EX. Biochemical test for Non fermentative Flashcards
What are the test for NOn fermentative?
Acetamide utilization
Oxidation and Fermentation test
Process of utilizing acetamide?
Deaminase
Deaminase process occurs in the presence of what enzyme?
Acylamidase enzyme
Final product of acetamide in the process of deaminase??
Ammonia
What media is use for acetamide utilization
Acetamide agar
Test use to differentiate the Non fermentative organisms from Gram neg bacilli
Acetate utilization
What is the pH indicator for Acetamide utilization?
Bromothymol blue
Positive in Acetamide utilization?
Pseudomonas aeruginosa
Neg in Acetamide utilization?
Gram neg bacilli
Oxidation requires what tube?
Open tube (Oxygen to enter)
Fermentation requires what tube?
Closed tube
What type of media/agar is required for O/F test?
Agar deep tube
How many tubes in O/F test per microorganisms?
2
What is the media required for fermentation?
Hugh and Leifson medium
What is the component for Hugh and Leifson media?
1% CHO, 1% Agar, and peptone
Mineral oil
What is the pH indicator for Hugh and Leifson media?
Bromothymol blue
What is the positive indicator for Fermentative organisms
Both tubes are yellow
What is the Organism that has fermentation and Oxidation?
Enterobacteriaceae
What is the positive indicator for Oxidizer?
Change color only on oxidizer tube (NOt the tube with mineral oil)
What is the Organism that are oxidizer
Non fermentative organisms (Pseudomonas, Stenotrophomonas)
What is the positive indicator for Nonoxidizer or NOnutilizer?
NEgative on both open and close tube
What organisms is Nonoxidizer?
Alcaligenes faecalis
