Experiments for LTP2

Question 1

Fear Conditionning Experiments

Answer 1

Stimulate in the medial geniculate and record in lateral amygdala

Electrical stim - HFS - electrical epsp = increase response for same stim = LTP

Auditory epsp - HFS - ausi epsp = increase response = LTP

Parralel mechanisms

Question 2

Food pellet training

Answer 2

Rat trained to retrieve food from right hand. Removed brain, stimulated and recorded

Motor learning is controlateral

Control; response is the same for trained and untrained hemisphere

Trained rats= response is bigger for trained (left) hemisphere

Question 3

NMDAR antagonist AP-5 and LTP

Answer 3

When the slice is treated zith AP5, LTP cannot be induced.

The response for a bigger stimuation is bigger, but then it goes back to the same level when weak stimuation

Question 4

AP5 and Morris WM

Answer 4

Gave AP5 or vehicule and then trained them to find hidden platform

Rat with AP5 didn’t learn, less time searching in target quadrant when probe

Trained animals and then gave them AP5

Rats learned how to find the platform

• NMDAR - LTP - Learning = related
• NMDAR - induction of LTP only

Question 5

Doogie mice

Answer 5

Add 1 gene to increase NMDAR

• Slice exp show enhaced LTP
• Behav exp show improvment, not huge but present
  
  • after may training, the result is the same for all animals

Question 6

Dissociation of LTP and learning

Answer 6

ACPD is a mGlutR agonist that blocks LTP

When animal are treated with ACPD it _doesn’t impair learning _