EXAM I TISSUE SLIDES; ENJOY! :) Flashcards
Identify tissue type & location
What type of cells are in this tissue (white cells)?
- Epithelium
- Simple Columnar
- Ileum
- Goblet cells
ID: Tissue Type & Location
- Epithelium
- Simple cuboidal
- Kidney
ID Tissue Type & Location, There are two compartments here, what are they?
- Epithelium and Connective Tissue (left portion)
- Non-keratinized Stratified Squamous
ID Tissue Type & Location. What is the bottom portion? What type of stain is used?
- Epithelium
- Transitional (dome-shaped cells)
- Ureter
- Connective tissue (bottom)
- H&E Stain (Hematoxylin stains nuclei dark purple, Cytoplasm is stained pink)
ID Tissue Type
- CT
- Dense Bone
ID Tissue Type
- CT
- Spongy/Cancellous Bone
ID Tissue Type
- CT
- Adipose
ID Tissue Type
- CT
- Hyaline Cartilage
ID Tissue Type
- Muscle Tissue
- Skeletal Muscle
ID Tissue Type
- Muscle Tissue
- Skeletal Muscle
ID Tissue Type
- Muscle Tissue
- Cardiac Muscle
ID Tissue Type & Location
- Muscle Tissue
- Smooth Muscle
ID Tissue Type & Location; What are the lines pointing to?
- Nervous Tissue
- Cerebral Cortex
- Labeled: Neurons
ID Tissue Type & Location
- Nervous Tissue
- Purkinje Cell
ID Tissue Type & Location, what is the arrow pointing to?
- Kideny
- Label: lumen
- Collecting duct
ID Tissue Type & Location
- Kidney
- Proximal Convoluted Tubules
- Simple Cuboidal
What type of stain is used here?
- pH Staining on Paramecium
- Fast green at pH 2.5
- This just shows what different pH demonstrates using same staining technique
What type of stain is used here?
- pH Staining on Paramecium
- Fast green at pH 8.0
- This just shows what different pH demonstrates using same staining technique
- What type of tissue is this?
- What type of staining technique?
- Adipose tissue (low mag. 100X)
- Osmium Tetroxide
What type of tissue? What staining technique is used?
- Adipose tissue (high mag. 400X)
- Osmium Tetroxide
- What type of tissue?
- Staining technique used and what organelle is stained?
- Liver
- Schiff reagent
- Stains nuclei pink (reacts with aldehyde groups)
- What type of tissue?
- Staining technique?
- Liver
- PAS staining (Periodic acid-Schiff)
- Arrow pointing at glycogen deposits - deep pink
- Type of tissue?
- Staining technique?
- What is stained here?
- Liver
- Best Carmine
- Stains glycogen deposits - dark pink dots
- Tissue type?
- Stain?
- Liver
- Best Carmine
- Stains glycogen - dark pink (not the purple)
- Tissue type?
- Stain?
- Liver
- RNA stained with pyronin methyl green
- Basic dye (DNA & RNA are basophilic)
Tissue?
Stain?
- Pancreas
- Immunocytochemical Stain
- Anti-glucagon (alpha cells more centered)
- Direct labeling of Antibody conjugated with fluorescent dye
ID Tissue Type
Stain?
- Pancreas
- Immunocytochemical Stain
- Anti-Insulin (beta cells more peripheral than glucagon)
Tissue?
Stain?
- Pituitary
- Immunocytochemical
- Anti-ACTH
Tissue?
Stain?
- Tonsil
- Immunocytochemical Stain
- Anti-IgG
Tissue type?
- Stratified squamous lightly keratinized
- Skin
- What is labeled?
- Tissue type?
- Desmosomes
- Stratified squamous epithelium
- Epidermis
Tissue type?
Simple Squamous
Tissue type?
What are the clear cells called?
- Pseudostratified columnar epithelium
- Goblet cells
- Note the multiple layers of nuclei found at different levels in a single layer of cells
Tissue type?
Where is this tissue located?
- Transitional Epithelium (notice the dome shaped cells)
- Ureter; Urothelium
Tissue type?
Location?
- Simple cuboidal
- Kidney
Tissue type?
Stratified squamous non-keratinized
Tissue type?
Location?
- Simple cuboidal
- Kidney
Tissue type?
- Simple Columnar
- Note the microvilli
Tissue type? Location?
