Experiments for Memory Molecules

Question 1

CamKII KO

Answer 1

Morris water maze

• if visible platform = imparied but learn eventually
• if hidden platform =
  
  • severly impaired, never learn
  • don’t search in target audrant specifically

Question 2

Fear conditining and CamKII

Answer 2

CamKII is shown to be phosphorylated during fear conditionning

Use of KN-62 = inhibits phosphorylation of CamKII

• decrease of freezing in contextual and tone fear

Question 3

CREB and fear conditionning

Answer 3

Mice genetically engineered to repressed cREB

• at 3h same freezing
• 24hh: significant decrease of freezing

CREB is implicated in long phase LTP, so consistent here where long term memory is impaired.

Question 4

CREB and WM

Answer 4

Infuse with antisense that blocks CREB

• learn fine
• recollection after 3 days is imparied
  
  • confirm that CREB is for long term

Question 5

CREB activation in CA1

Answer 5

Look for CREB in CA1

• native = nothing
• unpaired tone = nothing
• paired with shock = +++

Question 6

ARC and memory

Answer 6

Rat is exploring a novel environment and they track ARc at different times afer

• caged animal= no arc
• after exploration = arc in the nucleus
• 20min later= arc is in the cytoplasm