Final Exam Chapters 15, 16, 17, 18 Flashcards
Cloning
Began with study of bacteria and bacteriophage
Bacteriophage
Virus that attacks bacteria
Recombinant DNA
1980’s, first constructed by Paul Berg, molecule that has DNA from both sources
Restriction Enzymes
Molecular scissors for DNA, use same enzyme on DNA vector and plasmid
Vector
Inserted into Plasmid
Hind III
Restriction Enzyme, produces sticky ends so vector can join plasmid tightly
Plasmid
Circular DNA from bacteria, carries foreign DNA into a host bacterial cell and replicates
Recombinant DNA
DNA that has foreign DNA spliced in it. If replicated, foreign DNA is cloned
Measurement of DNA
Weight in KB = Kilobases 1KB= 1000 kilobases
Genome
Total DNA in a cell
DNA Probing
Homologous to part of sequence of interest, segment of single stranded DNA that can hyberdize
Hyberdize
Attach by base pairing to complementary base sequences in target DNA
Polymerase Chain Reaction
developed by Kary Mullis in 1985, Replicating DNA outside your body using machine
Studying Genes
- Silence genes 1 at a time
2. Target genes “gene targetting”, produce knockout organism
Orthologous genes
Homologous genes found in different species inherited from same ancestor.
Transgenic Organism
Put foreign genes into plant or animals
ex. Genetically Modified Food
Chromosomes 1 and 21
1 should be the largest, 21 should be the smallest
Human Genome Project
Majority of human DNA has noncoding sequences, don’t know their function
Restriction Fragment Length Polymorphism
Used during paternity test, determine how close individuals are genetically
Comparative Genomics
Compare genomes of different species
Metagenomics
Used to analyze communities of microorganisms instead of individual organisms
Polyploidy
Abnormalities in Chromosome. Multiple sets of chromosomes. Common in plants, rare in humans. Due to failure of chromosomes to separate during meiosis
Aneuploidies
either 1 extra or 1 less chromosome, failure of chromosomes to separate at anaphase.
Trisomy and Monomy are aneuploidies.
Sex Aneuploidies
Less severe than autosomal.
XO female can survive
OY male will not survive
XYY very aggressive male
Klinefelter Syndrome
XXY, male more feminine, produce little to no sperm, have breasts
Turners Syndrome
XO, underdeveloped female
XYY
Very developed male, lots of acne, criminal tendencies.
Inversion
Orientation of chromosome is reversed