- Transitional
- Urothelium
- Urinary tract
Tissue type?
Location?
- Columnar (notice the brush border)
- GI tract
Tissue type? Location?
Simple cuboidal; Kidney
Tissue type? Location?
- Transitional (notice the dome shaped cells)
- Urothelium; Urinary tract
Tissue type?
Simple cuboidal
Tissue type?
- Simple columnar
- Notice the uniform lining, making it simple (circled)
Tissue type?
- Transitional
- Urothelium
Tissue type?S
- Simple columnar
- Notice the goblet cells; which are usually in intestinal epithelium = columnar
Tissue type?
- Pseudostratified with microvilli
Tissue type?
- Stratified columnar
Tissue type?
Non-keratinized stratified squamous
What type of gland and what is occurring at the arrow?
- Sebaceous gland
- Cells are breaking down to release their product (thick, oily material)
Type of gland?
What is circled?
- Sublingual gland (contain mucous acini ONLY)
- Duct
- Mucous acini are pale in appearance = PAS+
- Contain Myoepithelial cells (surround acini)
Type of gland?
What are the arrows pointing to?
- Parotid gland; Serous acini ONLY
- Apical region = zymogen granules
- Basal region = RER
Tissue Type
Unilocular Adipose Tissue 400x
Tissue type?
What are the arrows pointing to?
- Submandibular gland (BOTH serous and mucous cells, and myoepithelial cells)
- Top left = ducts
- Bottom left = serous acini
- Botton right = mucous acini
Tissue Types?
Major Is Unilocular Adipose Tissue 100x
bottom Left corner displays Fibrous Connective Tissue
Tissue Type
Elastic Connective Tissue xs, 100x
Tissue Type
Elastic Connective Tissue Is, 100x
Stains pink w/ H and E
Tissue Type
Areolar CT 400X
Mostly dark lines = Elastic Fiber
background includes collagen fibers if look carefully
- Type of gland?
- Label arrows
- Parotid gland
- Left arrow = Connective tissue
- Right arrow = serous acini
Tissue Type
Areolar Connective Tissue
see elastic fibers
background would include collagen fibers but not see well
Gland type?
Parotid gland
Type of gland?
Label arrows
- Sublingual gland
- Left arrow = striated duct
- right arrow = mucous acini
Tissue Type
White Fibrous Connective Tissue 100x
Better slide of Dense Regular Connective Tissue
see parallel and regular distribution
see very thin rows of nuclei
Gland type?
- Sublingual gland
- Note that nuclei are located around the bases of the mucous acini
Gland type?
Label arrows
- Sublingual gland
- top left = mucous acini with basal nuclei
- bottom left = serous acini
- disregard right arrow
Tissue Type
Dense Regular Connective Tissue, 400x
Tissue Type
Plasma Cells
peripheral distribution of lumps of DNA showing basically through a clock
Gland type? Label arrows
- Sublingual gland (400X)
- Top = secretory acini
- Middle = myoepithelial cells
- Bottom = intralobular duct; striated duct (smaller diameter than Interlobular duct)
Type of gland?
Sublingual gland
Type of gland? Label arrows
- Submandibular gland
- Left = serous acini
- Right = Interlobular duct; simple cuboidal duct (larger within the CT and larger diameter than serous acini)
Tissue Type
Plasma cells
clock-like representation of DNA
all in delicate connective tissue
on either side is a simple columnar epithelial tissues
In the Gut
ID what used to take slide
What slide is depicting
and the 3 labels of cell structures (A, B, C)
Plasma Cell
TEM slide
A. Rough Endoplasmic Reticulum containing immunoglobulin molecules
B. Cartwheel Nucleus
C. Region of the Golgi Apparatus
Type of gland?
Label arrows
- Submandibular
- Left = striated duct
- Right = serous acini
Gland type?
Label arrows
- Parotid gland
- Left = Interlobular septum (in between CT partitions)
- Right = Interlobular duct (INTER = between CT)
- The mucous acini looking cells are actually adipose cells = older patient
ID Tissue Type and 4 labeled structures
Plasma Cell
A. Golgi Region
B. Cartwheel Nucleus
C. Rough Endoplasmic Reticulum
D. Nucleus of a Fibroblast
Type of gland?
- Parotid
- Notice it is surrounded by CT; Interlobular duct
ID
TEM of a Mast cell
Gland type? What is the arrow pointing at?
