Final: Lecture 28

Question 0

T/F The inner mito membrane is extremely permeable.

Answer 0

• False, extremely impermeable

* Outer membrane selectively permeable, big things can get through

Question 1

What tissues are the richest in mitochondria?

Answer 1

•Brain and Muscles

Question 2

Over 50% of the mito proteins are made in the?

Answer 2

•Nucleus

Question 3

How is mitochondria like sitting by a fireplace?

Answer 3

• Has a role in warming the body, especially in new borns
• Brown fat high in mito, keep the baby warm
• Firewood similar to burning fuel in mito, in this case glucose derivatives or fatty acids

Question 4

Reduce NADH and FADH2 bring…

Answer 4

• e-, Vit B and other energy vitamins
• e- handed from one complex to another, within complex 1 there are 5-10 handoffs going down hill, decreasing ∆G more after each handoff

Question 5

The pH in the intermembrane space is?

Answer 5

•Low

Question 6

________ is the ultimate e- acceptor.

Answer 6

•Oxygen

Question 7

Electrons flow in Complex I from NADH through ______ and a series of iron sulfur clusters to ________.

Answer 7

• FMN (flavin mononucleotide)
• Ubiqinone (Q)
• FAD (flavin adenine dinucleotide)
• Flavin proteins are a component of ETC

Question 8

Complexes are somewhat fixed, Co Q can go between Complexes __ and __, and Cyto C can go between __ and __.

Answer 8

• I and III
• III and IV
• Cytochrome are a heme containing protein, proforin ring structur

Question 9

∆Gº’ = -nF∆E’

Answer 9

• n = number of e-
• F is faraday’s constant
• ∆E’ is reduction potential

Question 10

How does brown fat convert e- flow into warmth?

Answer 10

•Protons just pump e- in and back out, don’t yield ATP

Question 11

What is the role of ATPsynthase?

Answer 11

• When the proton gradient goes through the system, it turns the wheel to make ATP
• Made up of F1 and F0 subunits

Question 12

What is the role of the F1 subunit?

Answer 12

• Makes the ATP, ATPase portion

* Subunits alpha, beta, gamma, delta, epsilon

Question 13

What is the role of F0 subunit?

Answer 13

• Protein translocator

* Made up of the b2, a, and c ring subunits

Question 14

The rotor spins because of _______ coming through the membrane.

Answer 14

•Protons

Question 15

T/F the spinning rotor spins in a counterclockwise direction.

Answer 15

•True

Question 16

What are the subunits of the spinning rotor?

Answer 16

• C ring, and gamma/epislon (everything else stationary component)
• Mechanical spinning transduces energy necessary to make ATP
• Whole complex takes energy from downhill movement of protons and makes ATP

Question 17

What is the driving force of this process to make ATP?

Answer 17

•ADP*

Question 18

What are the 3 conformations of the ring?

Answer 18

• T = Tight, synthesis ATP
• L = Loose, binds ATP
• O = Open, releases newly formed ATP