FINAL Flashcards
Cardiac Output using Ohm’s law
(MAP-CVP/SVR) x 80
Partial pressure of substance (outside body)
Patm x % of molecules
Partial pressure of inspired gas (PiO2)
% x (Patm-PH20) partial pressure of H20 is 47
Pressure of oxygen in alveoli (PAO2)
[FiO2 x (Patm - PH20)] - PaCO2/0.8
How do you calculate A-a gradient and what is the normal?
PAO2- PaO2 (5-15 mmHg)
Law of Laplace (sphere/alveoli)
P= 2T/R
Compliance
Delta V/Delta P or (TV/PIP-PEEP)
Boyle’s Law/Ideal gas law
PV=nRT (as pressure drops, volume increases)
Alveolar Ventilation
(TV-DV) x RR
How do you calculate dead space (DV)?
2 ml/kg of IBW or PaCO2-PeCO2/PaCO2 (take % and apply to TV)
Total O2 Content
(1.39 x Hgb x %sat) + 0.003 PO2
Dissolved CO2
PaCO2 x 0.067
IBW
Women 105 + 5 lbs for every in over 5’ Men 106 + 6 for every in over 5’
Calculating Stroke Volume
EDV-ESV
Calculating EF
(EDV-ESV)/EDV x 100
Calculating MAP
1) SBP-DBP 2) divide by 3 3) add that to diastolic
Pulse Pressure
SBP-DBP
Coronary Perfusion Pressure
DBP- LVEDP
What % of CO does the liver get?
25%
What are the functional units of the liver?
hepatic lobule or acinus
Normal hepatic blood flow
1500 mL/min
How much blood flow comes from the hepatic artery?
25-30%
How much blood flow comes from the portal vein?
70-75%
O2 sat of blood from portal vein
60-85%
How much oxygen does the hepatic artery supply?
45-50% of requirements
How much oxygen does the portal vein supply?
50-55% of requirements
Mean BP of hepatic artery
40-70
Mean BP of portal vein
5-10
Why is the dual supply to the liver important?
makes it resistant to hypoxia
Hepatic arterial flow is dependent on…
autoregulation
Portal vein flow is dependent on…
blood flow to GI tract and spleen
What happens to liver blood flow after meals?
increases
What vessels converge to form the hepatic portal vein?
superior mesenteric, inferior mesenteric, and splenic veins
Receptors in Hepatic artery vs portal vein
artery- alpha and beta vein- only alpha
Kupffer cells
remove bacteria and endotoxin entering the blood stream from the portal circulation- part of reticuloendothelial system
Metabolic functions of the liver
carbs, fats, proteins, drugs
Final products of carb digestion
glucose, fructose, galactose
Hepatic conversion of fructose and galactose into ? makes ? metabolism the final common pathway for most carbs
glucose
Cells use ? to produce ? either aerobically via the citric acid cycle or anaerobically via glycolysis
glucose; ATP
Anion gap
(Na+K)-(Cl+HCO3)
plasma osmolarity
2(Na+K)+0.055(glucose)+0.36(BUN)
Aortic root pressure
1/3(LVP-aortic root P)
Coronary perfusion pressure
DBP-CVP
Cerebral perfusion pressure
MAP-ICP or MAP-CVP
Filtration fraction
GFR/RBF
Glomerular filtration pressure
MAP-(colloidal P+glomerular P)
Glucose is stored in hepatocytes as ?
glycogen
gluconeogenesis
liver produces glucose from lactate, pyruvate and amino acids (maintains normal glucose)
What stimulates and what inhibits gluconeogensis?
stimulates- glucocorticoids, catecholamines, glucagon, thyroid hormones inhibits- insulin
Glycogenolysis
release of glucose back into circulation
What stimulates glycogenolysis?
surgical stress, SNS stimulation
Liver makes plasma proteins except?
immunoglobulins
What coagulation factors does the liver NOT make?
VIII, vWF
