Exp 3.1 to investigate the growth of leaf yeast using agar plates

Question 1

aseptic techniques

Answer 1

minimising unwanted organisms

Question 2

sterile techniques

Answer 2

all microorganisms destroyed

Question 3

what are some aseptic techniques

Answer 3

placing base of open plate bottom side up
making sure that the leaf plate is only opened for a short time which reduces contamination from air
sealing plates so they cannot be opened accidentally

Question 4

what are some sterile techniques

Answer 4

flaming, swabbing with disinfectant and immersion in liquid disinfectant

Question 5

is washing hands aseptic or sterile

Answer 5

aseptic

Question 6

is washing bench with disinfectant aseptic or sterile

Answer 6

aseptic

Question 7

is flaming forceps aseptic or sterile

Answer 7

sterile

Question 8

why do we handle the leaf with forceps only

Answer 8

prevents microbes from your hands getting on to leaf

Question 9

why do we open the petri dish minimally for a minimum amount of time

Answer 9

prevents microbes from air entering petri dish

Question 10

why does the leaf not touch the agar

Answer 10

prevents microbes that may be on leaf from growing on the agar

Question 11

why does the bottom of leaf face the agar

Answer 11

more yeast on this side as cuticle on upper leaf prevents yeast from being released

Question 12

why are the agar plates sealed

Answer 12

prevents reopening of petri dish unnecessarily

Question 13

why do we label the lid of the petri dish

Answer 13

this allows the agar to viewed without obstruction

Question 14

result of this exp

Answer 14

pink colonies form after a few days - yeast