erasmus test Flashcards
FISH (exact process)
a semen staining method that colours the nucleus of the sperm without killing it
used for sex separating the semen for insemination
uses a DNA probe and target sequence
fluoresence in situ hybridization
reliable, accurate, expensive
Nucleus transfer - why not identical clones
foreign nucleus into enucleated oocyte + fusion + activation
cytoplasmic (mithochondrial) DNA 5-10% difference
The only successful process of sexifying sperm
FISH - accurate, reliable, expensive
flow cytometry - reliable but slow and expensive
unreliable:(centrifugation, sedimentation, ELFO, timed AI)
Where in cow embryo transfer
uterus
Which AI method is not used in ewes
rectovaginal
Consequences of spaying in diestrus
pyometra nothing obesity pregnancy pseudopregnancy
pseudopregnancy
Which is incorrect about mammary tumors ->
(dont know the whole q or answers, this is a similar pq:)
Which statement is FALSE concerning the inflammatory mammary carcinoma?
Most often anaplastic carcinoma
Is an immediate surgical indication
Often develops in the luteal phase
If it develops, we see symptoms typical of severe mastitis and dermatitis
Which is true about obesity after spaying ->
metabolic rate slows down
Number of fractions in dog
3
Volume of 2nd fraction in dog
5-6
eosin staining
stain dead sperm cells?
What if sperm is less opaque ->
less spermatozoa than there should be
Which accessory glands in horse ->
all of them (ampulla, vesicular gland, prostate, bulbourethral gland/cowper)
where AI in cow
intrauterine
First thing to do in orphan puppy
check the temperature
Which is true about pyometra ->
something with E.coli ? aner ikke hva de vil frem til
- FSH targets which cells
sertoli
- Which cells produce testosterone
Leydig
What is Necrozoospermia ?
sperm are dead in a fresh sample,
there are grades: moderate 50-80%, severe >80%
Accuracy of assays (FISH, flow)
FISH - accurate, reliable, expensive
flow cytometry - reliable but slow and expensive
unreliable: (centrifugation, sedimentation, ELFO, timed AI)
GNRH surge center in males
there is no surge center
peroxidase test (the aim )
aim: to diferentiate the round cells in the ejaculate. neutrophil granulocyte vs spermatid
swelling test - tail swelling - what is normal?
normal if 60% responds
What happens in slow cooling of sperm?
its a part of the semen preparation. collect, dilute, rapid cool to 20 degree, slow cool to 0,5/min to storage temp
- Which accessory gland most important in dogs
- prostate
- temperature of liquid nitrogen
-196°C
- what do you do under high magnitude
individual movement (according to presentation)
- tight junction
blood-testis barrier
- what if the insemination happens too late after the heat
aging of the oocyte?
