Enzymes and DNA manipulation Flashcards
Enzyme
a protein that speeds up chemical reaction (catalyses) via lowering the activation energy
substrate
binds to enzymes active site, and is reversible
Enzyme substrate complex
an Enzyme and substrate binded together
4 factors that effect enzymes
Temprature
Ph / acidity
concentration of enzymes
concentration of sub strates
denature
enzymes lose there shape due to bad condittions stopping it from working
inhibitors
stops enzymes from binding to substrates via blocking the active cite
competitive inhibitors
inhibitor binds to active site blocking substrate from binding, but of substrate concentration increases it will get removed
Irreversible
competitive inhibitor that not effected by substrate concentration
reversible inhibitors
Competitive inhibitors but when concentration of substrate builds up they are removed from the enzyme
non-competitive inhibitors
inhibitor binds to allosteric site, changing the shape of the active site.
allosteric site
the location on the enzyme where non-competitive inhibitors bind
Transgenic
DNA inserted from different species
Sisgenic
DNA from same species