Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacte Lab (Midterm) > EX. Biochemical test for Non fermentative > Flashcards

EX. Biochemical test for Non fermentative Flashcards

1
Q

What are the test for NOn fermentative?

A

Acetamide utilization
Oxidation and Fermentation test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Process of utilizing acetamide?

A

Deaminase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Deaminase process occurs in the presence of what enzyme?

A

Acylamidase enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Final product of acetamide in the process of deaminase??

A

Ammonia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What media is use for acetamide utilization

A

Acetamide agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Test use to differentiate the Non fermentative organisms from Gram neg bacilli

A

Acetate utilization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the pH indicator for Acetamide utilization?

A

Bromothymol blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Positive in Acetamide utilization?

A

Pseudomonas aeruginosa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Neg in Acetamide utilization?

A

Gram neg bacilli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Oxidation requires what tube?

A

Open tube (Oxygen to enter)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Fermentation requires what tube?

A

Closed tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What type of media/agar is required for O/F test?

A

Agar deep tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How many tubes in O/F test per microorganisms?

A

2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the media required for fermentation?

A

Hugh and Leifson medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the component for Hugh and Leifson media?

A

1% CHO, 1% Agar, and peptone

Mineral oil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the pH indicator for Hugh and Leifson media?

A

Bromothymol blue

17
Q

What is the positive indicator for Fermentative organisms

A

Both tubes are yellow

18
Q

What is the Organism that has fermentation and Oxidation?

A

Enterobacteriaceae

19
Q

What is the positive indicator for Oxidizer?

A

Change color only on oxidizer tube (NOt the tube with mineral oil)

20
Q

What is the Organism that are oxidizer

A

Non fermentative organisms (Pseudomonas, Stenotrophomonas)

21
Q

What is the positive indicator for Nonoxidizer or NOnutilizer?

A

NEgative on both open and close tube

22
Q

What organisms is Nonoxidizer?

A

Alcaligenes faecalis

Bacte Lab (Midterm) (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions