EX. Biochemical test for Haemophilus

Question 1

What test for Haemophilus?

Answer 1

X and V test

Question 2

What is the purpose of X and V test?

Answer 2

Differentiate Haemophilus species based on utilization of X and V growth factors

Question 3

X factor contains?

Answer 3

HEMIN or protoporphyrin IX

Question 4

V factor contains?

Answer 4

Nicotinamide Adenine dinucleotide

Question 5

Hemin is heat stable or labile

Answer 5

Stable

Question 6

Reason why Hemin is heat stable?

Answer 6

Remains in the RBC regardless if the RBC is hemolyzed or not (Which is the reason why X factor is present in CAP)

Question 7

Reason why Y factor is Heat labile?

Answer 7

Released to the RBC

Question 8

What factor is required for the growth of Haemophilues?

Answer 8

X and factor (Some only requires X)

Question 9

What media contains both X and V factors?

Answer 9

CAP

Question 10

Positive indicator for X and V test?

Answer 10

Colony surrounding on the strips of X or V or Inbetween

Question 11

What Haemophilus spp is positive on both X and V factors?

Answer 11

influenzae
aegypticus
haemolyticus

Question 12

What Haemophilus spp is positive on V factor?

Answer 12

parainfluenzae
parahaemolyticus
paraphrophilus

Question 13

What Haemophilus spp is positive on X factor?

Answer 13

ducreyi

Question 14

What Haemophilus spp is negative on both X and V factors?

Answer 14

aphrophilus