FINAL EXAM Flashcards
define microbiology
study of microscopic life
Hooke
cell theory
van leuwenhoek
discovered microscope and microorganisms
pasteur
- final disproof of spontaneous generation
- pasteurization: mild heat
- fermentation: sugar converted to alcohol
redi
- 3 jars of meat
- biogenesis
koch
- bacteria cause disease (germ theory)
- koch’s postulates
define spontaneous generation
life can spontaneously generate
- living organisms can come come from non living matter
- redi proved biogenesis
define cell theory
- cells are the smallest form of life and make up everything
- hooke
define germ theory
- microorganisms cause disease
- different organisms cause different diseases: koch
define bacteriology
study of bacteria
define mycology
study of fungi
define virology
study of viruses
define phycology
study of algae
What are the five kingdoms of life
- monera: archaea and bacteria
- protista: algae and protozoa
- fungi: yeasts and molds
- animals
- plants
Binomia nomenclature
- define
- rules
- who made it
- rules for naming organisms
- capitalize Genus but not species
- underline and italicize both
- carl von linne
define polymer
large macromolecules made by putting smaller macromolecules together
What are the major macromolecules and elements do they contain
- Carbs, proteins, lipids, nucleic acids
- C, H,O, n
Carbohydrates
- building blocks
- bonds
- enzymes
- function
- simple sugars/ polysaccharides
- glycosidic bonds
- hydrolysis
- nutrients, energy, support, protection
Proteins
- building blocks
- bonds
- enzymes
- function
- amino acids: carboxyl group+amino group+R group
- peptide bonds
- peptidase
- structure and catalysts
3 types of lipids
- triglycerides
- phospholipids
- steroids/waxes
Triglycerides
- building blocks
- bonds
- enzymes
- function
- glycerol and 3 fatty acids
- ester bonds
- lipase
- lots of energy storage
Phospholipids
- building blocks
- bonds
- enzymes
- function
- glycerol+2 fatty acids+phosphate group+ organic group
- ester bonds
- lipase
- cell membrane structure
Steroids and waxes function of each
- animal hormones
- mycolic acid
Nucleic Acids; DNA and RNA
- building blocks
- bonds
- enzymes
- function
- nucleotides: base/pentose/phosphate group
- phosphodiester and hydrogen(DNA)
- nuclease
- genetic info and protein synthesis
DNA
- double stranded
- thymine
- millions of nucleotides
- genetic info
RNA
- single stranded
- uracil
- thousands of nucleotides
- protein synthesis
explain the lock and key model of enzymes
enzymes are specific for a particular substrate
Factors that influence enzymes activity
- PH: optimal at 7
- temp: higher temp higher activity
- concentration: high concentration higher activity
Prokaryotes: exterior cell structures
- flagella
- pili
- fimbriae
- axial filaments
prokaryotic flagella
long slender thread like appendages composed of protein
- propeller like motility
prokaryotic axial filaments
- protein fibrils wound around organism and attached at two poles
- rapid motility in cork screw motion
prokaryotic fimbriae
- short hairlike fibers composed of protein
- adherence factor
prokaryotic pilli
- elongated and tubular
- bacterial conjugation: transfer DNA using plasmids
Prokaryotes: cell surface layers
- capsule
- cell wall
- plasma membrane
Prokaryotic capsule
- glycocalyx
- exterior coating of cell wall
- capsule or slime layer
- made of carbs and proteins
prokaryotic cell wall
- peptidoglycan
- shape, support, flagella attachment
plasma membrane
- phospholipid bilayer and proteins
- allow material to enter or exit
gram + cell wall
thick layer of peptidoglycan + teichoic acid
gram - cell wall
thin layer of peptidoglycan + outer plasma membrane
prokaryotes: internal cell structure
- cytoplasm
- nucleoid
- ribosomes
- inclusion bodies
- endospores
-plasmids
prokaryotic cytoplasm
gelatinous substance of cell inside plasma membrane
prokaryotic nucleiods
single long continuously arranged threas of DNA tightly coiled around proteins
- bacterial chromosome/genetic info
prokaryotic ribosomes
- free or bound to cell membrane
- 2 subunits
- protein synthesis
prokaryotic plasmids
- small circular extrachromosomal dna
- transfered by pili
inclusion bodies
- storage vessels or reserves for nutrients
bacterial cell measurement
micrometer= 1/1,000 mm
Unusual prokaryotes
- rickettsia
- chlamydiae
- mycoplasma
- bdellovibrios
- actinomycetes
unusual rickettsia
- obligate intracellular organism
- small
- need arthropod vector
- RASH
unusual chlamydiae
- obligate intracellular
- small
