Fatty Acid anabolism 2

Question 1

What is the starting point for fatty acid anabolism?

Answer 1

Acetyl CoA (2C)

Question 2

What is the end point for fatty acid anabolism?

Answer 2

Palmitic acid (16C)

Question 3

What are the 2 difference between Beta oxidation and FA anabolism ( apart from they are in opposite directions)

Answer 3

FA anabolism uses NADPH/NADP+ whilst Beta oxidation uses NAD+/ NADH

FA anabolism uses Malonyl CoA, Beta oxidation uses Acetyl CoA

Question 4

State the equation for Fatty Acid anabolism

Answer 4

Acetyl CoA + ATP+ HCO3- -> Malonyl CoA + ADP + Pi+ H+

Question 5

What is the size of Fatty Acid Synthase?
How many active sites?
What functions does it have?

Answer 5

272 kDa
6 active sites
Modifying and condensing

Question 6

What is the 1st step in FA anabolism?

Answer 6

MAT = Malonyl/Acetyl CoA-ACP Transacylase
KS = β Ketoacyl-ACP Synthase 

Acetyl group is transferred to KS via ACP and MAT
Malonyl transferred into ACP by MAT
KS joins the acetyl and Malonyl CAUSING A LOSS OF CO2

Question 7

What is the equation for the first step of FA anabolism?

Answer 7

Acetyl-Ks + Malonyl-ACP -> Acetoacetyl-ACP + CO2

w/KS

Question 8

How many steps are in Fatty Acid anabolism

And how many cycles are there?

Answer 8

4 steps

Cycle repeated 6x

Question 9

Steps 2-4 are what reactions?

Answer 9

Step 2- Reduction
Step 3- Dehydration
Step 4- Reduction

Question 10

What enzyme releases the FA after anabolism?

Answer 10

thioesterase

Question 11

What happens to the lipids that are made?

Answer 11

In an anabolic state the FA are converted to TG and stored

If synthesized in the liver, lipoproteins are used to export TG to adipose and other tissues

Question 12

What is hyperlipasemia?

Answer 12

Defect in LDL-R gene so high plasma LDL.

Homozygous