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Bacte Lab (Midterm) > 1.2.3 Autoclave Validation > Flashcards

1.2.3 Autoclave Validation Flashcards

1
Q

What are the autoclave Validation

A

Tape indicators

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2
Q

Adhesive-backed paper tape with heat sensitive, chemical indicator markings.

A

Tape indicators

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3
Q

What is the indicator for Tape indicators when they autoclaved

A

Display diagonal stripes / change colors

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4
Q

Does tape indicators indicates death of bacteria?

A

False, it only indicates 121 C

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5
Q

A bacteria that is inactivated when exposed to 121 C saturated steam for a minimum of 15 mins.

A

Geobacillus stearothermophilus

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6
Q

Interpretation of Geobacillus stearothermophilus?

A

Positive - Change color from purple to yellow
Negative - No color change

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7
Q

After sterilization process, what is the next step?

A

Break the ampule and incubate in 56 C +- 2 (24 hrs)

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8
Q

What is the interpretation of positive in Geobacillus stearothermophilus?

A

Not properly sterilize

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9
Q

What is the interpretation of Negative in Geobacillus stearothermophilus?

A

Proper sterilization (Can decontam or use the media)

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Bacte Lab (Midterm) (9 decks)

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