(Final) Cultivation of viruses Flashcards
T/F: It is easier to cultivate bacteria than cultivating viruses
TRUE
T/F: Viruses have the genetic capability to multiply by division
FALSE
Viruses need a living host cell to replicate and generate next progeny viruses
What are some ways used to grow/cultivate viruses?
Cell tissue culture
inoculation in embryonated egg
laboratory animals
What are monolayer cultures?
Cell cultures that use cells grown in-vitro and are dispersed as a single layer on a solid surface (flat)
Compared to a cell suspension in a test tube
What is the best culture system for isolation and propagation of viruses?
Primary cell culture
***used in vaccine production
What is the term for the transfer of cells from one culture vessel to another culture vessel?
why is this done?
Subculture or passage
*this is periodically required to provide fresh nutrients and growing space for continuously growing cell lines
What are two cell lines used to grow viruses?
Finite diploid cell lines: cell lines which have a LIMITED lifespan and go through a LIMITED number of cell generations
Continuous cell lines: acquire the ability to divide indefinitely
Where are finite cell lines derived from?
Mainly from embryos, or from secondary cell cultures
These are more homogenous than primary cell lines - more cells of the same type
*easier to use
T/F: Finite cell lines exhibit contact inhibition
TRUE
What cell lines are used for vaccine production?
Finite cell lines
What kind of cells are used as continuous cell lines?
Typically cancer cells/oncogenic cells
**These are the MOST homogenous
Do continuous cell lines exhibit contact inhibition?
NO
***this is why they are not used for vaccine production (they are genetically abnormal)
What is used to provide cell lines with nutrients for growth?
Culture medium
Ex: Eagles basal medium
What is the most widely used animal serum supplement for cell growth in culture? What is its function?
FBS - fetal bovine serum
Required for growth and maintenance of cells, helps in cell adhesion, regulates cell membrane permeability, and provides nutrients
What is the purpose of Phenol Red pH indicator?
Indicates change in pH (a decrease) by changing the color of the medium from red to orange
Helps you monitor cell cultures. The pH drop is due to cell excretions over time or fungal contamination