- elementary-reticulate-elementary
- binary fission reproduction
unusual mycoplasma
- no cell wall
- fried egg colony appearance
- smallest organism capable of living outside of host cell
unusual bdellovibrios
- leech/curved
- parasitize other gram - rods
unusual actinomycetes
- actinomyces, nocardia, streptomyces
- filamentous appearance
Eukaryotes: exterior cell structure
- flagella
- cilia
eukaryotic flagella
long projection composed of 9+2 microtubules
- much thicker than prokaryotes and few per cell
- mermaid motility
eukaryotic cilia
- like flagella but short and many per cell
- movement and feeding
Eukaryotes: cell surface layers
- cell wall
- plasma membrane
eukaryotic cell wall
- carbs and no peptidoglycan
- algae, fungi, plants
- shape and protection
Eukaryotes: interior cell structures
- organelles
- cytoskeleton: microfilaments, intermediate, microtubules
eukaryotic cytoskeleton
- cell skeleton
- support, shape, transport
- microfilaments, intermediate, microtubules
Eukaryotic nucleus
- command center
- RNA synthesis and DNA genetic info
Eukaryotic mitochondria
- power plant
- generate energy in the form of ATP
eukaryotic ribosomes
- protein factories
- mede in nucleus
eukaryotic endoplasmic reticulum
- rough: protein synthesis, packaging
- smooth: lipid synthesis
eukaryotic Golgi body
- protein packager
- modifies and targets proteins
eukaryotic lysosomes
- garbage disposal
eukaryotic microorganisms
- algae
- fungi
- protozoa
what eukaryotic organism is photosynthetic
algae
reaction for photosynthesis
6CO2 + 6H20 = C6H12O6 + 602
what photopigment do all algae possess that make some green
chlorophyll but other photopigments present as well
how are protozoa classified
locomotion
do protozoa have members involved in human disease
all classes of protozoa have members involved in human disease
dimorphic fungi
mold in nature and yeast in the body
how do yeast reproduce and what are they
budding and single cells
how do molds reproduce and what are they
spore formation and filaments of cells called hyphae
what is a mass of hyphae called
mycelium
Are viruses RNA or DNA
either RNA or DNA but not both
define nucleocapsid
nucleic acid with viral capsid
how are viruses measured
nanometers
explain icosahedral structure
- polyhedral
- 20 faces throughout
explain helical structure
- nucleic acid and capsomeres helically coiled together
- Hallow rod
how is a viral envelope acquired
as virion buds through cell membrane
purpose of viral spikes
allow viruses to penetrate host cells and cause infection
- can only be on enveloped structures
5 stages of interaction between animal virus and host cell
- attachment
- penetration
- uncoating
- assembly
- release
attachment stage of virus
most important stage for stopping infection
penetration stage of virus
endocytosis or membrane fusion
uncoating phase of virus
replication, transcription, translation
assembly stage of virus
DNA in nucleus , RNA in cytoplasm
release stage of virus
lysis without an envelope and budding with envelope
explain endocytosis
- no envelope
- engulfment of virions
explain membrane fusion
- envelope fuses with host cell
- nucleocapsid move in
explain lytic cycle
- phage attaches, penetrates, and injects itself into dna which is assembled into virions
- cell bursts and virions are released
explain lysogenic cycle
phage attcahes and infects dna in chromosome: prophage
- cell division
DNA cancers
- epstein barr
- hepatitis b
- Herpes virus 8
- HPV
RNA cancers
- retrovitus
- HIV
-AIDS - hepatitis C
TSG
Suppress proliferation
- mutations cause cancer
protooncogenes
stimulate proliferation
- mutations can cause cancer
physical requirements for bacterial growth
- temp: 37
- pH: 6-8, 7-7.2bacteria, 5-6 fungi
- gas: CO2 and O2
- osmotic pressure: isotonic conditions
chemical requirements for bacterial growth
- Carbon
- Nitrogen
- Phosphorus
- Sulfur
-… oxygen for most
how do bacteria reproduce
binary fission
process of binary fission
- cell elongates/dna replication
- invagination at mid point
- cross wall formation
- cells separate
bacterial growth curve phases
- lag
-log - stationary
- death
lag phase of growth
- cells adapting
- little to no cell division
log phase of growth
- rapid cell division
- lots of nutrients
-cells most vulnerable
stationary phase of growth
- nutrients decrease
- toxins present
- cell death=cell growth
death phase of growth
- nutrients depleted
- toxins increase
- cell death > cell growth
methods to measure bacterial growth
- turbidity
- direct microscopic count
- standard plate count