Exam 2 - Gram- Coccobacilli (H, L, B) Flashcards
List the 3 Gram- coccobacilli:
Bordetella
Legionella
Haemophilus
This organism is capnophilic and requires X and V Factors for growth:
Haemophilus
Will Haemophilus grow on SBAP:
little to no growth (SBAP only contains X factor)
What is a special selective agar used to isolate Haemophilus influenzae in CF patients:
CAP with Bacitracin
prevents overgrowth of G+ and G- cocci
For hemolytic strains of Haemophilus (H. haemo/parahaemolyticus), what type of agar would be need to see:
Horse/rabbit blood agar
Satellite phenomenon allows for Haemophilus to grow on SBAP only if this strain is also there:
Staph aureus
since it lyses blood cells, releasing X factor, and it produces V factor on its own
Is H. ducreyi normal flora:
No
If you cant inoculate Haemophilus w/in 1 hour, you must use T-1 medium, why:
All of the species are prone to drying and temp sensitive
T/F H. influenze has capsule containing surface antigens that can be typed:
True (Types a-f)
*also has non-capsulated forms, they cause less severe infection
Which serotype of H. influenzae causes serious infections such as meningits, bacteremia, and epiglottitis:
Type B (90% of isolates) (vaccine available)
What used to be the most common cause of meningitis before its vaccine came about:
H. influenzae
What is the vaccine for H. influenzae:
Hib
What definitively ID’s H. influenzae:
ALA negative
This organism has a school of fish appearance on Gram stain:
Haemophilus ducreyi
This organism causes an STD similar to syphilis:
Haemophilus ducreyi
H. ducreyi required ID via:
PCR
T/F
H. ducreyi is common in the US:
False.
It is common in Africa, Asia, and Latin America
This organism uses protein rather than carbs for growth:
Legionella pneumophilia
This organism requires Buffered Charcoal Yeast Extract agar, and grows in the presence of L-cysteine:
Legionella pneumophilia
What are the two forms of Legionella pneumophilia:
- Dormant, cyst like (highly infectious)
* Intracellular form (evades defenses)
On BCYE, what will colonies of Legionella pneumophilia do:
fluoresce under UV light
What is required for definitive Dx for Legionella pneumophilia:
Serotyping
Is Legionella pneumophilia spread person to person:
No. infection occurs via inhalation of mist containing microbes.
(AC’s, humidifiers, hot tubs)
Which organism causes Pontiac Fever:
Legionella pneumophilia
List the 3 species of Bordetella:
B. pertussis
B. parapertussis
B. bronchiseptica
This organism has colonies resembling mercury droplets:
Bordetella pertussis/parapertussis
Does Bordetella pertussis spread person to person via resp droplets:
Yes
What is required for presumptive ID of Bordetella pertussis:
- Mercury droplet colonies
* Growth on Regan-Lowe media
What are the three stages of illness with Pertussis:
Catarrhal (7-10 days)
Paroxysmal (1-6+ wks)
Convalescent (7-10 days)
Pertussis is contagious from the ____ stage to the 3rd week of the ____ stage:
Catarrhal
Paroxysmal
This organism is the cause of kennel cough in dogs:
Bordetella bronchiseptica
What media is used to grow Bordetella bronchiseptica:
SBAP
MacConkey
Cap
What media is needed to grow Bordetella pertussis:
Bordet-Gengou
Modified Jones-Kendrick charcoal
Regan-Lowe
(will also grow on SBAP, variable growth on MacConkey)
