exp 3.7 use starch agar yo show digestive activity during gemination Flashcards
what is the nutrient
starch
what is the medium
agar
what agar is used
starch agar
what seeds are used
broad bean seeds
why are the seeds soaked in water
to soften the testa
what is the control
boiled seeds
how are seeds placed in petri dish
cut in half, face down
where is the petri dish left?
incubator at 20-30 degrees
how long are seeds left in incubator
2 days
why is the incubator at 20-30 degrees
optimum temperature for enzyme activity
aspetic techniques used
seeds soaked in disinfectant
petri dish opened minimally for a short period of time
sterile techniques used
forceps are flamed
what is used to test for the presence of starch
iodine
what happens when iodine is added to the petri dish
the plate will turn blue/black except for where the seeds were placed which will remain yellow proving that germination has occurred
what happens when iodine is added to a plate with boiled seeds
whole plate turns blue/black