _________.
Impermeable
Permeable
What is the inner membrane usually rich in?
Protein, half of which is directly involved in ET and OXPHOS
Where does ETC happen?
Inner membrane
What are ATP synthase complexes?
Complexes of proteins that contain domains that span the inner mitochondrial membrane
T/F: The synthesis of glucose, urea, and heme occur completely in the matrix of the mitochondria.
False, it occurs partially in the matrix
Where would you find Flavin?
Inner mitochondrial membrane
Does the intermembrane space have high or low pH and why?
Low pH because it has a lot of free H+ ions
T/F: As electrons move down ETC (through complexes) their energy decreases.
True
Which complex does the ETC begin on?
Either complex 1 or 2…which determines how much ATP made at end
What are the different complexes considered?
Complex 1-4 are protein complexes
Complex 5 is ATP sythase complex
What do statins block?
An intermediate in the synthesis of CoQ 10
What two complexes can Cytochrome C bound between?
Complexes III & IV
What complexes does CoQ 10 bounce between?
Complexes I & III
Where is Fe sulfate located?
Complex I
How many protons do you get at the end of complex IV if you start at complex I VS. complex II?
10 if you start at complex I
6 if you start at complex II
Does the passing of electrons cause a release or gain in energy?
Release because you are going from a - value to a + value which mathematically shows the thermodynamics
What causes the uncoupling of the ETC with the ATP synthase?
The UCP-1 channel allows H+ to flow back into the matrix and not creating ATP, therefore creating heat (explosions of ∆G)
What percentage of H+ continues to go through ATP synthase mechanism when UCP-1 is expressed?
~20%
What is the F1 component of the ATP synthase complex?
Makes ATP
Composed of alpha, beta, gamma, delta, epsilon
What is the F0 component of the ATP synthase complex?
Translocates proteins
Composed of A, B2, & C-ring
What are the rotating components of the router?
C-ring, gamma, & epsilon
What are the T, L, & O components of the ATP synthase?
Tight- synthesizes ATP
Loose- loosely binds ADP
Open- releases newly formed ATP
Which direction does the router spin?
Always counterclockwise