- Submandibular gland
- Blood vessels surrounding duct
ID slide
Stain: Toluidine Blue
Dark Puple sctructures = Mast Cells
Nuclei stain lightly in Mast cells
A = Muscle Cell
below mast cells is Fibrouc Connective tissue
Gland type?
Submandibular gland
Gland type?
Submandibular gland
Gland type?
Submandibular gland
Gland type?
- Mammary gland
- No secretory units, therefore inactive gland
Gland type?
- Mammary gland
- No secretory units = inactive
ID
TEM of a Mast Cell with cytoplasmic granules of different density
ID the labeled structures
A. Mast Cell with metachromatic granules in the Cytoplasm
B. Blood Vessel
Stain: Toluidine Blue
ID
Macrophages
Stain with India Ink (very black ink made with carbon fibers)
They digest the ink so it makes them easy to find
Dark spots = undigestable India Ink = inside Macrophage
ID
Macrophages
Stain with India Ink (very black ink made with carbon fibers)
They digest the ink so it makes them easy to find
Dark spots = undigestable India Ink = inside Macrophage
ID
Machrophage
Stain: Toluidine Blue
Fiber in Background
Yellow arrow : Fibroblasts
Red Arrows : cells w/ clear spots are fibrocytic vesicles and shows phagocytosis
A and B. Macrophages
C. Fibroblast
D. Collagen
ID slide and labeled structures
TEM of Marcrophage
A. Nucleus
B. Mitochondria
C. Vesicle
D. Secondary lysosome
E. Primary lysosome
F. Filopodia
Lots of vesicles and process that stain the cell.. Acidic, Lots of Mitochondria Activity
ID
Fibrocytes
see some semblance of the fiber cells but fibrocytes pick up most of the stain
ID
Fibrocytes
A. Collagen fibers in cross-section
B. Fibroblast in active site
c. collagen fibers in longitudinal section
ID
Adipose 100x
Unilocular
Fibrous connective tissue in the corner
ID
White Fat
Unilocular Adipose Tissue
When can be seen…nuclei pushed to side
cells empty due to process used to preserve tissue (alcohol dissolve)
ID
Brown Fat
Multilocular Adipose Tissue
has lots of mitochondria
ID
Tendon and Muscle 400x
Tendon not stretched
still displays the dense compactness
ID
Reticular
Silver Stain
Found in Lymph node in the spleen
Dense irregular CT with predominance of reticular fibers
ID
Elastic Connective Tissue
Arrows to Squiggly = Lamellae
Dense type of irregular CT with an adundance of elastic fibers which form discontinuous lamellae
ID Tissue and labeled structures
Dense Irregular Adult Connective Tissue
No parallel organization of Collagen bundles but densely packed
thicker bundles of collagen than in Loose CT
found in fascia, submucosa of GI tract, and in dermis of integument
A. Collagen Fibers
B. Blood Vessels
C. Connective Tissue cell nuclei
D. collagen Fibers
ID
White Fibrous Connective Tissue
(Dense Regular CT) 100x
bundles of collagen fibers
relatively unstained
nuclei squeezed between and see parallel organization
ID
Dense Regular Adult CT
high ratio of fibrous components to fibroblasts
thicker bundles of collagen than in loose CT
highly ordered bundles of collagen separated by single rows of fibroblasts
found in ligaments and tendons
Nuclei compressed b/w bundles (very elongated and thin)
everything runs parallel
ID
Areolar (Loose Adult) Connective Tissue 100x
Collagen not really noticable here
high ratio fibroblast to fibrous components
Type I collagen and elastic fibers
usually found beneath epithelial tissues of most organs
contains fibroblasts, mast cells, macrophages, and capillaries*
****no vascular tissue in epithelial but YES in CT*****
ID
Adult Loose (Areolar) Connective Tissue
Collagen not really noticable here
high ratio fibroblast to fibrous components
Type I collagen and elastic fibers
usually found beneath epithelial tissues of most organs
contains fibroblasts, mast cells, macrophages, and capillaries*
****no vascular tissue in epithelial but YES in CT*****
ID
Embryonic Connective Tissue
can see fibers but thin and spider-web like
found in umbilical cord and in pulp of developing teeth
“Wharton’s” Jelly in umbilical cord
composed of some collagen and elastic fibers but mostly an abundance of extracellular matrix (usually in the form of gylcoprotein and proteoglycans)
ID
Umbilical Cord; x.s.
