Exam 1 Flashcards
During the golden age of microbiology, what where scientists looking for
What causes Fermentation
what causes disease
How can we prevent disease and infection
Who was responsible for finding out that we should wash our hands
Ignaz Semmelweis
Who was responsible for creating antiseptic techniques
Joseph Lister
Who was the first to show infection control/ediology
John snow
Who was the one that created a smallpox vaccination and created the feild of immunology
Edward Jenner
What are the processes of life
Growth
Reproduction
Responsiveness
Metabolism
What are the features of prokaryotes
No nucleus
No membrane bound organelles
Circular DNA
What are the features of Eukaryotes
Have nucleus
Membrane bound organelles
Linear DNA
What are Glycocalyces
Gelatinouns sticky substance that surrounds cells
What are the two types of Glycocalyces
Capsule
Slime layer
What does the capsule glycocalyx allow bacteria to do
Evade host immune system
What does a slime layer glycocalyx allow bacteria to do
Attatch to surfaces
What do bacterial cell wall allow them to do
Withstand osmotic forces
What are bacterial cell walls composed of
Peptidoglycan
What type of bacterial cell wall has a thick layer of peptidoglycan
Gram +
What type of bacterial cell wall has a thin layer of peptidoglycan
Gram -
What colours do gram + and gram - bacteria turn in a gram stain
Gram + Purple
Gram - pink/red/magenta
How can m/c pass through a bacterial membrane passivly
Diffusion
Facilitated diffusion
Osmosis
How can m/c pass through a bacterial membrane activly
Active transport
Group translocation
What happens when a cell is placed into a hypertonic solution
The cell loses water and shinks
What happens when a cell without a cell wall is placed into a hypotonic solution
The cell takes on water and can burst
What happens when a cell with a cell wall is placed in a hypotonic solution
Water moves into the cell, but it wont burst
What are inclusion
Reserve deposits of chemicals in the cytoplasm of bacteria
What are endospores
Defensive capsule bacteria can make, so that they “hibernate” until the conditions are favorable again
How big a prokaryote ribosomes
70s
How big are eukaryote ribosomes
They have a 60s subunit and a 40s subunit
What is resolution when talking about microspoy
shortest distance between two specime that can still be distinguished by an observer
What is the point of staining for microsopy
To increase the contrast
What are the two stains used in a Gram stain
Crystal Violet (purple) Safarnin (pink)
What are the two stains used in Acid-Fast stain
Methylene blue (blue) Carbol fuchsin (red)
What are the two stains used in an endospore stain
Malachite green (green) Safranin (pink)
What is the purpose of an Acid-fast stain
to look for mycobacteria
Why do we need to use acid to stain mycobacteria
Because they have a waxy mycolic cell wall that need acid to stain
What is the colour of a positive acid-fast stain
Red = mycobacteria
What is the colour of a positive Endospore stain
Green = endospore
What do most gram + bacteria names end in
Us
ium
What are the special Gram + bacteria that dont end in Us/ium
Listeria
Nocardia
Actinomyces
Streptomyces
What do most gram - bacteria names end in
a
er
What are the special gram - bacteria that dont end in a/er
Pseudomonas Proteus Vibrio Haemophilus Bacterioides
What do the names of acid-fast bacteria have in common
Mycobacter
What bacteria gives a fried egg look
Mycoplasma
who created binomial nomenclature (genus species)
Linnaeus
Who proposed that there are 3 domains (bacteria, archaea, eukarya)
Carl Woese
What is a series of paired statments where only one of two choices applies to any particular organism, Used in determination of species
Dichotomous key
What is an aggregation of cells arising from a single parent cell
Colony
What is a collection of microbes in a complex community
Biofilm
What type of bacteria like light for energy and CO2 for carbon
Photoautotrophs
What type of bacteria like light for energy and organic compounds for carbon
Photoheterotrophs
What type of bacteria like chemical compounds for energy and CO2 for carbon
Chemoautotrophs
What type of bacteria like CHemical compounds for energy and organic compounds for carbon
Chemohetrerotrophs
What type of bacteria like cold temperatures
Psychrophilic bacteria
What type of bacteria like moderate temperatures
Mesophilic
What type of bacteria like high temperature
Thermophilic
What type of bacteria like extreme high temp
Hyperthermophilic
What type of bacteria like high (basic) pH
Basophilies
What type of bacteria like Low (acidic) pH
Acidophilies
What are the major growth requirements for microbes
Oxygen Temp pH moisture Osmotic pressure Light Food
What is a defined media
A media where all the components are known
What is a complex media
A media where the components are unknown
What is a selective media
Contain substances that favour or inhibit growth of particular organisms
What is a differential media
Visible changes in medium or differences in the appearance of colonies
What is an example of a selective media
Sabouraud dextrose arar
What is an example of differential media
Blood agar
What is an example of a Selective and differential media
MacConkey agar
What are plasmids
Extra chromosomal DNA that is found in microbes
What is the name of a fertility plasmid
F plamids
What is the name of a resistance plasmid
R plasmid
What is the name of a plasmid that allows for a bacteria to kill other bacteria
Bacteriocin plasmid
What is the name of a plasmid that allows for a bacteria to become “bad” in the human body
Virulence plasmid
What is vertical gene transfer
Organism replicates their DNA and passes it on to offspring
What is horizontal gene transfer
Transfer of DNA to another organism, or acquirement or DNA from environment
What are the 3 types of horizontal gene transfer
Transformation
Transduction
Conjugation
What type of horizontal gene transfer has the microbe take up DNA from the environemnt
Transformation
What type of gene transfer requires the cells to be considered Competent
Transformation
What is called when i virus transfers DNA from one microbe to another
Transduction
What type of DNA transfer requires a bacteria to have an F plasmid (F+) and the other to not have an F plasmid (F-)
Conjugation
What is the shape of a coccus
Sphere
What is the shape of a Bacilus
Rod
What is the shape of a Vibrio
Bat
What is the shape of a Spirochete
Spiral
What is the shape of a pleomorphic
Random
What are the three methods of Asexual reproduction
Binary fission
Snapping division
Budding
What is the result of binary fission
1 mother = 2 daughters
What is the result of snapping division
1 mother attached to a daughter (forms a hinge)
What is the result of budding
1 mother + 1 daughter
What are the different arragments of Cocci
Diplococci Streptococci Tetrads sarcinae Staphylococci