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Micro 425 > Exam 3 (Misc Gram- Bacilli/Coccobacilli; Panther System) > Flashcards

Exam 3 (Misc Gram- Bacilli/Coccobacilli; Panther System) Flashcards

1
Q

Stool specimens suspected of being Vibrio should be transported in this type of media:

A

Cary-Blair

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2
Q

What selective media is used for Vibrio:

A

TCBS (thiosulfate citrate bile salts)

  • bile salts inhibit gram+
  • high pH inhibits other intestinal flora
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3
Q

Which serotypes of Vibrio cholerae have been involved in epidemics/pandemics:

A

O1

O139

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4
Q

Vibrio cholerae is strongly linked to:

A

contaminated seafood (water exposed to feces)

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5
Q

Rice water stools are associated with:

A

Cholera

Vibrio cholerae

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6
Q

These two organisms are found in brackish or saltwater:

A

Vibrio vulnificus

Vibrio parahaemolyticus

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7
Q

All clinical cases of ______ are to be reported to state health depts which report annually to CDC:

A

Vibrio spp.

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8
Q

What differentiates Vibrio vulnificus from cholerae symptomwise:

A

V. vulnificus pathogenesis extends beyond GI tract

*septicemia, septic shock, wound infections, amputations

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9
Q

Why would Vibrio vulnificus cause a need for limb amputation:

A

Toxins released in infected limbs include lipases, proteases, and collagenases

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10
Q

Treatment for Vibrio vulnificus is:

A

Doxy + ceftazidime

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11
Q

This GNB, oxidase+, beta-hemolytic pathogen is found in fresh and chlorinated water, causes gastroenteritis in children, and can cause HUS w/ kidney failure:

A

Aeromonas hydrophila

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12
Q

This pathogen produces violet colored colonies on SBAP, starts as cellulitis, and can lead to systemic infection with visceral abscess:

A

Chromobacterium violaceum

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13
Q

Brucella requires this special media for growth:

A

Brucella agar (adds heated horse serum)

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14
Q

Helicobacter requires these special medias:

A

CAP

Brucella

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15
Q

This organism causes Tularemia and is recovered on media with cysteine such as buffered charcoal yeast extract:

A

Francisella tularensis

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16
Q

This organism requires CAMPY-BAP:

A

Campylobactr

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17
Q

Bartonella requires this special media:

A

fresh chocolate media

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18
Q

Which two species are listed under Bartonella spp>:

A

B. quintana (Trench fever)

B. henselae (cat scratch fever)

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19
Q

Causes Trench fever:

A

Bartonella quintana

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20
Q

Causes Cat scratch fever:

A

Bartonella henselae

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21
Q

How is Trench fever transmitted and what bacterium is responsible:

A

Transmitted via body lice

Bartonella quintana

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22
Q

Will Bartonella spp. grow on SBAP or MAC?

A

No. Requires fresh chocolate agar

prolonged incubation with CO2)

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23
Q

This bacterium is found in the oropharynx of rodents and causes rat bite fever:

A

Streptobacillus moniliformis

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24
Q

How is Haverhill fever transmitted, and which bacteria is responsible:

A

Ingesting contaminated milk

Streptobacillus moniliformis

25
Q

Common cause of gastritis and peptic ulcers, id via urea breath test:

A

Helicobacter pylori

26
Q

What stain is used for gastric tissue biopsies suspected of H. pylori infection:

A

Warthin-Starry silver stain

27
Q

List 4 triggers for acute transient gastritis:

A

alcohol
smoking
NSAIDS
chemo

28
Q

List 4 agents responsible for chronic gastritis:

A

H. pylori infection
alcohol
smoking
radiation

29
Q

List the 3 consequences of H. pylori infection:

A

chronic gastritis
peptic ulcer disease
gastric carcinoma

30
Q

By age 50, this organism is found in 50% of asymptomatic adults in the U.S, obtained via fecal-oral route:

A

H. pylori

31
Q

What does H. pylori secrete to buffer itself and allow survival in HCl in stomach:

A

urease

32
Q

H. pylori causes an ______ in gastric acid secretion, which can lead to ulcers:

A

increase

33
Q

List the 3 most common sites of peptic ulcers:

A

First portion of duodenum
Stomach
GE junction

34
Q

List some complications of Peptic ulcer disease:

A

Hemorrhage (anemia)
Scarring
Perforation
Malignant transformation

35
Q

How does H. pylori destroy the first line of defense in the stomach:

A

secretes proteases and phospholipases that break down glycoprotein-lipid complexes in mucous
(mucous is necessary to protect normal gastric epithelium from HCL)

36
Q

This is defined as chronic shallow ulcerations of the mucosa in any portion of the GI exposed to HCl:

A

peptic ulcer disease

37
Q

This is the most common bacterial etiologic agent:

A

Campylobacter

Salmonella is 2nd

38
Q

T/F

Campylobacter is typically associated with gastrointestinal outbreaks:

A

False

norovirus is

39
Q

How is CAMPY-BAP selective for Campylobacter:

A

contains blood + various antibiotics to suppress growth of normal fecal flora

40
Q

Campy-CVA contains which antibiotics, and is good for recovery of these two organisms:

A

cefoperazone, vancomycin, amphotericin B

*good for Arcobacter spp. and Campylobacter

41
Q

Which media can be used to improve the aerotolerance of Campylobacter:

A

CCDA

charcoal cefoperazone desoxycholate agar

42
Q

Describe colony morphology of Campylobacter:

A

Gray to pink to yellow-gray colonies

Mucoid appearing

43
Q

Campylobacter prefers incubation in these conditions:

A

microaerobic

44
Q

Campylobacter incubation temps vary by species.
C. jejuni/coli:
C. doylei:

A

C. jejuni/coli: 42 C

C. doylei: 37 C

45
Q

What is the drug of choice for Campylobacter:

A

Erythromycin

46
Q

List the 3 major pathologies of Campylobacter spp:

A

Febrile systemic disease
Periodontal disease
Gastroenteritis (C. jejuni/coli)

47
Q

Campylobacter is transmitted via:

A

ingesting contaminated food or water

48
Q

Chrysobacterium meningosepticum is now named:

A

Elizabethkingia meningoseptica

49
Q

This bacterium is associated with neonatal meningitis, and is able to survive in hospital environments, esp moist- also survives in chlorine:

A

Elizabethkingia meningoseptica

50
Q

Bordatella bronchiseptica is normal flora of:

A

dogs and cats (NOT humans)

51
Q

Does the AACCK group grow fast or slow?

A

Slow.

48 hours @ 35-37 C

52
Q

List the organisms in the HACEK group:

A 
Aggregatibacter aprophilus
Aggregatibacter actinomycetemcomitans
Cardiobacterium hominis
Eikenella corrodens
Kingella spp.
53
Q

T/F

All organisms in the HACEK group are normal flora of oral cavity, upper respiratory and GU tracts:

A

True

54
Q

This organism from the HACEK group is associated with bacteremia/endocarditis, especially with recent dental work:

A

Cardiobacterium hominis

is normal mouth flora

55
Q

This organism from the HACEK group is associated with human bite wounds and closed fist wounds, pits agar and has bleach odor:

A

Eikenella corrodens

56
Q

List the two species of Kingella:

A

K. dentrificans

K. kingae

57
Q

This organism from the HACEK group is normal flora of mouth, throat, GU, and has an affinity for bone and heart tissue, poor oral hygiene is a risk:

A

Kingella

58
Q

MAC - Ox variable:

A

Haemophilus

Micro 425 (12 decks)

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