Final: Oxidative Phosphorylation

Question 0

Brown fat has lots of _________ to…

Answer 0

Mitochondria to keep baby warm via oxidation.

Question 1

The inner membrane of the mitochondria is __________ and the outer membrane is selectively _________.

Answer 1

Impermeable

Permeable

Question 2

What is the inner membrane usually rich in?

Answer 2

Protein, half of which is directly involved in ET and OXPHOS

Question 3

Where does ETC happen?

Answer 3

Inner membrane

Question 4

What are ATP synthase complexes?

Answer 4

Complexes of proteins that contain domains that span the inner mitochondrial membrane

Question 5

T/F: The synthesis of glucose, urea, and heme occur completely in the matrix of the mitochondria.

Answer 5

False, it occurs partially in the matrix

Question 6

Where would you find Flavin?

Answer 6

Inner mitochondrial membrane

Question 7

Does the intermembrane space have high or low pH and why?

Answer 7

Low pH because it has a lot of free H+ ions

Question 8

T/F: As electrons move down ETC (through complexes) their energy decreases.

Answer 8

True

Question 9

Which complex does the ETC begin on?

Answer 9

Either complex 1 or 2…which determines how much ATP made at end

Question 10

What are the different complexes considered?

Answer 10

Complex 1-4 are protein complexes

Complex 5 is ATP sythase complex

Question 11

What do statins block?

Answer 11

An intermediate in the synthesis of CoQ 10

Question 12

What two complexes can Cytochrome C bound between?

Answer 12

Complexes III & IV

Question 13

What complexes does CoQ 10 bounce between?

Answer 13

Complexes I & III

Question 14

Where is Fe sulfate located?

Answer 14

Complex I

Question 15

How many protons do you get at the end of complex IV if you start at complex I VS. complex II?

Answer 15

10 if you start at complex I

6 if you start at complex II

Question 16

Does the passing of electrons cause a release or gain in energy?

Answer 16

Release because you are going from a - value to a + value which mathematically shows the thermodynamics

Question 17

What causes the uncoupling of the ETC with the ATP synthase?

Answer 17

The UCP-1 channel allows H+ to flow back into the matrix and not creating ATP, therefore creating heat (explosions of ∆G)

Question 18

What percentage of H+ continues to go through ATP synthase mechanism when UCP-1 is expressed?

Answer 18

~20%

Question 19

What is the F1 component of the ATP synthase complex?

Answer 19

Makes ATP

Composed of alpha, beta, gamma, delta, epsilon

Question 20

What is the F0 component of the ATP synthase complex?

Answer 20

Translocates proteins

Composed of A, B2, & C-ring

Question 21

What are the rotating components of the router?

Answer 21

C-ring, gamma, & epsilon

Question 22

What are the T, L, & O components of the ATP synthase?

Answer 22

Tight- synthesizes ATP
Loose- loosely binds ADP
Open- releases newly formed ATP

Question 23

Which direction does the router spin?

Answer 23

Always counterclockwise