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Bacte Lab (Midterm) > EX. Biochemical Test Results > Flashcards

EX. Biochemical Test Results Flashcards

1
Q

What are the Extracellular Enzymes?

A

Starch
Lipid
Casein
Gelatin hydrolysis

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2
Q

What is the Result for starch hydrolysis?

A

Clear zone (+)
Bacillus cereus

Blue coloration (-)

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3
Q

What is the result for Lipid hydrolysis

A

Clear zone (+) S. aureus

No clear zone (-)

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4
Q

What is the result for Casein hydrolysis?

A

Clear zone (+) Bacillus cereus

No clear zone (-)

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5
Q

What is the result for Gelatin hydrolysis?

A

Liquifies (>25 C, +), Proteus vulgaris

Solidifies (<25 C, -), Pseudomonas aeruginosa

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6
Q

What are the extracellular enzymes for Enterobacteriaceae?

A

Oxidase Test
Nitrate Reduction
Nitrite Reducatio test
Ortho-nitrophenyl-Beta-D-Galatopyranoside
Amino Acid Utilization
- Lysine > Cadevarine (Decarboxylase)
- Ornithine > Putresine (Decarboxylase)
- Arginine > Citrulline (Dehydrolase)
+ Moeller Decarboxylase medium
Sulfide Indole Motility
Carbohydrate fermentation

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7
Q

What is the result for Oxidase test?

A

Tetramethyl-P-phenylene diamine - dihydrochloride

Purple (+) PPAM ViC HeN
Colorless (-) Enterobacteriacheae

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8
Q

What is the Result for Nitrate Reduction test?

A

Alpha-Naphthylamine & sulfanilic acid
Red (+) Enterobacteriaceae

NO3 > NO2

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9
Q

What is the Result for Nitrite Reduction tst?

A

Colorless (+) E. faecalis

Negative (-) Red

Alpha-Naphthylamine + Sulfanilic Acid + Zinc dust

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10
Q

What is the Result for Ortho-Nitrophenyl-Beta-D-Galactopyranoside?

A

Yellow (+) for lactose fermenter (Usually detects early Lactose fermenter)

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11
Q

What is the Final Product of Lysine?

A

Cadaverine (Decarboxylase)

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12
Q

What is the Final Product of Ornithine?

A

Putrescine (Decarboxylase)

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13
Q

What is the Final product of Arginine

A

Citrulline (Dihydrolase)

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14
Q

What is the result for Moeller Decarboxylase Base Medium?

A

(+) purple Klebsiella pneumoniae

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15
Q

What is the result for Moeller Decarboxylase Base Medium?

A

(+) purple Klebsiella pneumoniae
(-) Yellow Enterobacter cloacae

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16
Q

What is the negative control for Lysine Decarboxylase?

A

Enterobacter cloacae

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17
Q

What is being added in Fermentation for?

A

Mineral oil (Anaerobic) = Yellow (Acid)

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18
Q

Bacteria that are positive in Arginine?

A

E. cloacae
E. sakazakii

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19
Q

What are the bacteria from SPACEd that cause blackening?

A

Salmonella
Proteus
(Salmonella) Arizonae
Citrobacter freundii
Edwardsiella tarda

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20
Q

What is the result for Motile in Sulfide Indole Motility?

A

Bruch like appearance or Turbidity in the inoculation site

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21
Q

Use to Detect gas production

A

Durham Tube

22
Q

What are the sugars in TSI and it’s percentage?

A

S 10%
L 10%
G 1%

23
Q

What is H2S indicator for TSI?

A

Ferrous Sulfate and Sodium Thiosulfate

24
Q

What are the CHYSSA

A

Citrobacter freundii
Hafnia
Yersinia enterocolitica
Shigella sonnei
Serretia mercesens
Arizonae

25
Q

From SPACEd, what is the result for the bacteria with A/A+H2S?

A

Citrobacter freundii
Arizonae

26
Q

From SPACEd, what is the result for the bacteria with K/A+H2S?

A

Salmonella
Proteus
Edwardsiella tarda

27
Q

From SPACEd, what is the bacteria with A/A+H2S + K/A?

A

Citrobacter freundii

28
Q

From SPACEd, what is the bacteria with A/A+H2S + K/K?

A

Arizonae

29
Q

From SPACEd, what is the bacteria with K/A+H2S + K/A?

A

Salmonella

30
Q

From SPACEd, what is the bacteria with K/A+H2S + K/K?

A

Edwardsiella tarda

31
Q

From SPACEd, what is the bacteria with A/A+H2S + R/A?

A

Proteus spp

32
Q

From HYSS, What is the bacteria with K/K

A

Hafnia
Serretia marcescens

(End - End)

33
Q

From HYSS, What is the bacteria with K/A

A

Yersinia Enterocolitica
Shigella Sonnei

34
Q

What is being detected in Indole test?

A

Tryptophanase

35
Q

What is the reagents?

A

Kovaks or Erlich (p-dimethyaminobenzaldehyde)

36
Q

What is the End product of Methyl Red?

A

Mixed Acid (Acetic acid, Lactic acid, Formic Acid)

37
Q

What is the media used in Methyl Red?

A

Clark and Lubs medium

38
Q

What is the positive indicator for ,MR?

A

Red 4.4

39
Q

What are the 2 methods for Vogues Proskauer?

A

Barrits
Coblentz method

40
Q

Barritz method Reagents?

A

40% KOH and 5% alpha naphthol

41
Q

Coblentz method reagents?

A

40% KOH with creatine and 5% alpha naphthol

42
Q

What are the positive bacteria in VP?

A

KESH

Klebsiella (Except for Ozanae and rhinscleromatis)
Enterobacter
Serretia marcescens
Hania alvei

43
Q

Bacteria that are positive in Simmons Citrate?

A

Enterobacter
Klebsiella
Citrobacter
Pseudomonas aeruginosa

44
Q

What is Positive in Acetate utilization?

A

E coli (Blue)

Shigella flexneri (Green)

45
Q

What s positive in MUG Test?

A

E. coli blue

Pseudomonas aeruginosa no flurescens

46
Q

What bacteria are positive in Urease?

A

E. gergoviae
K. pneumoniae
K. oxytoca
Citrobacter spp
Serretia marcescens
Yersinia pseudotuberculosis
Yersinia enterocolitica
PPM

47
Q

In urease, what species of Providencia is Negative?

A

Providencia alcalifasiens

48
Q

In PPM, who is positive in Ornithine?

A

Morganella morgonii

49
Q

What is the presumptive identification of PPM?

A

Pheylalanine Deaminase

50
Q

Positive for Phenylalanine Deaminase?

A

Green

51
Q

What is the nonoxidizer or non utilizer in O/F?

A

Alcaligenes faecalis

Bacte Lab (Midterm) (9 decks)

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