Exam 3 Flashcards
Energy
ability to do work
potential energy
associeate with position and/or configuration
what are two examples of potential energy?
chemical potential energy: stored in the bonds between atoms in a molecule
electrochemical gradient:” ions or charged molecules
kinetic energy
associated with movement
first law of thermodynamics
energy cannot be created or destroyed, energy can only be transformed or transferred ( lose and gain energy)
second law of thermodynamics
the amount of entropy in a system always increases
entropy
the amount of disorder in a system
exothermic chemical reaction: high entropy
endothermic chemical reaction: low entropy
exergonic reaction
reactants have higher potential energy than products
- energy is released = spontaneous
- hydrolysis r(x) break things apart
endergonic reaction
reactants have lower potential energy than products
- energy is absorbed = non-spontaneous
- dehydrations r(x) bring things together
ATP (adenosine triphosphate)
energy currency of the cell that fuels most endergonic reactions, energy requirments
ATP structure
- 3 negative charged phosphate groups = repel
- atp is high in potential energy (chemical potential energy)
- unstable because of the 3 PO4^-3 = ready to release E
ATP hydrolysis r(x)
ATP + H2O —(hydrolysis)–> ADP + Pi + E
Pi = inorganic phosphate
ADP = adenosine diphosphate
describe the process of energetic coupling
- start with an endergonic reactions
- add the hydrolysis of ATP (source of E)
A + B –> C
+ ATP + H20 –> ADP + Pi + E
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A+BPi (receives E)+ADP -> C+ADP+Pi = A + BPi -> C + Pi
Enzymes
proteins that catalyze chemical reactions
catalyst
increase the chemical r(x) they catalyze
- specific to the chemical r(x) they catalyze
- are not consumed in the chemical r(x) = re-used
- help reactions find each other at the right time and same place
- have an active site that is specidic to the subtrate of the r(x)
substrate
reactant in the presence of an enzyme
how do enzymes work?
enzymes lower the activation energy (Ea) of the reactions
activation energy (Ea)
energy required by EVERY reaction to overcome the transition state
transition state
when bonds are breaking in the substrates/reactants and form in the products
steps of induced fit
- active site attracts the substrate
- the enzyme undergoes a confromational change (when substrate occupy the active site)
- reaction happens = products are made
- products are released –> enzyme goes back to its original shape
what are enzymes optimal conditions and what do they depend on?
optimal conditions: temperature and pH
depends on organisms, organelle, and organ
metabolic pathways
series of steps (chemical rx) with each reaction catalyzed by a different enzyme
what happens when one of the enzymes is inactivated in a metabolic pathway?
- everything after the inactivated enzyme the concentration goes down
- the initial concentration goes up
regulation
cell shave the ability to increase/decrease the rate of a metabolic pathway