ESR and Reticulocyte count

Question 1

for assessment of rbc and erythropoietic activity

Answer 1

reticulocyte count

Question 2

supravital stains

Answer 2

new methylene blue and brilliant cresyl blue

Question 3

5th stage in the developmental series of erythrocytic cell line

Answer 3

reticulocyte

Question 4

young, premature, non nucleated rbc contain reticular materials (_____) that stain gray blue

Answer 4

RNA

Question 5

specimen requirement for reticulocyte count

Answer 5

whole blood anticoagulated with edta

Question 6

designed to reduce this labor-intesive process due to large numbers of rbc

Answer 6

miller disk

Question 7

refererence range for adults in miller disk

Answer 7

0.5-1.5

Question 8

refererence range for newborn in miller disk

Answer 8

4.0-6.00

Question 9

sources of error in miller disk

Answer 9

anemia or polycythemia

Question 10

Important reasons for DISCREPANCIES in reticulocyte counting

Answer 10

variation in the definition of reticulocytes, size of sample evaluated, type of film examined and lack of stardardized area if a calibrated is not used

Question 11

normal value of absolute reticulocyte count

Answer 11

20-115 x 10 raise to 9/L

Question 12

The premature reticulocytes are called

Answer 12

stress reticulocytes

Question 13

correction for both the RBC count and the presence of stress reticulocytes value obtained is called the

Answer 13

reticulocyte production index

Question 14

An adequate bone marrow response usually is indicated by an RPI that is greater than 3. An
inadequate erythropoietic response is seen when the RPI is less than 2.

Answer 14

reference interval

Question 15

this test is reported in mm/hr

Answer 15

esr test

Question 16

This test determines the rate at which erythrocytes fall to
the bottom of a vertical tube of anticoagulated blood
within a specific period.

Answer 16

erytrocyte sedimentation rate

Question 17

Measure of magnitude of electrostatic charge or repulsion/attraction between particles

Answer 17

zeta potential

Question 18

Zeta potential results from negatively charged _____________________

Answer 18

sialic acid groups on rbc’s membrane

Question 19

three stages of esr

Answer 19

rouleaux formation, sedimentation , packing

Question 20

first 10 mins, piling up of RBC’s take place

Answer 20

rouleaux formation

Question 21

40 minutes, Settling of RBC’s occurs at constant rate

Answer 21

sedimentation

Question 22

10 minutes, sedimentation slows and cells start to pack at the bottom of the tube

Answer 22

packing

Question 23

factors influencing esr

Answer 23

plasma, red cell, mechinal and technical factor

Question 24

in mechanical factor, a tilf of ____: cause error up to ____ %

Answer 24

3 degrees , 30

Question 25

This test measures the distance that RBCs will fall in a vertical tube over a given time period.

Answer 25

westergren method

Question 26

this is the international reference method

Answer 26

westergren method

Question 27

It can be used for both ESR and hematocrit determination

Answer 27

wintrobe and landsberg method

Question 28

normal value of wintrobe and landsberg method in women

Answer 28

0-20 mm/hr

Question 29

normal value of wintrobe and landsberg method in men

Answer 29

0-9 mm/hr

Question 30

normal value of wintrobe and landsberg method in children

Answer 30

0-13 mm/hr

Question 31

Used in case of infants and if the blood is inadequate

Answer 31

micro-esr method

Question 32

0.2 mL blood to fill a plastic disposable tube _____________________

Answer 32

230 mm long with a 1 -mm internal bore

Question 33

micro-esr method utilizes whole blood to completely fill _____________

Answer 33

75-mm heparanized microhematocrit capillary tube