Epigentics

Question 1

Histone acetylation

Answer 1

Removes + side chain charge on lysine –> chromatin is less tightly bound to histones and can be transcribed.

Question 2

DNA methylation

Answer 2

Typically occurs at CpG islands in promoters via DNA methyltransferases, reducing transcription by recruiting deactylases to convert from euchromatin to heterochromatin.

Question 3

DNase-seq

Answer 3

DNase digests and sequences euchromatin –> peaks and valleys on DNase seq based on number of reads within a sequence, tells you which regions are available for transcription and which are being silenced

Question 4

MethylC-seq

Answer 4

Treat DNA with bisulfite, will convert unmethylated cytosine to uracil but not methylated –> can analyze methylated areas in the sequence for expected epigenetic modifications vs. pathological ones