Exam 2: Calicivirus

Question 1

What are the structural components of the virus

Answer 1

Non-enveloped
Positive strand, ss RNA genome

Question 2

What is the most important gene

Answer 2

Capsid protein gene, which has hypervariable regions (C, E) which neutralize antibody epitopes and strain variation

Question 3

What is the role of Feline Calicivirus in Feline Infectious Respiratory Disease

Answer 3

40%

Question 4

What is the pathogenesis of Calicivirus

Answer 4

Exposure: Direct cat-to-cat contact and fomites
Primary virus replication: Oral, nasal, conjunctival epithelium
Viremia
Secondary virus replication

Question 5

What is the clinical presentation of FCV

Answer 5

Acute and oral respiratory disease
Nasal and ocular discharge
Conjunctivitis
Chronic stomatitis
Limping syndrome
Virulent systemic form
Feline urinary syndrome?
Subclinical

Question 6

What is the typical combination of FCV

Answer 6

Acute respiratory disease, temporarily associated with lingual ulceration

Question 7

What is virulent systemic FCV characterized by

Answer 7

Systemic inflammatory response syndrome
Disseminated intravascular coagulation (DIC)
Multi-organ failure
Mortality rates up to 67%

Question 8

What is the typical duration of clinical respiratory disease signs

Answer 8

Shedding FCV at 30 days post-infection and 50% of the cats were shedding FCV 75 days post-infection (goes beyond 2 months)

Question 9

What samples are needed for FCV

Answer 9

Oral swabs, nasal swabs, tissues
Virus isolation
DIrect FA on frozen tissue sections
RT-PCR on fresh or formalin fixed tissues
VN testing on paired sera