What is produced by the liver that is important in regards to succinylcholine?
pseudocholinesterase
The liver combines 2 ammonia with CO2 to produce?
urea- excreted by kidneys
What are excess carbs and proteins converted into?
fatty acids
Fatty acids are oxidized into…
acetyl CoA
Acetyl CoA is used…
in cirtic acid cycle to produce ATP
Where is Acetyl CoA stored?
liver and adipose tissue
Obstructive vs. parenchymal disorders of the liver
obstructive- affect biliary excretion parenchymal- generalized hepatocellular dysfunction
What liver test is considered a true test of function?
serum bilirubin- reflects liver’s ability to take up, proess, and secrete bilirubin
Normal serum bilirubin
less than 1.2-1.5 mg/dL
van den Bergh reaction
indirect positive- unconjugated bilirubin biphasic- both unconjugated/conjugated direct positive- conjugated
What does high unconjugated bilirubin mean?
hemoglobin is being broken down too fast or the liver can’t process it fast enough (neurotoxic –> encephalopathy)
What does high conjugated bilirubin mean?
bilirubin is backing up in the liver
normal albumin
3.5-5.3 g/dL
What does low albumin indicate?
liver disease, nephrotic syndrome- half life is 14 days, so values are normal in acute liver disease
Prothrombin time (PT)- what is it and normal range?
measures how long it takes for clot to form (activity of fibrinogen, prothrombin, and factors V, VII, X)- 10-14 seconds
Normal platelet count
150,000-450,000 mm3
What does a prolonged PT mean?
severe liver disease (unless Vit K deficiency is present)
Transaminases
enzymes released into circulation as result of hepatocellular injury/death (AST and ALT)
AST
associated with cell necrosis (liver, brain, heart tissues)
ALT
LIVER CELLS ONLY- indicate cell inflammation
Blood ammonia is cleared by the liver and converted into?
urea
Normal ammonia levels
47-65 mmol/L
What does an elevation in ammonia indicate?
disruption of urea synthesis, severe hepatocellular damage, can cause encephalopathy
What do inhalation agents do to hepatic blood flow?
DECREASE- halothane is the worst *(isoflurane is the least- causes arterial dilation that can actually increase hepatic BF)
What does regional anesthesia do to hepatic blood flow?
decrease by lowering arterial BP
What does general anesthesia do to hepatic blood flow?
decreases by lowering arterial BP and CO
Which nervous system enhances gastric fluid secretion and motility?
PNS
Emptying of liquids from stomach begins within ? of ingestion- solids begin after ?
1 minute; 15-137 minutes
What is responsible for facilitating the emptying of liquids from the stomach?
contraction of gastric fundus
What is responsible for controlling the emptying of solids from the stomach?
antral contractions
What substances are ABSORBED in the stomach?
highly lipid soluble substances (ETOH, ASA)
What empties faster from the stomach, isoosmolar or hyperosmolar fluids?
iso-osmolar (could drink up to 150 ml of water right before induction)
What condition causes delayed gastric emptying that is not related to fluid type or volume?
diabetic gastroparesis
What medications delay gastric emptying?
opioids, beta agonists, TCA’s, aluminum hydroxide antacid, ETOH, smoking
What causes complete gastric stasis?
TPN
ASA Fasting Guidelines

