FAULTS.4

Question 1

SECTIONS ADHERE TO THE KNIFE OR OTHER PARTS OF MICROTOME

1. _______
   - remedy
2. _______
   - remedy
3. _______
   - remedy
4. _______
   - remedy

Answer 1

1. static electrics due to low atmospheric humidity
   - breathe our or blow gently on block and knife to breakup static electricity
2. Knife edge is dirty
   - clean knife edge
3. Knife edge is dull
   - sharpen the knife
4. Knife tilt is too great
   - reduce the tilt

Question 2

RIBBON IS SPLIT OR LENGTHWISE VERTICAL SCRATCHES ARE SEEN ON SECTIONS

1. _______
   - remedy
2. _______
   - remedy
3. _______
   - remedy
4. _______
   - remedy

Answer 2

1. Nicks or damage on knife edge
   - sharpen the knife
2. Dirty embedding
   - re embed in filtered wax
3. Knife edge is dirty
   - clean knife edge with xylene
4. Tilt of knife is too great
   - reduce the tilt

Question 3

SECTIONS ARE LIFTED FROM KNISE ON UPSTROKES
1. _______
- remedy
2. _______
- remedy
3. _______
- remedy

Answer 3

1. knife tilt is too great
   - reduce the tilt
2. Knife is dull
   - sharpen the knife
3. Paraffin is too soft or room temp is warm
   - cool paraffin wax in ice water

Question 4

RESISTANCE IS DELT ON LOWER PART OF SECTION DURING CUTTING

1. Reason
2. Remedy

Answer 4

1. Tilt of knife is too small, paraffin block is therefore compressed against the base of knife
2. Increase the tilt

Question 5

HORIZONTAL/PARALLE
L LINES OR FURROWS ACROSS RHE SECTION (CHATTERS) ARE SEEM, FORMING THIN AND THICK ZONES

1. _______
   - remedy
2. _______
   - remedy

Answer 5

1. knife dye vibrates due to hardness of tissue
   - treat with phenol during processing or collodionizs
2. Tilt of knife is too great
   - reduce the tilt

Question 6

SECTION CUT IS SOMETIMES THIN, SOMETIMES THICK

1. Reason
   - remedy
2. Reason
   - remedy
3. Reason
   - remedy
4. Reason
   - remedy
5. Reason
   - remedy

Answer 6

1. Knife is blunt
   - sharpen the knife
2. Knife is not clamped properly
   - adjust the knife
3. Tilt of knife is too great
   - reduce the tilt
4. Knife or block holder is loose
   - tighten adjusting and locking screws
5. Knife tilt is too small that block is compressed by bevel and section is not cut
   - increase the tilt

Question 7

KNIFE MAKEA HARD METALLIC SCRAPING WHEN CUT

1. Reason
   - remedy
2. Reason
   - remedy
3. Reason
   - remedy

Answer 7

1. tilt of knife is too slanted or too big
   - re adjust the angulation of knife
2. Tissue is too hard
   - take fresh block treated with phenol during processing
3. Knife blade is too thin
   - change the knife