erasmus test

Question 1

FISH (exact process)

Answer 1

a semen staining method that colours the nucleus of the sperm without killing it
used for sex separating the semen for insemination
uses a DNA probe and target sequence
fluoresence in situ hybridization
reliable, accurate, expensive

Question 2

Nucleus transfer - why not identical clones

Answer 2

foreign nucleus into enucleated oocyte + fusion + activation

cytoplasmic (mithochondrial) DNA 5-10% difference

Question 3

The only successful process of sexifying sperm

Answer 3

FISH - accurate, reliable, expensive
flow cytometry - reliable but slow and expensive
unreliable:(centrifugation, sedimentation, ELFO, timed AI)

Question 4

Where in cow embryo transfer

Answer 4

uterus

Question 5

Which AI method is not used in ewes

Answer 5

rectovaginal

Question 6

Consequences of spaying in diestrus

pyometra
nothing
obesity
pregnancy
pseudopregnancy

Answer 6

pseudopregnancy

Question 7

Which is incorrect about mammary tumors ->

(dont know the whole q or answers, this is a similar pq:)

Which statement is FALSE concerning the inflammatory mammary carcinoma?

Answer 7

Most often anaplastic carcinoma
Is an immediate surgical indication
Often develops in the luteal phase
If it develops, we see symptoms typical of severe mastitis and dermatitis

Question 8

Which is true about obesity after spaying ->

Answer 8

metabolic rate slows down

Question 9

Number of fractions in dog

Answer 9

3

Question 10

Volume of 2nd fraction in dog

Answer 10

5-6

Question 11

eosin staining

Answer 11

stain dead sperm cells?

Question 12

What if sperm is less opaque ->

Answer 12

less spermatozoa than there should be

Question 13

Which accessory glands in horse ->

Answer 13

all of them (ampulla, vesicular gland, prostate, bulbourethral gland/cowper)

Question 14

where AI in cow

Answer 14

intrauterine

Question 15

First thing to do in orphan puppy

Answer 15

check the temperature

Question 16

Which is true about pyometra ->

Answer 16

something with E.coli ? aner ikke hva de vil frem til

Question 17

17. FSH targets which cells

Answer 17

sertoli

Question 18

18. Which cells produce testosterone

Answer 18

Leydig

Question 19

What is Necrozoospermia ?

Answer 19

sperm are dead in a fresh sample,

there are grades: moderate 50-80%, severe >80%

Question 20

Accuracy of assays (FISH, flow)

Answer 20

FISH - accurate, reliable, expensive
flow cytometry - reliable but slow and expensive
unreliable: (centrifugation, sedimentation, ELFO, timed AI)

Question 21

GNRH surge center in males

Answer 21

there is no surge center

Question 22

peroxidase test (the aim )

Answer 22

aim: to diferentiate the round cells in the ejaculate. neutrophil granulocyte vs spermatid

Question 23

swelling test - tail swelling - what is normal?

Answer 23

normal if 60% responds

Question 24

What happens in slow cooling of sperm?

Answer 24

its a part of the semen preparation. collect, dilute, rapid cool to 20 degree, slow cool to 0,5/min to storage temp

Question 25

26. Which accessory gland most important in dogs

Answer 25

• prostate

Question 26

27. temperature of liquid nitrogen

Answer 26

-196°C

Question 27

28. what do you do under high magnitude

Answer 27

individual movement (according to presentation)

Question 28

29. tight junction

Answer 28

blood-testis barrier

Question 29

30. what if the insemination happens too late after the heat

Answer 29

aging of the oocyte?