A. Wharton’s Jelly = Embryonic CT and the nondescript area
B. Placental Artery
C. Allantois
D. Placental Artery
E. Placental Vein
ID
Unilocular Adipose Tissue
ID tissue and labels
Adult Connective Tissue: Elastic Connective Tissue
A. Elastic Fibers
B. Smooth Muscle Cells
ID
Reticular Tissue
Adult -type connective tissue
characteristic of lymphatic tissue
silver stain
ID tissue and labels
Dense Regular Connective Tissue
Adult Connective Tissue
found in ligaments and tendons
A. Skeletal Muscle
B. Regularly arranged collagen bundles
C. Fibrocyte compressed by the aligned collagen bodies
ID Tissue and labels
Dense Irregular Adult Copnnective Tissue
found in dermis of the skin, submucosa of GT tract, and other sites
Fibroblasts are spare separated by collagen bundles, and recognized by their oval nucelus
A. Blood capillary
B. Oval nucleus of a fibroblast
C. Collagen bundles thick, wavy, and irregularly arranged
ID Tissue and Labels
Loose (Areolar) Adult Connective Tissue
abundant elastic fibers and collagen bundles embedded in the gorund substance
A. Oval nucleus of a Fibroblast
B. Elastic Fibers are thin, straight, and branching
C. Collagen bundles are thick and wavy
ID Tissue and labels
Embryonic Connective Tissue
contains abundant extracellular matric rich in proteoglycand
collagen and reticular fibers are present but nt abundant
A. Nucleus of a mesenchymal cell embedded in the extracellular matrix
B. Amnion
Lymph Node, Cortex; 40x
Lymph Node, Cortex; 40x
Lymph Node, Cortex; 100x
Lymph Node, Cortex; 100x
Lymph Node, Cortex; 100x
Lymph Node, Cortex; 100x
Lymph Node, Medulla; 40x
Lymph Node, Medulla; 40x
Lymph Node, Medulla; 100x
Lymph Node, Medulla; 100x
Anthracosis Lymph Node; 100x
Lymph Node, Cortex; 40x
Thymus, 40x
Thymus; 100x
Spleen; 40x
Spleen; 40x
Spleen; 40x
Spleen; 100x
Spleen; 100x
Spleen; 100x
Spleen; 100x
Scalp, (thin skin); 40x
Scalp (thin skin); 400x
Thick skin; 100x
Integument, Dermis; 100x
Papillary Layer - capillaries, elastic fibers
Reticular Layer - fibrocytes, MOs, adipocytes
Sebaceous Gland; 100x
Hair follicle
Holocrine excretion
Scalp, Horiz; 100x
Meissner’s Corupuscle; 100x
Top: Corneum layer
Bottom: Meissner’s Corpuscle
Meissner’s Corpuscle; 400x
Pacini Corpuscle; 40x
Pacini Corpuscle; 100x
Purkinje Fibers; 400x
Beneath endocardium lining IV septum, stain lighter
Carry impulses to ordinary cardiomyocytes
Aorta; 40x
Muscular Artery; 100x
Name the two structures
Trachea; 100x
Lung with Bronchiole; 40x
Lung; 100x
Lung; 400x
Alveolus; 1000x
Lung; 100x
Renal Cortex, 100x
Bowman’s capsule, 100x
Bowman’s capsule, 400x
Bowman’s capsule, 400x
Glomerulus, 100x
Glomerulus, 400x
Glomerulus, 100x
Glomerulus
Human Kidney, 100x
Human Kidney, 100x
Human kidney, 400x
Human kidney, 400x
DCT
Human kidney, 400x
PCT
Human kidney, 400x
Human ureter, 40x
Ureter, 100x
Transitional Epithelium
Human ureter, 100x
Transitional Epithelium; Urothelium
Ureter, 400x
Transitional Epithelium; Urothelium
Bladder; 40x
Bladder, 100x
Bladder, 400x
Transitional epithelium; Urothelium
Trachea
Fundic Stomach, 40x
Fundic Stomach, 100x
Fundic Stomach, 400x
Duodenum, 100x
Duodenum, 100x
Duodenum, 100x
Jejunum, 100x
Jejunum, 100x
Jejunum, 400x
Jejunum, 400x
Ileum, 40x
Ileum, 100x
Ileum, 100x
Ileum, 400x
