exp 2.8 investigate the influence of light intensity on the rate of photosynthesis Flashcards
what is a suitable underwater plant for this experiement
fresh elodea
why do we use an underwater plant?
so we can see oxygen bubbles in the water
Method
cut the stem of the elodea
hook a paper clip onto the end of the elodea + drop it stem up into the boiling tube of pond water
place boiling tube into a water bath at 25 degrees
switch on light source and place the water bath containing elodea away from lamp and wait 5 min
observe bubbles being released. count and record the number of bubbles released per minute
repeat varying the distance of the lamp and the plant
why do we hook a paper clip onto the elodea
holds the plant at the bottom of the boiling tube so we can see the bubbles rise up
how do we ensure maximun concentration of Co2 in the water
1% w/v sodium hydrogen carbonate is in water
what is the constant in this experiment
CO2
what is the variable in this exp
light intensity
why do we wait 5 min after adjusting the light intensity
allows the plant to adjust to the new light intensity
why does the water bath have to be at 25 degrees
dark stage requires enzymes and this is the optimum temperature for enzyme activity
how do we measure the rate of photosynthesis
bubbles produced per minute
more bubbles=more photosynthesis
what is the formula for the light intensity
1 divided by distance in cm
draw the graph
y axis= rate of photosynthesis
x axis = light intensity
L shape on its side kinda
explain the graph
as light intensity increases, the plant absorbs more light and the rate of photosynthesis increases
the plant becomes light saturated ie it cannot absorb more light and the rate remains the same