Exam 3 Flashcards
What do enzymes do?
speed up processes by lowering activation energy
How do enzymes do their job?
-positioning substrate for reaction
-stressing it out to be more reactive
-providing optimal environment for subtrate
What is competitive inhibtion?
beating the substrate to active site
What is noncompetitive inhibition?
binding somewhere other than the active site closing the active site, not allowing the substrate to bind to active site
What happens in allosteric regulation when it’s active?
they bind somewhere other than active site, keeping it on
What happens in allosteric regulation when it’s inactive?
bind somewhere other than active site keeping it off
what does positive feedback do?
speed up process
what does negative feedback do?
slow down/ turn off process (no more needed)
What is the effect of temperature and pH on enzymatic activity?
there is an optimal pH and temperature, cannot be too high or too low.
Name the principles of metabolic pathways
- Complex chemical reactions occur in a series of separate, intermidiate reactions that form a metabolic pathway
- Each pathway is catalyzed by a specific enzyme
- Most pathways are similar in all organisms
- In eukaryotes, most pathways are compartmentalized, making certain reactions occur in certain organelles
- key enzymes will either inhibit or activate which will determine the speed of the reaction
What are redox reactions?
transfer of electrons
What happens in reduction
electron is accepted/gained
what happens in oxidation?
electron is released
Which is in oxidized form:
NADH or NAD+
NAD+
Where does glycolysis take place?
cytoplasm
What are the products of glycolysis?
2 pyruvate
2ATP
2 NADH
Why is the process of glycolysis so long?
To not create too much energy
Why is energy released in glycolysis?
to restart the process
Where does pyruvate oxidation take place?
Mitochondrial matrix
What catalyzes pyruvate oxidation?
enzyme called pyruvate dehydrogenase
What are the products of pyruvate oxidation?
Co2
Acetyl CoA
NADH
What is Acetyl CoA used for?
Starting the citric acid cycle
Where does the citric acid cycle take place?
mitochondrial matrix
What is used to start the citric acid cycle?
Acetyl CoA
Oxaloacetate
How many times does the citric acid cycle occur?
2
What are the products of the citric acid cycle?
6 NADH
CO2
2 FADH2
Oxaloacetate