What is the most abundant lymphocyte?
neutrophils
What do neutrophils do?
seek out, ingest, and kill bacteria- first line of defense (increase in number during infection)
What do eosinophils do?
weak phagocyte- released with foreign proteins like parasites
What do basophils do?
similar to mast cells- contain histamine and heparin- usually active with allergic reactions
What do monocytes do?
precursor to macrophages- contain lysosomes and peroxides, breakdown dead tissue
What cells are in the second line of defense vs third line of defense?
Second- phagocytic WBCs Third- lymphocytes, antibodies
Humoral immunity
production of antibodies by B cells
What do macrophages do?
phagocytosis, destruction- present antigen to helper T cells and secrete cytokines
What do NK cells do?
destruction of virus infected “self” cells and tumor cells, secrete cytokines - do not require antigen stimulation
What do helper T cells do?
secrete cytokines that stimulate helper T cell proliferation and activation of B lymphocytes, cytotoxic T lymphocytes, macrophages
What do cytotoxic T cells do?
engage antigen and secrete perforins into foreign cell, secrete granzymes that destroy target cell
B cells are to ? immunity as T cells are to ? immunity
humoral; cell mediated
Summary of the Immune Response

ABO Blood Groups

Factor I
fibrinogen- forms clot
Factor II
prothrombin
Factor III
tissue factor/thromboplastin
Factor IV
Calcium
vWF
von Willebrand- mediates adhesion
Factor IX
Christmas Factor
Factor XI
fibrin stabilizing factor- crosslinks fibrin
Which factors depend on the presence of Vitamin K for synthesis in the liver?
prothrombin, Factors VII, IX, X
The clotting cascade

which lab value monitors the extrinsic pathway?
PT/INR
Which lab value evaluates the intrinsic pathway?
PTT and ACT
Extrinsic pathway mnemonic
for 37 cents you can purchase the extrinsic pathway
Intrinsic pathway mnemonic
If you can’t buy the intrinsic pathway for 12, you can get it for 11.98
FCP mnemonic
The FCP can be purchased at the 5 and dime for 1 or 2 dollars on the 13th of the month
normal aPTT
25-32 seconds
Normal INR
1.5-2.5
Mediators responsible for procoag, anticoag, and fibrinolytic activities

What is FFP
fluid portion of whole blood that contains clotting factors and inhibitors, source of antithrombin III
What is FFP given for?
dilutional coagulopathy (factors consumed or diluted)- microvascular bleeding, MTP, warfarin reversal, vWF disease, antithrombin III deficiency
Should FFP be cross matched?
whenever possible
What is cryo?
precipitate collected off the top of FFP as it is thawed- contains I, VIII, XIII, vWF, Protein C
What is cryo given for?
microvascular bleeding, vWF disease
What are PRBCs given for?
bleeding, to increase O2 carrying capacity
What blood product can be given unmatched?
platelets
What are platelets used for?
MTP, active bleeding
What is a condition characterized by widespread thrombosis and spontaneous hemorrhage that can be caused by sepsis, incompatible blood transfusion, cancer, or pregnancy?
DIC
What happens in the proximal convoluted tubule?
major reabsorption of nutrients (2/3)
What happens in the loop of Henle?
establishment of osmotic gradient- promotes water reabsorption
Difference between thin descending and thick ascending limbs of the loop of Henle
thin- very permeable to water thick- not very permeable to water- pumps to move solutes
What happens in the DCT?
further adjustments in composition of tubular fluid- combination of secretion and reabsorption
What happens in the collecting duct?
carries tubular fluid through the osmotic gradient in the renal medulla (more fine tuning)
Na in the nephron
reabsorbed along the entire nephron (2/3 within the PCT)
What do the principal cells of the DCT/CD do?
reabsorb Na and H20, excrete K- normally impermeable to water unless acted upon by ADH
What do the alpha intercalated cells of the DCT/CD do?
secrete H (stimulated by aldosterone)- reabsorb K by H-K-ATPase
K in the nephron
2/3 reabsorbed in the PCT, 20% in ascending thick limb, either reabsorbed or secreted in the DCT and CD depending on dietary K intake (fine tuning)
Path of CSF
lateral ventricles -> foramen of Monro -> 3rd ventricle -> aqueduct of Sylvius -> 4th ventricle -> Luschka and Magende -> subarachnoid space -> arachnoid villi and venous drainage
Average cerebral blood flow
50-60 mL/100 g of brain tissue/minute, or 740-900 ml/min
What % of CO does the brain receive?
15%
Regulation of cerebral blood flow

Affect of CBF in response to CO2
increased CO2 leads to increased CBF (CO2+H20–> carbonic acid –> H ions, which lead to vasodilation of cerebral vessels)
Substances that increase the acidity of the brain will do what?
increase H ion concentration, which will increase CBF
What does increased H concentration do to neuronal activity?
depresses it- “CO2 is a great anesthetic”
Affect of oxygen deficiency on CBF
a decrease in cerebral tissue PO2 below 30 mmHg increases cerebral blood flow
CBF is autoregulated between arterial pressure limits of?
around 60-160 (CBF is severely decreased below 50 mmHg)
What happens to the CBF autoregulation curve in patients with chronic HTN?
shifts to the right
CBF Autoregulation

