Equine Flashcards
What is the birth date of all foals?
1st January
How long is gestation?
11 months
Which months should covering take place in?
Feb-June/July
What kind of oestrus do horses show?
Seasonally polyoestrus (Spring-Autumn)
How long is the oestrus cycle?
21-23 days
How long is the oestrus period?
2-4 days
How long is the dioestrus period?
15-17 days
How does melatonin affect GnRH?
Antagonises
From when do you give the mare artificial light?
15th December
How long does light take to work in the mare?
6 weeks
Do you give dopamine or dopamine antagonists to induce breeding?
Antagonists
Why do you give progesterone during transitional oestrous?
May not involve ovulation so progesterone stops cycling then taking this away causes full oestrus
What can you give to mimic dioestrus?
PGs
Why might you use intrauterine glass marbles to mimic pregnancy and stop cycling?
Because can’t give drugs to a racehorse
Which vaccine can you give to working animals to stop cycling?
Porcine GnRH
Which three things does venereal disease screening look for?
Taylorella (CEM), Pseudomonas, Klebsiella
Which transport medium do you use for a clitoral swab?
Amies charcoal
When is the only time you can get an endometrial swab?
In oestrus because cervix is open here
How can mares wink clitoris?
Because have circular and longitudinal muscle
Which orientation should the anus and vulva be?
Vertical
What happens to the anus with age?
Sinks inwards
Which seals prevent infection entry?
Vulval and vaginal
Where should the pelvis shelf be?
At the top of the vulval opening
What do endometrial epithelial cells look like?
Long and large with a wispy cytoplasm
What do vaginal/cervical cells look like?
Keratinised
What does the ovary look like on ultrasound if in season?
Orange segment and ovulation fossa with one large follicle
What three things can you check for 48 hours after mating?
Ovulation, twin ovulation, fluid in uterus
What happens if inflammatory response has not resolved after 48 hours?
Mare is “susceptible” so cover only once here
Above what age is endometriosis normal?
9
What are three changes that occur with age?
Gland “nests”, fibrosis, excessive lymphatic lacunae
For how long is stallion sperm viable?
72 hours
How often should you examine for oestrus?
24-48 hr
Why do mares need boots?
To prevent kicking
Why do mares need a rubber ring?
For stallion biting
Where does fertilisation occur?
The oviducts
How long does it take for the conceptus to enter the uterus?
4-6 days
What months have peak testosterone?
April to May
How long does spermatogenesis take?
56 days
What is one problem with testicle damage?
Causes 2 months infertility
What is the lead rope like in stallions?
Longer
Why are teaser stallions used?
To avoid actual stallion getting kicked
What is one problems with scrotal damage?
Anti-sperm antibodies
How many degrees can the testicle swing without injury?
90-180
What happens if the testicle twists too far?
Pampiniform plexus occludes
What correlates with the daily sperm output?
Size of testicle
What does daily sperm output determine?
Number of mares can breed with
What can cryptorchids produce large amounts of?
Androgens
Which breeds commonly get cryptorchid?
TB and Welsh Pony
What are the two types of cryptorchid?
Partial and complete
How can you tell if any testicular tissue has been left behind after castration?
Anti-mullerian hormone levels
Which accessory glands do horses have?
All four
How many thrusts does ejaculation take?
3-5 (up to 8)
Which places do you swab in the stallion?
Urethra, urethral fossa, penile sheath
What is a high risk for sedation?
Acepromazine
What temperature should the artificial vagina be?
44-48 degrees
Where is the gel in the semen from?
The seminal vesciles
Why must you use a bottle warmers for the sperm?
To prevent cold shock and to keep dark
Why should you wash but not disinfect the stallion before collection?
Irritates and removes natural flora
Which kind of mares can you use for live mare collection?
Oestrus, PG, IM oestradiol, ovariectomised, dummy
Which side should handlers and collectors stand?
The same side
For how many days should you collect ejaculate?
5-7 days
What can you do instead of going every 5-7 days?
Collect two samples an hour apart and use the second one
What is the second sample like compared to the first?
50% less
How should you evaluate the semen and in what order?
Volume, colour, pH, motility, conc, morphology, cytology, bacteriology
What should semen volume be?
70ml
What should semen pH be?
7.2-7.6ml
Why should you test the pH quickly?
Metabolic activity of sperm increases it
What happens to motility over time?
Decreases
Why do you add extender to the semen?
Can clump
How many semen should be moving?
75-90%
How many semen should be moving straight?
55-80%
How many sperm should still be moving after 6 hours?
10%
How many should still be moving after 24 hours in extended semen?
10%
Which stain do you use to see the live/dead ratio?
Nigrosin/eosin
What % should be live?
80%
What % should be morphologically normal?
70%
How do you work out the TNM?
total number x % morphologically normal x % progressively motile
Where do primary sperm defects occur?
Within the testes e.g. extra heads, bent
Where do secondary defects occur?
Within the epididymis and efferent tracts e.g. retained droplet
What is success rate per cycle with chilled semen?
55-70%
What is success rate per cycle with frozen semen?
35-59%
After which time limit must you remove gel and use extender?
If not using semen after 10 mins
After which time limit must you remove seminal plasma and use light and air proof container?
If storing for 2-6 hours
Why do you remove seminal plasma?
To decrease capacitation
What do you use if chilling for use in the next couple of days?
An equitainer
How quickly does an equitainer cool the semen?
0.3 degrees per minute
What temperature does an equitainer keep semen at?
4-6 degrees for 3 days
What is an example of a cryoprotectant?
Glycerol
What temperature is semen frozen at?
-196
Why must straws all freeze at the same time?
To avoid cellular damage
What is the volume of a straw?
0.5ml
What do larger straws have in the middle?
A hole
What must the motility be in frozen semen?
30%
Which mares are the hardest to breed from?
Old, competing
When should you inseminate fresh semen?
Up to 48 hours before ovulation
When should you inseminate chilled semen?
Up to 24 hours before ovulation
When should you inseminate frozen semen?
From 6 hours before to 6 hours after ovulation (usually before)
What must dummy mares have at the front?
A head
Why don’t some stallions like dummy mares?
Rubs their knees