EX. Biochemical Test Results Flashcards
What are the Extracellular Enzymes?
Starch
Lipid
Casein
Gelatin hydrolysis
What is the Result for starch hydrolysis?
Clear zone (+)
Bacillus cereus
Blue coloration (-)
What is the result for Lipid hydrolysis
Clear zone (+) S. aureus
No clear zone (-)
What is the result for Casein hydrolysis?
Clear zone (+) Bacillus cereus
No clear zone (-)
What is the result for Gelatin hydrolysis?
Liquifies (>25 C, +), Proteus vulgaris
Solidifies (<25 C, -), Pseudomonas aeruginosa
What are the extracellular enzymes for Enterobacteriaceae?
Oxidase Test
Nitrate Reduction
Nitrite Reducatio test
Ortho-nitrophenyl-Beta-D-Galatopyranoside
Amino Acid Utilization
- Lysine > Cadevarine (Decarboxylase)
- Ornithine > Putresine (Decarboxylase)
- Arginine > Citrulline (Dehydrolase)
+ Moeller Decarboxylase medium
Sulfide Indole Motility
Carbohydrate fermentation
What is the result for Oxidase test?
Tetramethyl-P-phenylene diamine - dihydrochloride
Purple (+) PPAM ViC HeN
Colorless (-) Enterobacteriacheae
What is the Result for Nitrate Reduction test?
Alpha-Naphthylamine & sulfanilic acid
Red (+) Enterobacteriaceae
NO3 > NO2
What is the Result for Nitrite Reduction tst?
Colorless (+) E. faecalis
Negative (-) Red
Alpha-Naphthylamine + Sulfanilic Acid + Zinc dust
What is the Result for Ortho-Nitrophenyl-Beta-D-Galactopyranoside?
Yellow (+) for lactose fermenter (Usually detects early Lactose fermenter)
What is the Final Product of Lysine?
Cadaverine (Decarboxylase)
What is the Final Product of Ornithine?
Putrescine (Decarboxylase)
What is the Final product of Arginine
Citrulline (Dihydrolase)
What is the result for Moeller Decarboxylase Base Medium?
(+) purple Klebsiella pneumoniae
What is the result for Moeller Decarboxylase Base Medium?
(+) purple Klebsiella pneumoniae
(-) Yellow Enterobacter cloacae
What is the negative control for Lysine Decarboxylase?
Enterobacter cloacae
What is being added in Fermentation for?
Mineral oil (Anaerobic) = Yellow (Acid)
Bacteria that are positive in Arginine?
E. cloacae
E. sakazakii
What are the bacteria from SPACEd that cause blackening?
Salmonella
Proteus
(Salmonella) Arizonae
Citrobacter freundii
Edwardsiella tarda
What is the result for Motile in Sulfide Indole Motility?
Bruch like appearance or Turbidity in the inoculation site
Use to Detect gas production
Durham Tube
What are the sugars in TSI and it’s percentage?
S 10%
L 10%
G 1%
What is H2S indicator for TSI?
Ferrous Sulfate and Sodium Thiosulfate
What are the CHYSSA
Citrobacter freundii
Hafnia
Yersinia enterocolitica
Shigella sonnei
Serretia mercesens
Arizonae
From SPACEd, what is the result for the bacteria with A/A+H2S?
Citrobacter freundii
Arizonae
From SPACEd, what is the result for the bacteria with K/A+H2S?
Salmonella
Proteus
Edwardsiella tarda
From SPACEd, what is the bacteria with A/A+H2S + K/A?
Citrobacter freundii
From SPACEd, what is the bacteria with A/A+H2S + K/K?
Arizonae
From SPACEd, what is the bacteria with K/A+H2S + K/A?
Salmonella
From SPACEd, what is the bacteria with K/A+H2S + K/K?
Edwardsiella tarda
From SPACEd, what is the bacteria with A/A+H2S + R/A?
Proteus spp
From HYSS, What is the bacteria with K/K
Hafnia
Serretia marcescens
(End - End)
From HYSS, What is the bacteria with K/A
Yersinia Enterocolitica
Shigella Sonnei
What is being detected in Indole test?
Tryptophanase
What is the reagents?
Kovaks or Erlich (p-dimethyaminobenzaldehyde)
What is the End product of Methyl Red?
Mixed Acid (Acetic acid, Lactic acid, Formic Acid)
What is the media used in Methyl Red?
Clark and Lubs medium
What is the positive indicator for ,MR?
Red 4.4
What are the 2 methods for Vogues Proskauer?
Barrits
Coblentz method
Barritz method Reagents?
40% KOH and 5% alpha naphthol
Coblentz method reagents?
40% KOH with creatine and 5% alpha naphthol
What are the positive bacteria in VP?
KESH
Klebsiella (Except for Ozanae and rhinscleromatis)
Enterobacter
Serretia marcescens
Hania alvei
Bacteria that are positive in Simmons Citrate?
Enterobacter
Klebsiella
Citrobacter
Pseudomonas aeruginosa
What is Positive in Acetate utilization?
E coli (Blue)
Shigella flexneri (Green)
What s positive in MUG Test?
E. coli blue
Pseudomonas aeruginosa no flurescens
What bacteria are positive in Urease?
E. gergoviae
K. pneumoniae
K. oxytoca
Citrobacter spp
Serretia marcescens
Yersinia pseudotuberculosis
Yersinia enterocolitica
PPM
In urease, what species of Providencia is Negative?
Providencia alcalifasiens
In PPM, who is positive in Ornithine?
Morganella morgonii
What is the presumptive identification of PPM?
Pheylalanine Deaminase
Positive for Phenylalanine Deaminase?
Green
What is the nonoxidizer or non utilizer in O/F?
Alcaligenes faecalis
