Enzymes Flashcards
Are physically distinct versions of a given enzyme, each of which catalyzes the same reaction
Isozymes
True or false. Enzymes lower free energy of the equilibrium of the reaction
False. Enzymes lower free energy of activation not reactants, products and equilibrium of reaction
Catalyzes cleavage of C-C, C-O, C-N and other bonds by atom elimination, leaving double bonds
Lyases
Catalyze geometric or structural changes within a molecule
Isomerases
Catalyze the joining together of two molecules coupled to the hydrolysis of ATP
Ligases
Which equation uses this formula?
Michaelis Menten Equation
Describes First Order Reaction.
Point A. As substrate increases, velocity increases
Describes Zero Order Reaction
Point C. No increase in substrate can increase the rate of velocity due to saturation.
Substrate concentration at which Vmax/ 2.
Point B. Michaelis Constant (Km)
Factors that affect rate of reaction
Substrate Concentration Temperature pH
Used to calculate Km and Vmax as well as to determine the mechanism of action of enzyme inhibitors
Lineweaver Burk plot
Competitive or Noncompetitive Inhibition: Can be reversed by increasing the [S]
Competitive
Competitive or Noncompetitive Inhibition: Increased Km
Competitive
Competitive or Noncompetitive Inhibition: Lowered Vmax
Noncompetitive
Competitive or Noncompetitive Inhibition: Binds at primary site
Competitive
Competitive or Noncompetitive Inhibition: Kmax unchanged
Noncompetitive
Competitive or Noncompetitive Inhibition: Can be reversed by increasing [E]
Noncompetitive
Catalyze transfer of phosphate group from a high-energy molecule to a substrate
Kinase
Adds inorganic phosphate onto substrate without using ATP
Phosphorylase
Removes phosphate group from substrate
Phosphatase
Catalyze oxidation-reduction reactions
Dehydrogenase
Adds hydroxyl group onto substrate
Hydroxylase
Transfer CO2 groups with the help of biotin
Carboxylase
Relocates a functional group within a molecule
Mutase
