Final Lecture Exam (new) Flashcards
light rxns:
pigment molecules (like chlorophylls) are critical to the light reactions bc they _____
capture light energy
light rxns:
chlorophylls are contained w/in structures called ___
photosystems
light rxns:
photosystems are located in ___
thylakoid membranes
light rxns:
photon energy is captured by ___ contained in ___
chlorophylls contained in photosystems
light rxns:
photon capture (mechanism)
antenna chlorophylls (AC) capture photon energy
photon energy radiated from AC to AC
energy captured by the reaction center chlorophyll (RCC)
energy is absorbed by electrons in the RCC
energized electrons are:
1) ejected from RCC
2) captured by an electron carrier
3) enter into an electron transport chain
ejected electrons are replaced
light rxns:
where are the photosystems located?
thylakoid membrane
light rxns:
PS2 gets replacement electrons from ___
H2O
light rxns:
PS1 gets its electrons from ___
PS2
light rxns:
in both PS1 and PS2, antenna chlorophyll ___
capture photon/light energy
light rxns:
in both PS1 and PS2, photon energy is used to ___ w/in ___
energize electrons w/in reaction center chlorophylls (RCC)
light rxns:
in PS2, energized electrons enter the ___ and are transported from ___
ETC and are transported from PS2 to PS1
light rxns:
in PS2, energy from the electrons in ETC is used to ___
produce ATP
light rxns:
in PS2, ___ is used to produce ATP
energy from the electrons in ETC
light rxns:
in PS2, replacement electrons come from ___
H2O
light rxns:
in PS2, ___ enter the ETC and are transported from PS2 to PS1
energized electrons
light rxns:
in PS2, ___ come from H2O
replacement electrons
light rxns:
in PS1, replacement electrons come from ___
PS2
light rxns:
in PS1, ___ come from PS2
replacement electrons
light rxns:
in PS1, de-energized electrons from PS2 are ___ w/ ___
re-energized w/ photon energy
light rxns:
in PS1, ___ from PS2 are re-energized w/ photon energy
de-energized electrons
light rxns:
in PS1, energized electrons are transferred to ___, thereby ___ to ___
NADP+
thereby reducing it to
NADPH
light rxns:
in PS1, ___ are transferred to NADP+, thereby reducing it to NADPH
energized electrons
light rxns:
in PS2, energized electrons get ejected and enter into the ___
ETC
light rxns:
in PS2, ___ ___ get ejected and enter into the ETC
energized electrons
light rxns:
the ___ carries electrons from PS2 to PS1
ETC
light rxns:
when electrons reach PS1, they have ___ ___ ___
lost their energy
light rxns:
when electrons reach ___, they have lost their energy
PS1
light rxns:
where does lost electron energy in PS1 go?
energy stored in electrons at PS1 is used to power a H+ pump
light rxns:
what does the H+ pump do?
what are the protons doing thru the proton pump?
creates a proton gradient across the membrane
protons are flowing from stroma into inner thylakoid space via the proton pump
light rxns:
to diffuse back across the ___, H+s need a ___
membrane
channel
light rxns:
what is the channel that allows H+s to diffuse back across the membrane?
what type of molecule is it?
ATP Synthase
enzyme/transport protein
light rxns:
ATP Synthase uses the ___ to make ATP
energy of H+ flow
light rxns:
ATP Synthase uses the energy of H+ flow to ___ ___
make ATP
light rxns:
how light reactions make ATP (mechanism)
excited electrons pass from PS2 to PS1 thru the ETC
ETC powers proton pump which builds up a H+ gradient on the inner thylakoid membrane
protons flow back out into stroma thru ATP Synthase
ATP Synthase spins as protons flow thru –> generates ATP
light rxns:
how light reactions make NADPH (mechanism)
photon energy from light is used to re-energize the electrons in PS1
re-energized electrons are used to reduce NADP+ to NADPH
where do dark reactions occur?