Role of SNS on CBF
when MAP rises quickly, SNS constricts brain arteries to prevent high pressure from reaching small vessels (prevents vascular hemorrhage)
What has a higher metabolic rate, gray or white matter?
gray matter
What is normal CPP (cerebral)?
80-100 mmHg <50= slowing on EEG 25-40= flat EEG sustained <25= irreversible brain damage
Decreases in CPP results in?
cerebral vasodilation (and vice versa)
What does hypothermia do to CMR and CBF?
decreases both
How quickly is CSF formed?
30 mL/hr
What produces CSF?
choroid plexus
What absorbs CSF?
arachnoid villi
C3-5
diagphragm
thumb dermatome
C6
pinky/ulnar dermatome
C8
nipple line dermatome
T4- think cardiac accelerator fibers
groin dermatome
L1
belly button dermatome
T10
knee dermatome
L4-5
xiphoid dermatome
T7
What concentrations are higher in CSF than plasma?
sodium, chloride, magnesium
What concentrations are lower in the CSF than plasma?
potassium, calcium, bicarbonate, glucose
specific gravity and pH of CSF
1.002-1.009; 7.32
Growth hormone releasing hormone (GHRH)
-released from hypothalamus -targets anterior pituitary -causes increased growth hormone secretion
Growth hormone inhibiting hormone (GHIH)
-released from hypothalamus -targets anterior pituitary -causes decreased GH secretion
Thyrotropin releasing hormone (TRH)
-released by hypothalamus -targets anterior pituitary -increases TSH secretion
Corticotoprin releasing hormone (CRH)
-released by hypothalmus -targets anterior pituitary -increases ACTH secretion
gonadotropin-releasing hormone (GnRH)
-released by hypothalamus -targets anterior pituitary -increased secretion of LH and FSH
Prolactin-releasing hormone (PRH)
-released by hypothalamus -targets anterior pituitary -increased prolactin secretion
Prolactin-inhibiting hormone (PIH)
-released by hypothalamus -targets anterior pituitary -decreases prolactin secretion
Antidiuretic hormone (ADH)
-released by posterior pituitary -targets kidneys -increases water reabsorption
Oxytocin
-released by posterior pituitary -targets uterus and mammary glands -increases uterine conractions, increased milk expulsions
Growth hormone/somatotropin
-released by anterior pituitary -targets most tissues -increases growth, amino acid uptake and protein synthesis, glycogen synthesis, blood glucose levels
Thyroid stimulating hormone (TSH)
-released by anterior pituitary -targets thyroid -increases TH secretion
Adrenocorticotropic hormone (ACTH)
-released by anterior pituitary -targets adrenal cortex -increases glucocorticoid hormone secretion
Luteinizing hormone
-released by anterior pituitary -targets ovaries and testes -ovulation and progesterone production in ovaries, testosterone synthesis
Follicle stimulating hormone (FSH)
-released by anterior pituitary -targets follicles in ovaries, seminiferous tubules -follicle maturation and estrogen secretion, sperm cell production
prolactin
-released by anterior pituitary -targets ovaries and mammary glands -milk production, increased response of follicle to LH and FSH
epinephrine
-released by adrenal medulla -targets heart, BV, liver, adipose -increases CO, blood flow to muscles, constriction of BV, increased release of glucose and FA
Mineralcorticoids (aldosterone)
-released by cortex (glomerulosa) -targets kidney -increased Na reabsorption and K/H excretion; enhanced water reabsorption
Glucocorticoids (cortisol)
-released by cortex (fasciculata) -15-30 mg/day (up to 100 in stress) -targets most tissues -increased protein and fat breakdown, increased glucose production, inhibition of immune response and decreased inflammation
Androgens
-released by cortex (reticularis) -targets many tissues -development of secondary sex characteristics
thyroxine (T4) and triidothyronine (T3)
-stimulates oxygen and energy consumption, increases basal metabolic rate -stimulate protein synthesis -t4 is more abundant, T3 is active
calcitonin
-released by thyroid -stimulates osteoblasts, inhibits Ca release from bone -“tones” down calcium
Parathyroid hormone (PTH)
regulates serum calcium, phosphate, and vitamin D synthesis
Glucagon
-released by alpha cells -breakdown of glycogen in liver and muscles -increases glucose level in blood
insulin
-released by beta cells -increases membrane permeability for glucose, lowers glucose level, stimulates synthesis of glycogen in liver and muscles
somatostatin
-released by delta cells -inhibits release of GI hormones
pancreatic polypeptide
self regulate the pancreas secretion activities and affect glycogen levels
What are hormones?
chemical messengers- either proteins, amino acid derivatives, steroids
How are hormones stimulated to be released?
-low blood concentrations -SNS fibers stimulate -hypothalamus secrete stimulating hormones
4 main stimulants for aldosterone release
-hyperkalemia -angiotensin II -hyponatremia -ACTH
Where is cortisol metabolized?
liver
metabolism of epi and NE
metabolized by COMT in the liver and kidney
Metabolism of insulin
can be metabolized by almost all tissues but mostly by liver and kidneys