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CLINICAL CHEMISTRY > EXPERIEMT #14 FINALS > Flashcards

EXPERIEMT #14 FINALS Flashcards

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1
Q

What are we talking about in experiment 14?

A

Albumin
Total serum proteins
A/G ratio

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2
Q

How are you going to correlate test results with pathologic conditions?

A

Electrophoresis pattern

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3
Q

What are you going to explain in Experiment #14?

A

Amino acid synthesis and metabolism

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4
Q

What are you going to discuss in Experiment #14?

A

The different types of aminoacidopathies

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5
Q

In Serum Albumin Determination, what method is used?

A

Bromocresol Green Method (BCG)

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6
Q

What is the principle of Serum Albumin Determination?

A

The binding behavior of the protein with an anionic dye.

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7
Q

Principle of Serum Albumin Determination:

Determination of __________ in ____________is usually based on the ___________________, _____________ forming a ____________.

A

Albumin
serum or plasma
binding behavior of the protein with an anionic dye
bromcresol green
green colored complex.

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8
Q

What is the concentration standard of Total protein?

A

6g/dL

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9
Q

What is the concentration standard of Serum Albumin?

A

4g/dL

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10
Q

What is the formula for computing Total protein and serum standard?

A

Cu= Au/Astd x Cstd

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11
Q

How much serum albumin reagent is added?

A

3.0mL

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12
Q

To which tube is serum albumin reagent added?

A

Blank
Standard
Sample

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13
Q

How much protein standard is added?

A

3.0mL

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14
Q

To which tube protein reagent is added?

A

Blank
Standard
Sample

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15
Q

How much and to which tube water is added for both serum and Albumin determination?

A

20uL
Blank

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16
Q

How much and to which tube STANDARD is added for both serum albumin and total protein determination?

A

20uL
Standard sample

17
Q

How much and to which tube SERUM is added for both serum Albumin and total protein determination?

A

20uL
Sample

18
Q

In performing Albumin, total protein determination, what specimen is used?

A

Serum

19
Q

In SAD and Total Protein, at what temperature are we going to stand the samples?

A

Room temperature

20
Q

Under SAD, at what absorbance are we going to read the blank, standard and sample?

A

600nm

21
Q

Under SAD, how much time is its stability?

A

60 MINUTES / 1 HR

22
Q

In determination of Total Proteins, what method is used?

A

Biuret (Modified)

23
Q

What is the principle of Biuret Method?

A

The copper binds to the peptide bond structure of the protein.

24
Q

When a solution containing proteins is rejected with a tartrate-complex cupric ions and
alkali, the copper binds to the peptide bond structure of the proteins. What is the color that is produced?

A

Purple colored chromogen

25
Q

When a solution containing _________ is _________with a ______________ and ________, the copper binds to the peptide bond structure of the proteins, forming a purple colored chromogen.

A

proteins
rejected
tartrate-complex cupric ions
alkali

26
Q

Under SAD, stand the samples at room temperature for?

A

1 minute

27
Q

Under Total Protein, stand the samples at room temperature for?

A

5 minutes

28
Q

TRUE OR FLASE:

Inverting the tubes is necessary in performing SAD and Total Albumin determination.

A

TRUE

29
Q

What is the formula in determination of Globulin?

A

Globulin = gms. of total protein - gms. of albumin

30
Q

What is the formula in determination of A/G Ratio?

A

A/G ratio = gms, of Albumin / gms. of Globulin

31
Q

What do you call the procedure used in SAD and Total Albumin determination?

A

Colorimetric Procedure

32
Q

What is the normal value of TOTAL PROTEIN in Conventional and S.I unit?

A

6 - 8
60 - 80

33
Q

What is the normal value of ALBUMIN in Conventional and S.I unit?

A

3.5 - 5
35 - 50

34
Q

What is the normal value of GLOBULIN in Conventional and S.I unit?

A

1.8 - 3.2
18 - 32

35
Q

What is the normal value of A/G RATIO in Conventional?

A

1.5-2.4 : 1

36
Q

What is the color of the complex formed when albumin in serum or plasma binds with the anionic dye, bromcresol green, during the determination of albumin?

A

Green colored complex

CLINICAL CHEMISTRY (13 decks)

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