exp 3.7 use starch agar yo show digestive activity during gemination

Question 1

what is the nutrient

Answer 1

starch

Question 2

what is the medium

Answer 2

agar

Question 3

what agar is used

Answer 3

starch agar

Question 4

what seeds are used

Answer 4

broad bean seeds

Question 5

why are the seeds soaked in water

Answer 5

to soften the testa

Question 6

what is the control

Answer 6

boiled seeds

Question 7

how are seeds placed in petri dish

Answer 7

cut in half, face down

Question 8

where is the petri dish left?

Answer 8

incubator at 20-30 degrees

Question 9

how long are seeds left in incubator

Answer 9

2 days

Question 10

why is the incubator at 20-30 degrees

Answer 10

optimum temperature for enzyme activity

Question 11

aspetic techniques used

Answer 11

seeds soaked in disinfectant
petri dish opened minimally for a short period of time

Question 12

sterile techniques used

Answer 12

forceps are flamed

Question 13

what is used to test for the presence of starch

Answer 13

iodine

Question 14

what happens when iodine is added to the petri dish

Answer 14

the plate will turn blue/black except for where the seeds were placed which will remain yellow proving that germination has occurred

Question 15

what happens when iodine is added to a plate with boiled seeds

Answer 15

whole plate turns blue/black

Question 16

name the enzyme, substrate and product

Answer 16

starch = substrate
amylase = enzyme
maltose = product