exp 2.5 to investigate the effect of temperature on enzyme activity Flashcards
1
Q
substrate
A
hydrogen peroxide
2
Q
enzyme
A
catalase
3
Q
source of enzyme
A
celery
4
Q
variable
A
temperatureh
5
Q
how do we vary the temperature
A
various water baths of different temperatures
6
Q
constant
A
pH
7
Q
how do we keep the pH constant
A
pH buffer or 7-9
8
Q
product of this exp
A
water and oxygen
9
Q
after what temperature are enzymes denatured
A
60 degrees celcius
10
Q
graph your results
A
y axis- rate of enzyme activity
x axis - temperature
nice curve- peaks at around 25-30 and drops by 60
11
Q
draw the diagram for this experiment
A
same as all effect of pH
12
Q
how do we measure the rate of enzyme activity
A
foam produced per unit time
