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Microbiology > Exam 2 Review Questions > Flashcards

Exam 2 Review Questions Flashcards

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Biology 101 flashcards
1
Q

During the fueling reactions of metabolism, how
many precursor metabolites are produced that
are required to make macromolecules.
A.1
B.7
C.13
D.17
E.500

A

C.13

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2
Q

Which of the following is NOT a central pathway
used in metabolism of prokaryotes?
A.TCA cycle
B.Pentose Phosphate pathway
C.Calvin cycle
D.Glycolysis

A

C.Calvin cycle

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3
Q

Which of the following is a type of transport across
the cell membrane that modifies the molecule
transported in, making it unable to easily leave the
cell?
A.Group translocation
B.ABC transporters
C.Simple diffusion
D.Facilitated diffusion

A

A.Group translocation

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4
Q

Cyanobacteria gain energy from light and fix CO2,
which of the following best describes their nutritional
pattern?
A.Chemoautotroph
B.Chemoheterotroph
C.Photoautotroph
D.Photoheterotroph

A

C.Photoautotroph

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5
Q

Nitrogen assimilation is necessary for building
macromolecules, what building block does most
nitrogen flow through to be incorporated into other
building blocks?
A. ATP
B. Glutamate
C. Methionine
D. Glutamine
E. Cysteine

A

B. Glutamate

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6
Q

Which of the following is true regarding
prokaryotic DNA replication but NOT
eukaryotic DNA replication?
A. Bidirectional synthesis
B. Primers are required to begin replication
C. Replication is semiconservative
D. DNA gyrase separates concatenate
structures

A

D. DNA gyrase separates concatenate
structures

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7
Q

Many microbial cells mark their DNA to identify it
as their own. One reason for this is…
A. Protection from restriction endonucleases
B. Mark the beginning and end of operons
C. Protect the cell from helicase
D. To prevent negative supercoiling

A

A. Protection from restriction endonucleases

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8
Q

Which of the following uses a protein called
flippase?
A.Lipid translocation in the cell membrane
B.Termination of transcription
C.Transport of NAG and NAM across the cell
membrane
D.Inserting LPS in the outer membrane of gram
negative cells

A

A.Lipid translocation in the cell membrane

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9
Q

Which protein complex is responsible for
incorporating proteins specifically into the
outer membrane of gram negative
bacteria?
A. Sec proteins
B. HsiB/HsiC
C. Bam complex
D. ABC transporter
E. ClpV complex

A

C. Bam complex

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10
Q

Which secretory system forms a phage-related
contractile structure for the injection of
effector proteins into target cell?
A. Type I
B. Type II
C. Type III
D. Type IV
E. Type V
F. Type VI

A

F. Type VI

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11
Q

A _______mutation occurs when a mistake is
used made during DNA replication.
A.Induced
B.Mutagen
C.Transposable element
D.Spontaneous
E.HGT

A

D.Spontaneous

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12
Q

Which of the following is due to errors in excision
of a temperate phage from the host genome?
A.conjugation.
B.generalized transduction.
C.specialized transduction.
D.natural transformation.
E.All of the above.
BACK TO GAME

A

C.specialized transduction.

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13
Q

Conjugation between Gram (-) cells requires
which of the following?
A. lysogenic phage
B. pilus
C. capsule
D. aggregation substance

A

B. pilus

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14
Q

In the Griffith’s experiment, showing
transformation between S. pneumoniae
strains, inoculation into the mouse of which
of the following showed that it wasn’t the
capsule alone that caused disease?
a. Live R cells
b. Live S cells
c. Dead R cells
d. Dead S cells

A

d. Dead S cells

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15
Q

In the Figure, which colonies are streptomycin-
resistant and valine-requiring based on the replica
plating experiment?
a. 1, 2, 3, and 9
b. 2 and 9
c. 5 only
d. 2, 5, and 9
e. 4 only
f. None of these
answers are correct

A

c. 5 only

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16
Q

Which of the following types of regulation
would save the cell the most energy?
A. DNA topology modification
B. Attenuation
C. Proteolysis
D. RNA stability

A

A. DNA topology modification

17
Q

The catabolite repression system and the stringent
response system are examples of
__________________ that are responsible for
controlling ¾ of the protein synthesizing capacity of
E. coli cells.
A. Operons
B. Modulons
C. Regulons
D. Feederpathways
E. Electron transport chains

A

B. Modulons

18
Q

What fitness advantage do bacteria gain by
controlling enzyme amount instead of enzyme
activity?
A. All enzymes must be available all times for
viability
B. Cells do not invest energy-synthesizing proteins
that are not needed
C. The enzyme is available for immediate use
D. Less energy is required to synthesize protein
and store it than to modify activity

A

B. Cells do not invest energy-synthesizing proteins
that are not needed

19
Q

When lactose is present in an Escherichia coli
cell, which of the following is true of the lac
operon?
A. If glucose is present, the operon genes will
be expressed at a high level
B. If glucose is not present, the operon will be
expressed at a high level
C. If glucose is present, the CAP protein will
bind to the CAP binding site
D. If glucose is not present, the repressor
protein will bind to the operator

A

B. If glucose is not present, the operon will be
expressed at a high level

20
Q

Which of the following is FALSE?
A. Metabolic reactions are coordinated
B. Metabolic reactions may be controlled allosteric
interactions
C. The presence of histidine within the medium
stops synthesis of that amino acid within the
cell
D. sRNAs may bind to promoters to prevent
transcription
E. Covalent modifications always deactivate
enzymes

A

E. Covalent modifications always deactivate
enzymes

21
Q

Yeast that is used to make bread.
a. Pseudomonas aeruginosa
b. Saccharomyces cerevisiae
c. Neisseria gonorrheae
d. Borrelia burgdorferi
e. Candida albicans

A

b. Saccharomyces cerevisiae

22
Q

What system transports the murein precursors
outside of the cell membrane?
A.Transglycosylation system
B.Transpeptidation system
C.Penicillin binding proteins
D.Undecaprenylphosphate cycle

A

D.Undecaprenylphosphate cycle

23
Q

Which method of generating ATP involves the
direct transfer of a high energy PO4– to ADP
from a phosphorylated compound generates
ATP
A. Photophosphorylation
B. Substrate level phosphorylation
C. Oxidative phosphorylation
D. Product level phosphorylation

A

B. Substrate level phosphorylation

24
Q

Gram-positive bacterium
A. Pseudomonas aeruginosa
B. Chlamydia trachomatis
C. Borrelia burgdorferi
D. Plasmodium spp.
E. Rabies
F. None of the above are Gram-positive bacteria

A

F. None of the above are Gram-positive bacteria

25
Q

Which of the following bacteria are spirochetes?
A. Treponema pallidum & Borrelia burgdorferi
B. Neisseria gonorrheae & Chlamydia trachomatis
C. Candida albicans & Saccharomyces cerevisiae
D. Rabies and HSV
E. Plasmodium spp. & Pseudomonas aeruginosa

A

A. Treponema pallidum & Borrelia burgdorferi

26
Q

What are bacterial cells that are able to undergo transformation called?

A

Competent.

Microbiology (22 decks)

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