FA piruvatas (complex) 12/22 Flashcards
PDH (pyruvate dehydrogenase complex) located where?
in mitochondria
what links PDH?
Glycolysis and TCA cycle
is regulation of PDH is same in fed and fasting states?
no. In fed state - active; in fasting - inactive
what is net reaction?
Pyruvate+NAD+CoA –> acetylCoa+CO2+NADH
What 5 cofactors for PDH?
Thiamine phosphate (B1) FAD (B2) NAD (B3) CoA (B5) Lipoic acid
What activates PDH? 3
incr. NAD/NADH ratio (cia sitas dalykas inc. in alcohol)
incr. ADP
inc. Ca2+
What inhibits PDH? 3
ATP;
AcetylCoA
NADH
incr. NAD/NADH ratio on PDH?
activates
incr. ADP on PDH?
activates
inc. Ca2+ on PDH?
activates
ATP on PDH?
Inhibits
AcetylCoA on PDH?
Inhibits
NADH on PDH?
Inhibits
what does arsenic?
inhibits lipoic acid
PDH deficiency. Age?
infancy
PDH deficiency. Manifestation?
neurologic defect, lactic acidosis, inc. serum alanine
PDH deficiency. mechanism?
X-linked; causes buildup of pyruvate that gets shunted to lactate (via LDH) and alanine (via ALT).
Alanine aminotransferase. What vitamin?
B6
Alanine function?
alanine carries amino groups to the liver from muscle (cahill cycle)
Pyruvate carboxylase, what vitamin?
biotin (B7)
OAA functions?2
can replenish TCA cycle or be used in gluconeogenesis
Pyruvate dehydrogenase, what vitamins?
B1, 2, 3, 5, lipoic acid
Pyruvate dehydrogenase, function?
transition from glycolysis to TCA cycle
Lactic acid dehydrogenase, what vitamin?
B3
