EXPERIEMT #14 FINALS Flashcards
What are we talking about in experiment 14?
Albumin
Total serum proteins
A/G ratio
How are you going to correlate test results with pathologic conditions?
Electrophoresis pattern
What are you going to explain in Experiment #14?
Amino acid synthesis and metabolism
What are you going to discuss in Experiment #14?
The different types of aminoacidopathies
In Serum Albumin Determination, what method is used?
Bromocresol Green Method (BCG)
What is the principle of Serum Albumin Determination?
The binding behavior of the protein with an anionic dye.
Principle of Serum Albumin Determination:
Determination of __________ in ____________is usually based on the ___________________, _____________ forming a ____________.
Albumin
serum or plasma
binding behavior of the protein with an anionic dye
bromcresol green
green colored complex.
What is the concentration standard of Total protein?
6g/dL
What is the concentration standard of Serum Albumin?
4g/dL
What is the formula for computing Total protein and serum standard?
Cu= Au/Astd x Cstd
How much serum albumin reagent is added?
3.0mL
To which tube is serum albumin reagent added?
Blank
Standard
Sample
How much protein standard is added?
3.0mL
To which tube protein reagent is added?
Blank
Standard
Sample
How much and to which tube water is added for both serum and Albumin determination?
20uL
Blank
How much and to which tube STANDARD is added for both serum albumin and total protein determination?
20uL
Standard sample
How much and to which tube SERUM is added for both serum Albumin and total protein determination?
20uL
Sample
In performing Albumin, total protein determination, what specimen is used?
Serum
In SAD and Total Protein, at what temperature are we going to stand the samples?
Room temperature
Under SAD, at what absorbance are we going to read the blank, standard and sample?
600nm
Under SAD, how much time is its stability?
60 MINUTES / 1 HR
In determination of Total Proteins, what method is used?
Biuret (Modified)
What is the principle of Biuret Method?
The copper binds to the peptide bond structure of the protein.
When a solution containing proteins is rejected with a tartrate-complex cupric ions and
alkali, the copper binds to the peptide bond structure of the proteins. What is the color that is produced?
Purple colored chromogen
When a solution containing _________ is _________with a ______________ and ________, the copper binds to the peptide bond structure of the proteins, forming a purple colored chromogen.
proteins
rejected
tartrate-complex cupric ions
alkali
Under SAD, stand the samples at room temperature for?
1 minute
Under Total Protein, stand the samples at room temperature for?
5 minutes
TRUE OR FLASE:
Inverting the tubes is necessary in performing SAD and Total Albumin determination.
TRUE
What is the formula in determination of Globulin?
Globulin = gms. of total protein - gms. of albumin
What is the formula in determination of A/G Ratio?
A/G ratio = gms, of Albumin / gms. of Globulin
What do you call the procedure used in SAD and Total Albumin determination?
Colorimetric Procedure
What is the normal value of TOTAL PROTEIN in Conventional and S.I unit?
6 - 8
60 - 80
What is the normal value of ALBUMIN in Conventional and S.I unit?
3.5 - 5
35 - 50
What is the normal value of GLOBULIN in Conventional and S.I unit?
1.8 - 3.2
18 - 32
What is the normal value of A/G RATIO in Conventional?
1.5-2.4 : 1
What is the color of the complex formed when albumin in serum or plasma binds with the anionic dye, bromcresol green, during the determination of albumin?
Green colored complex
