EXAM 2 - Session 16: Cellular Models for Drug Discovery

Question 1

Describe what information can be obtained by Neutral Red (NR) Assay.

Answer 1

NR weak cationic dye penetrates cell membrane and binds with anionic proteins in lysosome.
* Amount of dye is related to cell number

Question 2

What can be inferred if there is a decrease in intracellular/extractable dye?

Answer 2

Decrease in dye –> cell damage and/or dying cells
* Dye leaks from stressed/damaged lysosomes and less is detectable

Question 3

List the steps of the cell cycle.

Answer 3

M - Mitosis
Interphase: G1 to G2
* G1 - Gap 1
* (G0)
* S - synthesis of DNA
* G2 - Gap 2