stroma of chloroplast
dark reactions require…
NADPH (produced by light rxns)
ATP (produced by light rxns)
CO2
dark reactions occur in 3 steps
- carbon fixation
- reduction of PGA
- regeneration of RuBP
carbon fixation (general)
___ step of the dark rxns
converts an ___ form of carbon (specify) into an ___ form (specify)
1st step of dark rxns
converts an inorganic form of carbon (CO2) into an organic form (PGA)
reduction of PGA (general)
___ step of the dark rxns
electrons are transferred to ___ from ___
2nd step of dark rxns
electrons are transferred to PGA from NADPH
regeneration of RuBP (general)
___ step in dark rxns
3rd step of dark rxns
RuBP is a molecule required for the carbon fixation step
carbon fixation (detailed mechanism)
begins w/ RuBP and CO2
RuBP & CO2 form a covalent bond:
1 RuBP + 1 CO2 –> 6-C molecule
this rxn is catalyzed by enzyme Rubicso
Next:
6-C molecule spontaneously breaks down into two, 3-C molecules (PGA):
one, 6-C molecule –> two, 3-C PGA molecules
RuBP (what is it)
5-C sugar known as the “carbon acceptor”
rubisco (definition)
enzyme that catalyzes the addition of CO2 to RuBP
reduction of PGA uses
NADPH as electron source
ATP as energy source
reduction of PGA (overview)
PGA is reduced and converted: PGA –> G3P
G3P has 2 functions
used to make glucose
used to regenerate RuBP for carbon fixation
carbon fixation ends in the production of ___ PGA molecules
2
carbon fixation ends in the production of 2 ___
PGA molecules
___ ___ ends in the production of 2 PGA molecules
carbon fixation
summary: dark rxns
carbon fixation
5-C (carbon) RuBP + CO2 –(Rubisco enzyme)–> 6-C intermediate that breaks down into 2, 3-C molecules (PGA)
summary: dark rxns
reduction of PGA
3-C molecules (PGA)
Using energy from ATP and electrons from NADPH:
2, 3-C PGA molecules –> G3P molecules
summary: dark rxns
RuBP regeneration
using G3P molecules:
some G3P used to make more RuBP
some G3P used to make glucose
*glucose is not only molecule made by dark rxns
dark rxns and metabolism:
the DRs feed into many different ___ pathways
synthesis
dark rxns and metabolism:
DRs feed into many different synthesis pathways:
other sugars
amino acids
lipids
nucleic acids
glucose as fuel:
glucose contains lots of ___
energy
glucose as fuel:
glucose ∆G = ___
ATP Hydrolysis = ___
- 686 kcal/mol
- 7.3 kcal/mol
how do organisms extract the energy from glucose?
thru the oxidation of glucose
oxidation of glucose has 2 phases
glycolysis and cellular respiration
oxidation of glucose (2 phases):
phase 1 is ___
glycolysis
oxidation of glucose (2 phases):
phase 1, glycolysis:
occurs in the ___
glucose is converted into ___
cytoplasm of cells
pyruvate
oxidation of glucose (2 phases):
phase 2 is ___
cellular respiration
oxidation of glucose (2 phases):
phase 2, cellular respiration:
occurs in ___
mitochondria of cells
oxidation of glucose (2 phases):
phase 2, cellular respiration:
CR has 3 stages:
oxidation of pyruvate
citric acid cycle (TCA, Krebs cycle)
electron transport chain
oxidation of glucose (2 phases):
these stepwise processes allow for a controlled, regulated release of energy from ___
glucose
oxidation of glucose (2 phases):
these stepwise processes allow for a controlled, regulated release of ___ from glucose
energy
oxidation of glucose (2 phases):
these stepwise processes allow for a ___, ___ release of energy from glucose
controlled, regulated
oxidation of glucose (2 phases):
these ___ ___ allow for a controlled, regulated release of energy from glucose
stepwise processes
ATP production:
during Glycolysis and cellular respiration, ATP can be generated in how many ways?
2
ATP production:
what are the 2 ways ATP can be produced during glycolysis and cellular respiration?
chemiosmosis
substrate-level phosphorylation
ATP production:
chemiosmosis (definition)
flow of protons thru ATP synthase
ATP production:
substrate-level phosphorylation (steps)
an enzyme:
1) takes a phosphate from 1 molecule
2) adds a phosphate to ADP:
ADP –> ATP
glycolysis occurs in the ___
cytoplasm
___ occurs in the cytoplasm
glycolysis
in glycolysis, glucose is converted to ___
pyruvate
in ___, glucose is converted to pyruvate
glycolysis
in glycolysis, ___ is converted to pyruvate
glucose
glycolysis is a ___-step process
10
inputs of glycolysis
1 glucose (6-C)
2 NAD+
2 ADP
outputs of glycolysis
2 NADH (reduction of NAD+) 2 ATP (substrate-level phos.) 2 pyruvate (3C)